FlyBase Allele Report: Dmel\upd2[HMS00948]

General Information

Symbol

Dmel\upd2^HMS00948

Species

D. melanogaster

Name

FlyBase ID

FBal0257618

Feature type

allele

Associated gene

Dmel\upd2

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00948}

Also Known As

UAS-upd2-RNAi, UAS-upd2 RNAi#1

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00948}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00948

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of congenital leptin deficiency

CEA

(Beshel et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intestinal cancer

modeled by yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

Phenotype Manifest In

embryonic/larval crystal cell | increased number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

Detailed Description

Statement

Reference

Expression of upd2^HMS00948 under the control of Scer\GAL4^Cg.PA results in significantly reduced weight in adult flies but it has no effect on food consumption or attraction to food cues. Scer\GAL4^elav.PU-driven expression also does not affect food attraction or intake, but the average adult body weight is slightly increased relative to controls.

(Beshel et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

upd2^HMS00948, Scer\GAL4^esg.PU is a suppressor of hyperplasia | adult stage phenotype of N^DN.UAS.cUa, Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

Scer\GAL4^NP0001/upd2^HMS00948 is a suppressor | partially of increased cell number phenotype of Scer\GAL4^NP0001, ebd1^JF02926

(Tian et al., 2017)

NOT Suppressor of

Statement

Reference

upd2^HMS00948, Scer\GAL4^ptc-559.1 is a non-suppressor of hyperplasia phenotype of N^UAS.icd, Scer\GAL4^ptc-559.1

(Djiane et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

upd2^HMS00948/Scer\GAL4^esg.PU is a suppressor of adult posterior midgut phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

Scer\GAL4^NP0001/upd2^HMS00948 is a suppressor | partially of adult midgut epithelium phenotype of Scer\GAL4^NP0001, ebd1^JF02926

(Tian et al., 2017)

Scer\GAL4^NP0001/upd2^HMS00948 is a suppressor | partially of intestinal stem cell | increased number | adult stage phenotype of Scer\GAL4^NP0001, ebd1^JF02926

(Tian et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of intestinal stem cells in the midgut of adult flies expressing ebd1^JF02926 under the control of Scer\GAL4^{Myo31DF-NP0001} (combined with Gal80[ts] to limit the time of expression to adulthood) can be partially suppressed by co-expression of upd2^HMS00948.

(Tian et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33988

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00948}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Upd2^RNAi

(Ma et al., 2019)

upd2^HMS00948

upd2^i-2

(Cao et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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