FlyBase Allele Report: Dmel\upd2[HMS00901]

General Information

Symbol

Dmel\upd2^HMS00901

Species

D. melanogaster

Name

FlyBase ID

FBal0257576

Feature type

allele

Associated gene

Dmel\upd2

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00901}

Also Known As

UAS-Upd2^RNAi, UAS-upd2 RNAi#2, Upd2-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00901}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00901

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intestinal cancer

modeled by Sox21a⁶

(Zhai et al., 2015)

modeled by yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased size | adult stage | somatic clone, with Scer\GAL4^R42A07.-FRT

(Harris et al., 2020)

increased cell number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

increased cell number | larval stage, with Scer\GAL4^NP1029

(Destalminil-Letourneau et al., 2021)

Phenotype Manifest In

adult anterior midgut epithelium, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Zhai et al., 2015)

embryonic/larval crystal cell | increased number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

embryonic/larval crystal cell | increased number | larval stage, with Scer\GAL4^NP1029

(Destalminil-Letourneau et al., 2021)

intestinal stem cell, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Zhai et al., 2015)

Detailed Description

Statement

Reference

Expression of upd2^HMS00901 under the control of Scer\GAL4^esg-NP5130 (limited to the adult stages using Scer\GAL80^ts.αTub84B) leads to a decrease in intestinal stem cell numbers in the anterior midgut.

(Zhai et al., 2015)

Adults expressing upd2^HMS00901 under the control of Scer\GAL4^dome-PG14 have normal triacylglycerol levels.

(Rajan and Perrimon, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^R42A07.-FRT/upd2^HMS00901 is an enhancer of visible | adult stage | somatic clone phenotype of Ecol\lexA^DBD.R85E08, Hsap\RELA^AD.Hsp70, rpr^{13xlexAop.cHa}

(Harris et al., 2020)

Scer\GAL4^R42A07.-FRT/upd2^HMS00901 is an enhancer of decreased size | adult stage | somatic clone phenotype of Ecol\lexA^DBD.R85E08, Hsap\RELA^AD.Hsp70, rpr^{13xlexAop.cHa}

(Harris et al., 2020)

Suppressor of

Statement

Reference

upd2^HMS00901, Scer\GAL4^esg.PU is a suppressor of hyperplasia | adult stage phenotype of N^DN.UAS.cUa, Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

Scer\GAL4^NP0001/upd2^HMS00901 is a suppressor | partially of increased cell number phenotype of Scer\GAL4^NP0001, ebd1^JF02926

(Tian et al., 2017)

Scer\GAL4^NP0001/upd2^HMS00901 is a suppressor | partially of neoplasia phenotype of Sox21a⁶

(Zhai et al., 2015)

upd2^HMS00901, Scer\GAL4^ptc-559.1 is a suppressor | partially of hyperplasia phenotype of N^UAS.icd, Scer\GAL4^ptc-559.1

(Djiane et al., 2013)

NOT Suppressor of

Statement

Reference

upd2^HMS00901/Scer\GAL4^esg.PU is a non-suppressor of increased cell number | adult stage phenotype of Scer\GAL4^esg.PU, botv^GD2083

(Ma et al., 2019)

upd3^HMS00646, upd2^HMS00901, Scer\GAL4^esg.PU is a non-suppressor of increased cell number | adult stage phenotype of Scer\GAL4^esg.PU, botv^GD2083

(Ma et al., 2019)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^R42A07.-FRT/upd2^HMS00901 is an enhancer of wing | somatic clone phenotype of Ecol\lexA^DBD.R85E08, Hsap\RELA^AD.Hsp70, rpr^{13xlexAop.cHa}

(Harris et al., 2020)

Suppressor of

Statement

Reference

upd2^HMS00901/Scer\GAL4^esg.PU is a suppressor of adult posterior midgut phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

Scer\GAL4^NP0001/upd2^HMS00901 is a suppressor | partially of adult midgut epithelium phenotype of Scer\GAL4^NP0001, ebd1^JF02926

(Tian et al., 2017)

Scer\GAL4^NP0001/upd2^HMS00901 is a suppressor | partially of intestinal stem cell | increased number | adult stage phenotype of Scer\GAL4^NP0001, ebd1^JF02926

(Tian et al., 2017)

Scer\GAL4^NP0001/upd2^HMS00901 is a suppressor | partially of adult anterior midgut epithelium phenotype of Sox21a⁶

(Zhai et al., 2015)

NOT Suppressor of

Statement

Reference

upd2^HMS00901/Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^esg.PU, botv^GD2083

(Ma et al., 2019)

upd3^HMS00646, upd2^HMS00901, Scer\GAL4^esg.PU is a non-suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^esg.PU, botv^GD2083

(Ma et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of intestinal stem cells in the midgut of adult flies expressing ebd1^JF02926 under the control of Scer\GAL4^{Myo31DF-NP0001} (combined with Gal80[ts] to limit the time of expression to adulthood) can be partially suppressed by co-expression of upd2^HMS00901.

(Tian et al., 2017)

Expression of upd2^HMS00901 reduces tumor formation in the anterior midgut when N^KK102890 is expressed under the control of Scer\GAL4^esg-NP5130 (limited to the adult stages using Scer\GAL80^ts.αTub84B).

(Zhai et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33949