FlyBase Allele Report: Dmel\cdm[177]

General Information

Symbol

Dmel\cdm¹⁷⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0240916

Feature type

allele

Associated gene

Dmel\cdm

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Giagtzoglou et al., 2009)

Mutagen

P-element activity

(Giagtzoglou et al., 2009)

Progenitor genotype

P{RS5}cdm^5-SZ-3929

(Giagtzoglou et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes the majority of the cdm coding sequence.

(Giagtzoglou et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Giagtzoglou et al., 2009)

abnormal neurophysiology | third instar larval stage

(Giagtzoglou et al., 2009)

abnormal neurophysiology | third instar larval stage (with Df(3R)DG2)

(Giagtzoglou et al., 2009)

lethal - all die before end of larval stage (with cdm¹⁶⁴)

(Giagtzoglou et al., 2009)

lethal - all die before end of larval stage (with cdm^FY561)

(Giagtzoglou et al., 2009)

lethal - all die before end of larval stage (with cdm^sd4)

(Giagtzoglou et al., 2009)

lethal - all die before end of larval stage (with cdm^sd6)

(Giagtzoglou et al., 2009)

lethal - all die before end of larval stage (with Df(3R)DG2)

(Giagtzoglou et al., 2009)

lethal - all die before end of larval stage (with Df(3R)Exel6178)

(Giagtzoglou et al., 2009)

lethal - all die before end of larval stage | recessive

(Giagtzoglou et al., 2009)

some die during third instar larval stage (with cdm¹⁶⁴)

(Giagtzoglou et al., 2009)

some die during third instar larval stage (with cdm^FY561)

(Giagtzoglou et al., 2009)

some die during third instar larval stage (with cdm^sd4)

(Giagtzoglou et al., 2009)

some die during third instar larval stage (with cdm^sd6)

(Giagtzoglou et al., 2009)

some die during third instar larval stage (with Df(3R)DG2)

(Giagtzoglou et al., 2009)

some die during third instar larval stage (with Df(3R)Exel6178)

(Giagtzoglou et al., 2009)

some die during third instar larval stage | recessive

(Giagtzoglou et al., 2009)

Phenotype Manifest In

embryonic/larval neuromuscular junction

(Giagtzoglou et al., 2009)

embryonic/larval neuromuscular junction (with Df(3R)DG2)

(Giagtzoglou et al., 2009)

postsynaptic membrane

(Giagtzoglou et al., 2009)

Detailed Description

Statement

Reference

Homozygous clones in the visual system result in defects in electroretinogram recordings; the on and off transients are absent, and the hyperpolarisation curve is dramatically reduced compared to wild type.

cdm¹⁷⁷/Df(3R)DG2 animals derived from females containing cdm¹⁷⁷ homozygous germline clones show third larval instar lethality.

The number of synaptic boutons per muscle area is normal at the third larval instar cdm¹⁷⁷ neuromuscular junction, although there is a small decrease in the total surface area of muscles 6 and 7 compared to controls. The number of active zones and the length of the postsynaptic density are normal. The ratio of subsynaptic reticulum (SSR) per bouton area and the SSR density are reduced compared to controls.

The amplitude and frequency of miniature excitatory junctional potentials at the larval neuromuscular junction is normal in homozygous cdm¹⁷⁷ and cdm¹⁷⁷/Df(3R)DG2 animals.

At 2mM extracellular Ca[2+], the evoked excitatory junctional potential (EJP) at the larval neuromuscular junction is normal in homozygous cdm¹⁷⁷ and cdm¹⁷⁷/Df(3R)DG2 animals. At low concentrations (0.35mM and 0.5mM) extracellular Ca[2+], the amplitude of the evoked EJP at the larval neuromuscular junction is significantly increased in homozygous cdm¹⁷⁷ and cdm¹⁷⁷/Df(3R)DG2 animals compared to controls.

(Giagtzoglou et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions