FlyBase Allele Report: Dmel\Appl[sd.UAS]

General Information

Symbol

Dmel\Appl^sd.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0103093

Feature type

allele

Associated gene

Dmel\Appl

Associated Insertion(s)

Carried in Construct

P{UAS-Appl.sd}

Also Known As

UAS-APPL^SD, APPL^sd

Key Links

Transgenic product class

inframe_deletion

(Torroja et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Torroja et al., 1999)

Mutagen

in vitro construct

(Torroja et al., 1999)

Progenitor genotype

Appl^UAS.cTa

(Torroja et al., 1999)

Carried in construct

P{UAS-Appl.sd}

Cytology

Description

Deletion of the cleavage site (therefore protein tethered to membrane), transmembrane domain, G_obinding domain, internalization signal of the Appl coding region.

(Torroja et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Appl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease 1

CEC

(Penserga et al., 2019)

CEA

(Penserga et al., 2019, Rieche et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain, with Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

axon | adult stage | progressive, with Scer\GAL4^GMR91H05

(Penserga et al., 2019)

bouton | ectopic, with Scer\GAL4^unspecified

(Torroja et al., 1999)

cell body rind of adult lamina, with Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

dense core granule | decreased number | male limited, with Scer\GAL4^dsx.KI

(Singh et al., 2025)

giant fiber neuron | adult stage | progressive, with Scer\GAL4^GMR91H05

(Penserga et al., 2019)

larval ventral nerve cord | third instar larval stage, with Scer\GAL4^Appl.PU

(Wang et al., 2015)

male accessory gland secondary cell, with Scer\GAL4^dsx.KI

(Singh et al., 2025)

mechanosensory sensory organ, with Scer\GAL4^sca-537.4

(Merdes et al., 2004)

NMJ bouton, with Scer\GAL4^C164

(Ashley et al., 2005)

wing, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

wing pouch, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

wing vein, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Detailed Description

Statement

Reference

Expressing Appl^sd.UAS under the control of Scer\GAL4^GMR91H05 results in adult giant fibers presenting morphological and functional defects: there is a severe decrease in the frequency of synaptic response upon high frequency stimulation (10 trains of 10 stimulations at 100 Hz); although 2-3 days old adults present terminals in the synaptic target region (second thoracic neuromere) and without pruning defects, most terminals are stunted or exhibit bulb-like endings; in 14 and 28 days old adults, terminals are progressively seen outside the target area (including second thoracic neuromere, CvC and brain).

(Penserga et al., 2019)

Overexpression of Appl^sd.Scer\UAS for 48 hours, under the control of RU-inducible Scer\GAL4^{Mef2.247.Switch}, does not affect short term memory.

(Bourdet et al., 2015)

Expression of Appl^sd.Scer\UAS driven by Scer\GAL4^Appl.PU induces significant cell death in the ventral nerve cord compared to controls.

(Wang et al., 2015)

28 day old flies expressing Appl^sd.Scer\UAS under the control of Scer\GAL4^GMR.PU show degeneration of the lamina cortex.

(Bolkan et al., 2012)

Flies expressing Appl^sd.Scer\UAS under the control of Scer\GAL4^Appl.G1a show increased vacuolisation in the brain compared to controls. 55% of 5 day old flies contain single vacuoles that are confined to the lamina.

(Wentzell et al., 2012)

Expression of Appl^sd.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 results in a reduction in wing size and breaks in the wing vein pattern. The wing discs of these animals show a large increase in cell death throughout the wing pouch.

(Kim et al., 2007)

Expression of Appl^sd.Scer\UAS pre-synaptically (under the control of Scer\GAL4^C164) results in a dramatic increase in bouton number.

(Ashley et al., 2005)

When Appl^sd.Scer\UAS is driven by Scer\GAL4^sca-537.4 a strong bristle transformation phenotype is seen.

(Merdes et al., 2004)

When expression is driven in the motoneurons extra small boutons of abnormal morphology develop. Many small satellite boutons bud off from a central bouton of normal appearance. Molecular markers suggest that the extra boutons are sufficiently normal to form functional synapses. When expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) total number of synaptic boutons is increased, percentage of satellite boutons is increased and the number of normal boutons is increased. When Appl^sd.Scer\UAS expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) in combination with Appl^d total number of synaptic boutons is increased, percentage of satellite boutons is increased and the number of normal boutons is increased.

