FlyBase Allele Report: Dmel\Mitf[KK113614]

General Information

Symbol

Dmel\Mitf^KK113614

Species

D. melanogaster

Name

FlyBase ID

FBal0238764

Feature type

allele

Associated gene

Dmel\Mitf

Associated Insertion(s)

Carried in Construct

P{KK113614}

Also Known As

UAS-Mitf^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK113614}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK113614

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of lysosomal storage disease

CEA

(Bouché et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain, with Scer\GAL4^elav.PU

(Bouché et al., 2016)

adult brain, with Scer\GAL4^ey-OK107

(Bouché et al., 2016)

autophagosome | adult stage, with Scer\GAL4^elav.PU

(Bouché et al., 2016)

developing neuron | ectopic | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

(Zhang et al., 2024)

developing neuron | ectopic | larval stage | somatic clone - tissue specific, with Scer\GAL4^GMR46D04, Scer\GAL4^c311

(Zhang et al., 2024)

eye disc | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

(Zhang et al., 2024)

eye disc | larval stage | somatic clone - tissue specific, with Scer\GAL4^GMR46D04, Scer\GAL4^c311

(Zhang et al., 2024)

fat body | third instar larval stage, with Scer\GAL4^Lsp2.PH

(Bouché et al., 2016)

lysosome | adult stage, with Scer\GAL4^elav.PU

(Bouché et al., 2016)

lysosome | third instar larval stage, with Scer\GAL4^Lsp2.PH

(Bouché et al., 2016)

mitochondrion | adult stage, with Scer\GAL4^elav.PU

(Bouché et al., 2016)

peripodial epithelium of eye disc | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

(Zhang et al., 2024)

peripodial epithelium of eye disc | larval stage | somatic clone - tissue specific, with Scer\GAL4^GMR46D04, Scer\GAL4^c311

(Zhang et al., 2024)

Detailed Description

Statement

Reference

Ectopic expression of Mitf^KK113614 RNAi under the control of Scer\GAL4^ey-OK107 results in a significant accumulation of polyubiquitinated protein aggregates and the expression under Scer\GAL4^elav.PU leads to significantly increased size of mitochondria in the brain of adult flies upon starvation.

The fat body of third instar larvae expressing Mitf^KK113614 RNAi under the control of Scer\GAL4[Lsp2.PH]) shows accumulation of the ref(2)P protein regardless of their fed/starved status.

Ectopic expression of Mitf^KK113614 RNAi under the control of Scer\GAL4^Lsp2.PH results in a significantly decreased number of acidic vesicles in the fat body of starved third instar larvae, while the number of lysosomes is significantly increased in fat bodies of fed but not starved larvae compared to controls. Mitf^KK113614 RNAi expression under also prevents normal accumulation of lipid droplets in fed larvae: both fed and starved larvae display the same (low) amount of lipid droplets as starved wild-type controls.

Ectopic expression of Mitf^KK113614 RNAi under the control of Scer\GAL4^elav.PU results in mild accumulation of lysosome-like structures and autophagosomes in the brains of fed adult flies compared to controls and leads to increased size of lysosome-like structures and the appearance of lamellar bodies as well as significantly decreased number of autophagosomes in the brains of starved flies compared to starved controls.

Ectopic expression of Mitf^KK113614 RNAi under the control of Scer\GAL4^Lsp2.PH results in significantly decreased level of lysosome-autophagosome fusion in starved third instar larvae compared to starved wild-type but the fusion level in fed larvae compared to fed controls is unchanged.

(Bouché et al., 2016)

Expression of Mitf^KK113614 under the control of Scer\GAL4^byn-Gal4 leads to early larval lethality.

(Zhang et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Mitf^KK113614, Scer\GAL4^Act5C.PI has abnormal neuroanatomy | larval stage | somatic clone - tissue specific phenotype, non-enhanceable by Cka[+]/Cka^s1883