FlyBase Allele Report: Dmel\ebd1[JF02926]

General Information

Symbol

Dmel\ebd1^JF02926

Species

D. melanogaster

Name

FlyBase ID

FBal0239431

Feature type

allele

Associated gene

Dmel\ebd1

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02926}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02926}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02926

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number, with Scer\GAL4^NP0001

(Tian et al., 2017)

increased occurrence of cell division, with Scer\GAL4^NP0001

(Tian et al., 2017)

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^NP0001

(Tian et al., 2017)

enteroblast | adult stage | increased number, with Scer\GAL4^NP0001

(Tian et al., 2017)

intestinal stem cell | adult stage | increased number, with Scer\GAL4^NP0001

(Tian et al., 2017)

Detailed Description

Statement

Reference

Adult flies expressing ebd1^JF02926 under the control of Scer\GAL4^{Myo31DF-NP0001} (combined with Gal80[ts] to limit the time of expression to adulthood) display increased numbers of progenitor cells and intestinal stem cells that are grouped in chains or clusters as well as their increased mitotic activity. No effect on ISC proliferation is observed when the RNAi is expressed during development.

(Tian et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP0001, ebd1^JF02926 has increased cell number phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^HMS00948

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has increased cell number phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^HMS00901

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has increased cell number phenotype, suppressible by upd3^GD6811/Scer\GAL4^NP0001

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has increased occurrence of cell division phenotype, suppressible by Scer\GAL4^NP0001/upd3^HM05061

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has increased cell number phenotype, suppressible by Scer\GAL4^NP0001/upd3^HM05061

(Tian et al., 2017)

Suppressor of

Statement

Reference

ebd1^JF02926, Scer\GAL4^NP7397 is a suppressor of increased cell number | adult stage phenotype of Apc^GD1333, Scer\GAL4^NP7397

(Tian et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^NP0001, ebd1^JF02926 has intestinal stem cell | increased number | adult stage phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^HMS00948

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has adult midgut epithelium phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^HMS00901

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has intestinal stem cell | increased number | adult stage phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^HMS00901

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has adult midgut epithelium phenotype, suppressible by upd3^GD6811/Scer\GAL4^NP0001

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has intestinal stem cell | increased number | adult stage phenotype, suppressible by upd3^GD6811/Scer\GAL4^NP0001

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has adult midgut epithelium phenotype, suppressible by Scer\GAL4^NP0001/upd3^HM05061

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has intestinal stem cell | increased number | adult stage phenotype, suppressible by Scer\GAL4^NP0001/upd3^HM05061

(Tian et al., 2017)

Scer\GAL4^NP0001, ebd1^JF02926 has adult midgut epithelium phenotype, suppressible | partially by Scer\GAL4^NP0001/upd2^HMS00948

(Tian et al., 2017)

Suppressor of

Statement

Reference

ebd1^JF02926, Scer\GAL4^NP7397 is a suppressor of adult midgut epithelium phenotype of Apc^GD1333, Scer\GAL4^NP7397

(Tian et al., 2017)

ebd1^JF02926, Scer\GAL4^NP7397 is a suppressor of intestinal stem cell | increased number | adult stage phenotype of Apc^GD1333, Scer\GAL4^NP7397

(Tian et al., 2017)

ebd1^JF02926, Scer\GAL4^NP7397 is a suppressor of enteroblast | increased number | adult stage phenotype of Apc^GD1333, Scer\GAL4^NP7397

(Tian et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of intestinal stem cells in the midgut of adult flies expressing ebd1^JF02926 under the control of Scer\GAL4^{Myo31DF-NP0001} (combined with Gal80[ts] to limit the time of expression to adulthood) can be suppressed (to a varying degree) by co-expression of any of the following: upd2^HMS00948, upd2^HMS00901, upd3^GD6811 or upd3^HM05061 (which also significantly reduces the number of mitotic cells in the adult midgut).

The increased number of progenitor cells (intestinal stem cells and enteroblasts) observed in adult flies expressing Apc^GD1333 under the control of Scer\GAL4^esg-NP7397 is fully suppressed by co-expression of ebd1^JF02926.

(Tian et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28296

y¹ v¹; P{TRiP.JF02926}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG3371^JF02926

ebd1^JF02926

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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