FlyBase Allele Report: Dmel\Nhe2[1]

General Information

Symbol

Dmel\Nhe2¹

Species

D. melanogaster

Name

FlyBase ID

FBal0241142

Feature type

allele

Associated gene

Dmel\Nhe2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Simons et al., 2009)

Progenitor genotype

P{EPgy2}Nhe2^EY11323

(Simons et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 6.1kb deletion.

(Simons et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:21,102,439..21,108,539 [+]

Comment:

Estimated endpoints of 6.1kb deletion resulting from the imprecise excision of P{EPgy2}Nhe2^EY11323, which removes the promoters and 5' untranslated exons of Nhe2 . One breakpoint is at the insertion site and the other is 6.1 kb upstream.

(Simons et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Nhe2^RNAi.UAS.WIZ, Scer\GAL4^hs.2sev

(Simons et al., 2009)

cell lethal | recessive | somatic clone

(Simons et al., 2009)

lethal - all die before end of larval stage | recessive

(Simons et al., 2009)

some die during larval stage | recessive

(Simons et al., 2009)

Phenotype Manifest In

embryonic/larval fat body

(Simons et al., 2009)

eye, with Nhe2^RNAi.UAS.WIZ, Scer\GAL4^hs.2sev

(Simons et al., 2009)

larval digestive system

(Simons et al., 2009)

ommatidium, with Nhe2^RNAi.UAS.WIZ, Scer\GAL4^hs.2sev

(Simons et al., 2009)

Detailed Description

Statement

Reference

Homozygotes die during early larval stages with defects in gut morphology and function and fat body development.

Homozygous clones can not be recovered in adult or larval tissue.

Expression of Nhe2^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^hs.2sev in a Nhe2¹/+ background results in planar cell polarity defects in the eye, as well as general defects in eye morphology and structure.

(Simons et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Nhe2^RNAi.UAS.WIZ, Nhe2[+], Nhe2¹, Scer\GAL4^hs.2sev is a suppressor | partially of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, fz^UAS.cAa

(Simons et al., 2009)

Phenotype Manifest In

Suppressor of

Statement

Reference

Nhe2^RNAi.UAS.WIZ, Nhe2[+], Nhe2¹, Scer\GAL4^hs.2sev is a suppressor | partially of eye phenotype of Scer\GAL4^hs.2sev, fz^UAS.cAa

(Simons et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The planar cell polarity defects in the eye that are caused by expression of fz^Scer\UAS.cAa under the control of Scer\GAL4^hs.2sev are partially suppressed if the flies also simultaneously co-express Nhe2^{dsRNA.Scer\UAS.WIZ} and carry Nhe2¹/+.

(Simons et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nhe2¹ is rescued by Nhe2^long.UAS/Scer\GAL4^Tub.PU

(Simons et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nhe2¹

(Simons et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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