FlyBase Allele Report: Dmel\Tbce[UAS.cJa]

General Information

Symbol

Dmel\Tbce^UAS.cJa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Jin

FlyBase ID

FBal0241481

Feature type

allele

Associated gene

Dmel\Tbce

Associated Insertion(s)

Carried in Construct

P{UAS-tbce.J}

Also Known As

UAS-TBCE

Key Links

Transgenic product class

cDNA

(Jin et al., 2009)

wild_type

(Jin et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Jin et al., 2009)

wild_type

(Jin et al., 2009)

Mutagen

in vitro construct

(Jin et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-tbce.J}

Cytology

Description

UASt regulatory sequences drive expression of the full-length Tbce GM13256 cDNA clone.

(Jin et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tbce

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage, with Scer\GAL4^C57

(Jin et al., 2009)

abnormal locomotor behavior | larval stage, with Scer\GAL4^elav-C155

(Jin et al., 2009)

abnormal neuroanatomy | larval stage, with Scer\GAL4^C57

(Jin et al., 2009)

Phenotype Manifest In

embryonic/larval neuromuscular junction, with Scer\GAL4^C57

(Jin et al., 2009)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^RapGAP1-OK6

(Saunders et al., 2022)

microtubule, with Scer\GAL4^C57

(Jin et al., 2009)

microtubule & neuromuscular junction, with Scer\GAL4^elav-C155

(Jin et al., 2009)

NMJ bouton, with Scer\GAL4^C57

(Jin et al., 2009)

type Ib terminal bouton | increased number | larval stage, with Scer\GAL4^RapGAP1-OK6

(Saunders et al., 2022)

Detailed Description

Statement

Reference

DL1 olfactory projection neuron clones expressing Tbce^Scer\UAS.cJa under the control of Scer\GAL4^GH146 do not exhibit defects in dendrite or axon morphology.

(Okumura et al., 2015)

Larvae expressing tbce^Scer\UAS.cJa under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^C57 show normal rhythmic peristalsis and crawling activity. However, the larvae perform less well in a roll-over assay than control flies, having a significantly increased roll-over time.

Expression of tbce^Scer\UAS.cJa under the control of Scer\GAL4^elav-C155 has no effect on synaptic branching, bouton number or average bouton area at the third larval instar neuromuscular junction (NMJ).

Expression of tbce^Scer\UAS.cJa under the control of Scer\GAL4^C57 results in an increase in synaptic branching and bouton number and a decrease in average bouton area at the third larval instar NMJ compared to controls.

Expression of tbce^Scer\UAS.cJa under the control of Scer\GAL4^elav-C155 results in an increase in the amplitude of excitatory junction potentials (EJPs) and in the amplitude miniature EJPs (mEJPs) at the NMJ compared to controls. The frequency of mEJPS is normal. Quantal content is elevated.

Expression of tbce^Scer\UAS.cJa under the control of Scer\GAL4^C57 has no effect on the amplitude of excitatory junction potentials (EJPs) or the amplitude or frequency of miniature EJPs (mEJPs) at the NMJ compared to controls. Quantal content is normal.

Expression of tbce^Scer\UAS.cJa under the control of Scer\GAL4^elav-C155 results in a continuous and smooth microtubule cytoskeleton extending to the very tip of the NMJ synaptic terminal.

Expression of tbce^Scer\UAS.cJa under the control of Scer\GAL4^C57 increases the microtubule network in muscles, with a prominent perinuclear enrichment.

(Jin et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^C57, Tbce^UAS.cJa has microtubule phenotype, suppressible | partially by Scer\GAL4^C57

(Jin et al., 2009)

Suppressor of

Statement

Reference

Tbce^UAS.cJa/Scer\GAL4^RapGAP1-OK6 is a suppressor of type Ib terminal bouton | increased number | larval stage phenotype of αTub84B^K394R

(Saunders et al., 2022)

Tbce^UAS.cJa/Scer\GAL4^RapGAP1-OK6 is a suppressor of microtubule | larval stage phenotype of αTub84B^K394R

(Saunders et al., 2022)

Tbce^UAS.cJa/Scer\GAL4^RapGAP1-OK6 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of αTub84B^K394R

(Saunders et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

When tbce^Scer\UAS.cJa and spas^Scer\UAS.cTa are co-expressed under the control of Scer\GAL4^C57, the microtubule phenotype in the muscles is similar to that of animals expressing spas^Scer\UAS.cTa alone under the control of Scer\GAL4^C57.

spas^5.75 partly suppresses the microtubule phenotype seen in the muscles of animals expressing tbce^Scer\UAS.cJa under the control of Scer\GAL4^C57.

(Jin et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34536

w¹¹¹⁸; P{UAS-tbce.J}3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Tbce^Scer\UAS.cJa

Tbce^UAS.cJa

tbce^Scer\UAS.cJa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Jin

Secondary FlyBase IDs

References (6)

Report Sections

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