FlyBase Allele Report: Dmel\Rho1[RNAi.1.UAS]

General Information

Symbol

Dmel\Rho1^RNAi.1.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0240534

Feature type

allele

Associated gene

Dmel\Rho1

Associated Insertion(s)

Carried in Construct

P{UAS-Rho1.RNAi.1}

Key Links

Transgenic product class

RNAi_reagent

(Warner and Longmore, 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Warner and Longmore, 2009)

Mutagen

in vitro construct

(Warner and Longmore, 2009)

Progenitor genotype

Carried in construct

P{UAS-Rho1.RNAi.1}

Cytology

Description

UAS regulatory regions drive expression of Rho1 inverted repeat coding sequences corresponding to bases 325-786.

(Warner and Longmore, 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rho1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates high grade glioma

modeled by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, btl::Egfr^λ.UAS

(Chen et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adherens junction | pupal stage, with Scer\GAL4^GMR.PF

(Warner and Longmore, 2009)

adherens junction | somatic clone, with Scer\GAL4^Act5C.PI

(Warner and Longmore, 2009)

epithelial cell | pupal stage, with Scer\GAL4^GMR.PF

(Warner and Longmore, 2009)

eye | pupal stage, with Scer\GAL4^GMR.PF

(Warner and Longmore, 2009)

hub cell | somatic clone, with Scer\GAL4^Act5C.PI

(Warner and Longmore, 2009)

Detailed Description

Statement

Reference

Expression of Rho1^{1.dsRNA.Scer\UAS} throughout the pupal eye, beginning at puparium formation (0h APF), under the control of Scer\GAL4^GMR.PF results in severe disruption of the adherens junctions. Only the adherens junctions between the pigment epithelial cells (PECs) are affected, with those between a PEC and a cone cell or between cone cells appearing normal.

Depleting Rho1 in a single pigment epithelial cell through expression of Rho1^{1.dsRNA.Scer\UAS} using the flippase-out technique (under the control of Scer\GAL4^Act5C.PI) does not affect the adherens junctions or the polarised localisation of shg but does result in an enlarged apical cell area. However, in multiple-cell Rho1^{1.dsRNA.Scer\UAS} clones, adherens junctions are disrupted but only between adjacent clonal cells and not between wild-type and clonal cells. An enlarged apical area is present in all Rho1^{1.dsRNA.Scer\UAS}-depleted clones regardless of the Rho1 status of neighboring cells.

Depletion of Rho1 in the pupal wing through expression of Rho1^{1.dsRNA.Scer\UAS} using the flippase-out technique (under the control of Scer\GAL4^Act5C.PI) results in increased apical cell areas and disruption of adherens junctions but not septate junctions.

(Warner and Longmore, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rho1^RNAi.1.UAS, Scer\GAL4^GMR.PFa is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Suppressor of

Statement

Reference

Rho1^RNAi.1.UAS, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Rho1^RNAi.1.UAS, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Rho1^RNAi.1.UAS, Scer\GAL4^Act5C.PI has adherens junction | somatic clone phenotype, non-suppressible by shg^UAS.cSa, Scer\GAL4^Act5C.PI

(Warner and Longmore, 2009)

Enhancer of

Statement

Reference

Rho1^RNAi.1.UAS, Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Suppressor of

Statement

Reference

Rho1^RNAi.1.UAS, Scer\GAL4^repo.PU is a suppressor of larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Rho1^RNAi.1.UAS, Scer\GAL4^repo.PU is a suppressor of larval brain neuropil glial cell | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa is exacerbated further by co-expression of Rho1^{dsRNA.1.Scer\UAS} RNAi resulting in a 'no eye' phenotype with scar-like and necrosis-like tissue present.

(Ruan et al., 2016)

Clones expressing Rho1^{1.dsRNA.Scer\UAS} and overexpressing shg^Scer\UAS.cSa exhibit disrupted adherens junctions.

Expression of Rho1^{1.dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI does not affect the adherens junctions between Cdc42⁴ mutant cells.

Expression of Rho1^{1.dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI does not affect the adherens junctions between par-6^Δ226 mutant cells.

(Warner and Longmore, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rho1^RNAi.1.UAS is rescued by Rho1^RNAi.1.UAS/Scer\GAL4^GMR.PF

(Warner and Longmore, 2009)

Rescues

Rho1^RNAi.1.UAS/Scer\GAL4^GMR.PF rescues Rho1^RNAi.1.UAS

(Warner and Longmore, 2009)

Comments

Co-expression of Rho1^Scer\UAS.cMa with Rho1^{1.dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF rescues pupal eye formation.

(Warner and Longmore, 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29002

w^*; P{UAS-Rho1.RNAi.1}2/T(2;3)SM6b-TM6B, ap^RK Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rho1^{1.dsRNA.Scer\UAS}

Rho1^RNAi.1.UAS

Rho1^{dsRNA.1.Scer\UAS}

Rho1^dsRNA.1.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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