FlyBase Allele Report: Dmel\Atg16[MI00187]

General Information

Symbol

Dmel\Atg16^MI00187

Species

D. melanogaster

Name

FlyBase ID

FBal0242831

Feature type

allele

Associated gene

Dmel\Atg16

Associated Insertion(s)

Mi{MIC}Atg16^MI00187

Carried in Construct

Also Known As

Atg16^MI

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{MIC}Atg16^MI00187

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Also carries

integrase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:29,828,119..29,828,119 [+]

(Vincze et al., 2026, Szabó et al., 2023, Bellen, 2010.3.31)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of inflammatory bowel disease 10

CEC

(Nagy et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of inflammatory bowel disease 10

is ameliorated by Atg16^{ΔAutD+Linker.UAS}

(Nagy et al., 2017)

is ameliorated by robo2^EP2582

(Nagy et al., 2017)

is ameliorated by sli^EY10695

(Nagy et al., 2017)

ameliorates cancer

modeled by Raf^F22.hs

(Zhao et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: "inflammatory bowel disease 10" is associated with human ortholog ATG12L1.

(Nagy et al., 2017)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | adult stage

(Nagy et al., 2017)

abnormal immune response | adult stage

(Nagy et al., 2017)

abnormal size | adult stage

(Nagy et al., 2017)

abnormal wound healing | adult stage

(Szabó et al., 2023)

abnormal wound healing | adult stage (with Atg16^d67)

(Szabó et al., 2023)

short lived | conditional

(Szabó et al., 2023)

Phenotype Manifest In

Detailed Description

Statement

Reference

Atg16^MI00187 homozygous adult guts show severe morphological changes, including significant increases in length and diameter accompanied by a significantly thicker epithelium, as compared to controls; this mutant epithelium shows a significant increase in the proportion of pre-enteroendocrine cells and a reciprocal significant decrease in the proportion of mature enteroendocrine cells, associated with a significant delay in pre-enteroendocrine cell maturation and a significant increase in the overall number of Prospero-positive precursor/mature enteroendocrine cells, but shows no changes in the thickness of the peritrophic membrane, in the proportion of mitotic or apoptotic cells or in cell adhesion, as compared to controls.

Atg16^MI00187 homozygous adults show a severe decrease in lifespan and a significant increase in the gut bacteria burden upon oral infection with Pseudomonas aeruginosa or Enterococcus faecalis, as compared to control; in the absence of infection, these individuals show a significant increase in the gut burden of Enterobacteriaceae (but not in the overall burden of bacteria, Lactobacilli or Acetobacteriaceae), as compared to controls. These mutants do not show significant changes in food uptake.

(Nagy et al., 2017)

Atg16^MI00187/Atg16^MI00187 mutants have similar spermiogenesis to wild type, with spermatid nuclei showing wild-type morphology.

(Eren-Ghiani et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg16^MI00187 has abnormal cell number | adult stage phenotype, suppressible | partially by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 has abnormal cell number | adult stage phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 has abnormal immune response | adult stage phenotype, suppressible by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 has abnormal immune response | adult stage phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg16^MI00187 has adult posterior midgut epithelium phenotype, suppressible by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 has enteroendocrine cell | adult stage phenotype, suppressible by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 has enteroblast | adult stage phenotype, suppressible by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 has adult posterior midgut epithelium phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 has enteroendocrine cell | adult stage phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 has enteroblast | adult stage phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Both the increased proportion of pre-enteroendocrine cells and the reciprocal decreased proportion of mature enteroendocrine cells observed in the adult gut of Atg16^MI00187 are fully suppressed by the expression of robo2^EP2582 or sli^EY10695 under the control of Scer\GAL4^esg.PU; their severe decrease in longevity upon oral infection with Pseudomonas aeruginosa or Enterococcus faecalis is also suppressed by the expression of sli^EY10695 or robo2^EP2582 under the control of Scer\GAL4^esg.PU.

(Nagy et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Atg16^MI00187 is rescued by Atg16^{UAS.c.Tag:FLAG}/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 is rescued by Atg16^ΔAutD.UAS/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^MI00187 is rescued by Atg16^{ΔAutD+Linker.UAS}/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Not rescued by

Atg16^MI00187 is not rescued by Atg16^ΔWD40.UAS/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Comments

Both the increased proportion of pre-enteroendocrine cells and the reciprocal decreased proportion of mature enteroendocrine cells observed in the adult gut of Atg16^MI00187 are fully suppressed by the expression of Atg16^{Scer\UAS.c.T:Zzzz\FLAG}, Atg16^{ΔAutD.Scer\UAS} or Atg16^{ΔAutD+Linker.Scer\UAS}, but not Atg16^{ΔWD40.Scer\UAS}, under the control of Scer\GAL4^esg.PU.

The severe decrease in longevity of Atg16^MI00187 homozygotes upon oral infection with Pseudomonas aeruginosa or Enterococcus faecalis is suppressed by the expression of Atg16^{ΔAutD+Linker.Scer\UAS} under the control of Scer\GAL4^esg.PU.

(Nagy et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

30656

y¹ w^*; Mi{MIC}Atg16^MI00187

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Atg16^MI00187

(Vincze et al., 2026, Szabó et al., 2023, Nagy et al., 2017, Eren-Ghiani et al., 2015)

Atg16^MI187

(Szabó et al., 2023)

Atg16^MI

(Vincze et al., 2026, Szabó et al., 2023, Nagy et al., 2017)

CG31033^MI00187

(Bellen, 2010.3.31)

atg16^MI00187

(Zhao et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close