(Torroja et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Appl^sd.UAS, Scer\GAL4^Bx-MS1096 has increased cell death phenotype, enhanceable by APP-BP1[+]/APP-BP1^Ex62

(Kim et al., 2007)

Suppressed by

Statement

Reference

Appl^sd.UAS, Scer\GAL4^Bx-MS1096 has visible phenotype, suppressible by APP-BP1^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Appl^sd.UAS, Scer\GAL4^Bx-MS1096 has increased cell death phenotype, suppressible by APP-BP1^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Enhancer of

Statement

Reference

Appl^sd.UAS, Scer\GAL4^GMR.PU is an enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of Bace^GD5366, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Scer\GAL4^Appl.G1a/Appl^sd.UAS is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Appl^sd.UAS, Scer\GAL4^Bx-MS1096 has wing disc phenotype, enhanceable by APP-BP1[+]/APP-BP1^Ex62

(Kim et al., 2007)

Appl^sd.UAS, Scer\GAL4^futsch-C380 has NMJ bouton phenotype, enhanceable by Fas2^UAS.cAa/Scer\GAL4^BG487

(Ashley et al., 2005)

Suppressed by

Statement

Reference

Appl^sd.UAS, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible by APP-BP1^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Appl^sd.UAS, Scer\GAL4^Bx-MS1096 has wing pouch phenotype, suppressible by APP-BP1^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Appl^sd.UAS, Scer\GAL4^C164 has NMJ bouton phenotype, suppressible by Fas2[+]/Fas2^e76, Scer\GAL4^C164

(Ashley et al., 2005)

Appl^sd.UAS, Scer\GAL4^Appl.G1a has larval nervous system phenotype, suppressible by robl^K

(Gunawardena and Goldstein, 2001)

Enhancer of

Statement

Reference

Appl^sd.UAS, Scer\GAL4^GMR.PU is an enhancer of cell body rind of adult lamina | progressive phenotype of Bace^GD5366, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Scer\GAL4^Appl.G1a/Appl^sd.UAS is an enhancer of larval brain | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

Scer\GAL4^futsch-C380/Appl^sd.UAS is an enhancer of NMJ bouton phenotype of Fas2^UAS.cAa, Scer\GAL4^BG487

(Ashley et al., 2005)

Scer\GAL4^Appl.G1a/Appl^sd.UAS is an enhancer of larval nervous system phenotype of Khc⁸

(Gunawardena and Goldstein, 2001)

Other

Statement

Reference

Appl^sd.UAS, Scer\GAL4^unspecified, Sh¹⁴, eag¹ has bouton phenotype

(Torroja et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Appl^sd.Scer\UAS under the control of Scer\GAL4^Appl.G1a significantly enhances the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

(Wentzell et al., 2012)

The wing phenotypes caused by expression of Appl^sd.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 are suppressed by co-expression of APP-BP1^Scer\UAS.cKa.

The increased cell death seen in the wing discs of animals expressing Appl^sd.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 is suppressed by co-expression of APP-BP1^Scer\UAS.cKa, and is enhanced by APP-BP1^Ex62/+.

(Kim et al., 2007)

A Fas2^e76 background suppresses the increase in bouton number observed when Appl^sd.Scer\UAS is overexpressed pre-synaptically (under the control of Scer\GAL4^C164).

Overexpression of both Appl^sd.Scer\UAS and Fas2^Scer\UAS.cAa both pre- and post-synaptically (under the control of Scer\GAL4^C380 and Scer\GAL4^BG487) increase the number of boutons by >400%.

(Ashley et al., 2005)

The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4^Appl.G1a) is suppressed by the addition of robl^K/+.

(Gunawardena and Goldstein, 2001)

When Appl^sd.Scer\UAS expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) in combination with eag¹, Sh¹⁴ total number of synaptic boutons is decreased compared to Appl^sd.Scer\UAS alone, percentage of satellite boutons is decreased compared to Appl^sd.Scer\UAS alone and the number of normal boutons is increased compared to Appl^sd.Scer\UAS alone.

(Torroja et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{Mef2.247.Switch}/Appl^sd.UAS rescues Appl^d

(Bourdet et al., 2015)

Fails to rescue

Scer\GAL4^{Mef2.247.Switch}/Appl^sd.UAS fails to rescue Appl^d

(Bourdet et al., 2015)

Comments

Expression of Appl^sd.Scer\UAS in RU-fed flies suppresses the short-term memory defect found in Appl^d/+; Scer\GAL4^{Mef2.247.Switch} flies. In contrast, in the absence of RU-feeding, Appl^d/+; Scer\GAL4^{Mef2.247.Switch}/Appl^sd.Scer\UAS flies exhibit scores that are indistinguishable from controls and significantly lower than that found in wild-type flies.

RU-induced expression of Appl^sd.Scer\UAS under the control of Scer\GAL4^{Mef2.247.Switch} fails to rescue the long term memory deficit caused by Appl^d/+ partial loss-of-function.

(Bourdet et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29863

w¹¹¹⁸; P{UAS-Appl.sd}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Appl^sd.Scer\UAS

Appl^sd.UAS

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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