FlyBase Allele Report: Dmel\yki[RNAi.N.UAS]

General Information

Symbol

Dmel\yki^RNAi.N.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0239889

Feature type

allele

Associated gene

Dmel\yki

Associated Insertion(s)

Carried in Construct

P{UAS-yki.RNAi.N}

Also Known As

yki^RNAi, UAS-yki-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Zhang et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Zhang et al., 2008)

Mutagen

in vitro construct

(Zhang et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-yki.RNAi.N}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of a fragment encoding yki amino acid residues 1-180.

(Zhang et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

yki

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS

(Waghmare et al., 2024)

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Irwin et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ey.PH

(Zhang et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Zhang et al., 2008)

Detailed Description

Statement

Reference

The eye overgrowth phenotype caused by expression of yki^Scer\UAS.cZa under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of yki^{dsRNA.N.Scer\UAS}.

(Zhang et al., 2008)

Expression of yki^{dsRNA.N.Scer\UAS} under the control of Scer\GAL4^ey.PH (in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) results in a small eye phenotype.

(Zhang et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

yki^RNAi.N.UAS/Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B is a suppressor of increased cell number | somatic clone | heat sensitive phenotype of Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, wts^KK101055

(Ren et al., 2010)

Scer\GAL4^NP0001/yki^RNAi.N.UAS, Scer\GAL80^ts.αTub84B is a suppressor of increased cell number | somatic clone | heat sensitive phenotype of Scer\GAL4^NP0001, Scer\GAL80^ts.αTub84B, wts^KK101055

(Ren et al., 2010)

yki^RNAi.N.UAS/Scer\GAL4^NP5130 is a suppressor of increased cell number | somatic clone phenotype of Scer\GAL4^NP5130, wts^KK101055

(Ren et al., 2010)

yki^RNAi.N.UAS/Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | somatic clone | adult stage phenotype of Scer\GAL4^NP5130, wts^KK101055

(Ren et al., 2010)

Scer\GAL4^NP0001/yki^RNAi.N.UAS is a suppressor of increased occurrence of cell division | somatic clone | adult stage phenotype of Scer\GAL4^NP0001, wts^KK101055

(Ren et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

yki^RNAi.N.UAS/Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B is a suppressor of intestinal stem cell | somatic clone phenotype of Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, wts^KK101055

(Ren et al., 2010)

Scer\GAL4^NP0001/yki^RNAi.N.UAS, Scer\GAL80^ts.αTub84B is a suppressor of intestinal stem cell | somatic clone phenotype of Scer\GAL4^NP0001, Scer\GAL80^ts.αTub84B, wts^KK101055

(Ren et al., 2010)

yki^RNAi.N.UAS/Scer\GAL4^NP5130 is a suppressor of intestinal stem cell | somatic clone phenotype of Scer\GAL4^NP5130, wts^KK101055

(Ren et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expressing yki^{dsRNA.N.Scer\UAS} in hpo^BF33 mutant MARCM clones (using Scer\GAL4^esg-NP5130) reduces the clone size.

Co-expression of yki^{dsRNA.N.Scer\UAS} with wts^KK101055 in adult intestine stem cells and enteroblasts using Scer\GAL4^esg-NP5130 suppresses the increase in the number of precursor cells.

Co-expression of yki^{dsRNA.N.Scer\UAS} in flies driving wts^KK101055 under the control of Scer\GAL80^ts.αTub84B-Scer\GAL4^esg-NP5130 that have been shifted to 29[o]C for 14 days suppresses the dramatic increase seen in the number of intestine stem cells found upon expression of wts^KK101055 alone.

Co-expression of yki^{dsRNA.N.Scer\UAS} with wts^KK101055 in absorptive enterocytes in the adult midgut under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B suppresses the proliferation increase in intestine stem cells seen upon expression of wts^KK101055 alone.

Knock-down of yki through co-expression of yki^{dsRNA.N.Scer\UAS} in upd1^Scer\UAS.cZa MARCM mutant clones (under the control of Scer\GAL4^esg-NP5130) does not affect intestinal stem cell proliferation.

Knock-down of yki through co-expression of yki^{dsRNA.N.Scer\UAS} in Egfr^{1.A887T.Scer\UAS} MARCM mutant clones (under the control of Scer\GAL4^esg-NP5130) does not affect intestinal stem cell proliferation.

(Ren et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

yki^RNAi.N.UAS

yki^RNAi2

(Chen et al., 2020)

yki^{dsRNA.N.Scer\UAS}

yki^dsRNA.N.UAS

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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