FlyBase Allele Report: Dmel\Appl[sdΔIC.UAS]

General Information

Symbol

Dmel\Appl^sdΔIC.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0241478

Feature type

allele

Associated gene

Dmel\Appl

Associated Insertion(s)

Carried in Construct

P{UAS-Appl.sdΔIC}

Key Links

Transgenic product class

inframe_deletion

(Torroja et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Torroja et al., 1999)

Mutagen

in vitro construct

(Merdes et al., 2004, Torroja et al., 1999)

Progenitor genotype

Appl^UAS.cTa

(Torroja et al., 1999)

Appl^sd.UAS

(Merdes et al., 2004)

Carried in construct

P{UAS-Appl.sdΔIC}

Cytology

Description

Construct: The whole of the C terminal ICD region has been removed.

(Merdes et al., 2004)

Deletion of the cleavage site (therefore protein tethered to membrane), G_obinding domain and internalization signal (amino acids 836-886) of the Appl coding region.

(Torroja et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Appl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease 1

CEA

(Rieche et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

visible, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

wild-type, with Scer\GAL4^sca-537.4

(Merdes et al., 2004)

Phenotype Manifest In

wing, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

wing pouch, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

wing vein, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Detailed Description

Statement

Reference

Expression of Appl^{sd.ΔIC.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 results in a reduction in wing size and breaks in the wing vein pattern. The wing discs of these animals show a large increase in cell death throughout the wing pouch.

(Kim et al., 2007)

When Appl^Scer\UAS.cTa is driven by Scer\GAL4^sca-537.4 no phenotype is seen.

(Merdes et al., 2004)

When expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) total number of synaptic boutons is unchanged, percentage of satellite boutons is unchanged and the number of normal boutons is unchanged.

(Torroja et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Appl^sdΔIC.UAS, Scer\GAL4^Bx-MS1096 has increased cell death phenotype, enhanceable by APP-BP1[+]/APP-BP1^Ex62

(Kim et al., 2007)

NOT suppressed by

Statement

Reference

Appl^sdΔIC.UAS, Scer\GAL4^Bx-MS1096 has visible phenotype, non-suppressible by APP-BP1^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Appl^sdΔIC.UAS, Scer\GAL4^Bx-MS1096 has increased cell death phenotype, non-suppressible by APP-BP1^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Appl^sdΔIC.UAS, Scer\GAL4^Bx-MS1096 has wing disc phenotype, enhanceable by APP-BP1[+]/APP-BP1^Ex62

(Kim et al., 2007)

NOT Enhanced by

Statement

Reference

Appl^sdΔIC.UAS, Scer\GAL4^Appl.G1a has larval nervous system phenotype, non-enhanceable by Df(3L)GN24

(Gunawardena and Goldstein, 2001)

Appl^sdΔIC.UAS, Scer\GAL4^Appl.G1a has larval nervous system phenotype, non-enhanceable by robl^K

(Gunawardena and Goldstein, 2001)

NOT suppressed by

Statement

Reference

Appl^sdΔIC.UAS, Scer\GAL4^Bx-MS1096 has wing phenotype, non-suppressible by APP-BP1^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Appl^sdΔIC.UAS, Scer\GAL4^Bx-MS1096 has wing pouch phenotype, non-suppressible by APP-BP1^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Appl^sdΔIC.UAS, Scer\GAL4^Appl.G1a has larval nervous system phenotype, non-suppressible by robl^K

(Gunawardena and Goldstein, 2001)

Appl^sdΔIC.UAS, Scer\GAL4^Appl.G1a has larval nervous system phenotype, non-suppressible by Df(3L)GN24

(Gunawardena and Goldstein, 2001)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Appl.G1a/Appl^sdΔIC.UAS is a non-enhancer of larval nervous system phenotype of Klc^8ex94

(Gunawardena and Goldstein, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The wing phenotypes caused by expression of Appl^{sd.ΔIC.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 are not suppressed by co-expression of APP-BP1^Scer\UAS.cKa.

The increased cell death seen in the wing discs of animals expressing Appl^{sd.ΔIC.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 is not suppressed by co-expression of APP-BP1^Scer\UAS.cKa, but is enhanced by APP-BP1^Ex62/+.

(Kim et al., 2007)

The addition of Df(3L)GN24 to Appl^{sdΔC.Scer\UAS}, Scer\GAL4^Appl.G1a larvae has no effect on the larval axons. The addition of robl^K to Appl^{sdΔC.Scer\UAS}, Scer\GAL4^Appl.G1a larvae has no effect on the larval axons.

(Gunawardena and Goldstein, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29865

y¹ w^*; P{UAS-Appl.sdΔIC}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Appl^{sd.ΔIC.Scer\UAS}

Appl^{sdΔC.Scer\UAS}

Appl^{sdΔIC.Scer\UAS}

Appl^sdΔIC.UAS

Name Synonyms

Secondary FlyBase IDs

FBal0188190
FBal0103098

References (8)

Report Sections

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