FlyBase Allele Report: Scer\GAL4[GMR.APP]

General Information

Symbol

Scer\GAL4^GMR.APP

Species

S. cerevisiae

Name

FlyBase ID

FBal0244416

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GMR-APP-GAL4}

Also Known As

GMR-APP-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Guo et al., 2003)

Progenitor genotype

Carried in construct

P{GMR-APP-GAL4}

Cytology

Description

GMR regulatory sequences drive expression of a γ-secretase reporter. The reporter consists of a cleavable signal sequence, followed by a C-terminal fragment of Hsap\APP that starts immediately downstream of the β-cleavage site (residue 671) and that also lacks a stop codon. The GAL4 coding region is fused to the C-terminus of the Hsap\APP sequences. In the absence of γ-secretase, the GAL4 coding sequence is tethered at the membrane by the Hsap\APP sequences. In the presence of γ-secretase, cleavage of the Hsap\APP protein sequence releases from the membrane a fragment consisting of the intracellular domain of Hsap\APP and the GAL4 coding region, allowing expression of UAS-containing constructs driven by the GAL4 driver.

(Guo et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Scer\GAL4

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

eye disc posterior to the morphogenetic furrow

(Guo et al., 2003)

Additional Information

Statement

Reference

ScerGAL4^GMR.APP drives expression in presumptive photoreceptor cells in the eye disc; the ScerGAL4 protein is tethered to the cell membrane by HsapAPP, and cannot enter the nucleus to drive expression until cleaved by gamma-secretase.

(Guo et al., 2003)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased size | adult stage, with grim^UAS.cYa

(Nagel et al., 2021)

increased cell death, with grim^UAS.cYa

(Gross et al., 2008)

visible, with grim^UAS.cYa

(Herranz et al., 2006, Guo et al., 2003)

visible | adult stage, with grim^UAS.cYa

(Nagel et al., 2021)

Phenotype Manifest In

eye, with grim^UAS.cYa

(Nagel et al., 2021, Gross et al., 2008, Herranz et al., 2006, Guo et al., 2003)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible | adult stage phenotype, enhanceable by Mnr^EP555, Scer\GAL4^GMR.APP

(Nagel et al., 2021)

Scer\GAL4^GMR.APP, grim^UAS.cYa has decreased size | adult stage phenotype, enhanceable by Mnr^EP555, Scer\GAL4^GMR.APP

(Nagel et al., 2021)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, enhanceable by crb^M11.M2

(Herranz et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible | adult stage phenotype, suppressible by Mnr^GD9327, Scer\GAL4^GMR.APP

(Nagel et al., 2021)

Scer\GAL4^GMR.APP, grim^UAS.cYa has decreased size | adult stage phenotype, suppressible by Mnr^GD9327, Scer\GAL4^GMR.APP

(Nagel et al., 2021)

Scer\GAL4^GMR.APP, grim^UAS.cYa has increased cell death phenotype, suppressible by X11L^GMR.cGa

(Gross et al., 2008)

Scer\GAL4^GMR.APP, grim^UAS.cYa has increased cell death phenotype, suppressible by Hsap\APBA2^GMR.cGa

(Gross et al., 2008)

Scer\GAL4^GMR.APP, grim^UAS.cYa has increased cell death phenotype, suppressible by Hsap\APP^GMR.Tag:MYC

(Gross et al., 2008)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by Diap1^GMR.PH

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by Psn^C1/Psn[+]

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by Psn^D278A.GMR

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by nct[+]/Nct^J2

(Guo et al., 2003)

FBal0366230:, Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by Nct^{RNAi.291-696.GMR}/Nct^{RNAi.291-696.GMR}

(Guo et al., 2003)

FBal0366231:, Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by aph-1^{RNAi.29-174.GMR}/aph-1^{RNAi.29-174.GMR}

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by Df(2L)3548/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by Df(2L)1581/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by Df(2L)90/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by Df(2L)3910/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by Df(2L)1567/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by +/Df(2R)1547

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, suppressible by BacA\p35^GMR.PH

(Guo et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.APP, grim^UAS.cYa has increased cell death phenotype, non-suppressible by X11L^UAS.cGa/Scer\GAL4^GMR.PF

(Gross et al., 2008)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, non-suppressible by Df(2L)97/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, non-suppressible by Df(2L)4954/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has visible phenotype, non-suppressible by Df(2L)6875/+

(Guo et al., 2003)

Enhancer of

Statement

Reference

Mnr^EP555, Scer\GAL4^GMR.APP is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.APP, grim^UAS.cYa

(Nagel et al., 2021)

Mnr^EP555, Scer\GAL4^GMR.APP is an enhancer of decreased size | adult stage phenotype of Scer\GAL4^GMR.APP, grim^UAS.cYa

(Nagel et al., 2021)

Suppressor of

Statement

Reference

Mnr^GD9327, Scer\GAL4^GMR.APP is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.APP, grim^UAS.cYa

(Nagel et al., 2021)

Mnr^GD9327, Scer\GAL4^GMR.APP is a suppressor of decreased size | adult stage phenotype of Scer\GAL4^GMR.APP, grim^UAS.cYa

(Nagel et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, enhanceable by Mnr^EP555, Scer\GAL4^GMR.APP

(Nagel et al., 2021)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, enhanceable by crb^M11.M2

(Herranz et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Mnr^GD9327, Scer\GAL4^GMR.APP

(Nagel et al., 2021)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by X11L^GMR.cGa

(Gross et al., 2008)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Hsap\APBA2^GMR.cGa

(Gross et al., 2008)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Hsap\APP^GMR.Tag:MYC

(Gross et al., 2008)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Psn^D278A.GMR

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by nct[+]/Nct^J2

(Guo et al., 2003)

FBal0366230:, Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Nct^{RNAi.291-696.GMR}/Nct^{RNAi.291-696.GMR}

(Guo et al., 2003)

FBal0366231:, Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by aph-1^{RNAi.29-174.GMR}/aph-1^{RNAi.29-174.GMR}

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Df(2L)3548/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Df(2L)1581/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Df(2L)90/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Df(2L)3910/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Df(2L)1567/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by +/Df(2R)1547

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by BacA\p35^GMR.PH

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Diap1^GMR.PH

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, suppressible by Psn^C1/Psn[+]

(Guo et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, non-suppressible by X11L^UAS.cGa/Scer\GAL4^GMR.PF

(Gross et al., 2008)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, non-suppressible by Df(2L)97/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, non-suppressible by Df(2L)4954/+

(Guo et al., 2003)

Scer\GAL4^GMR.APP, grim^UAS.cYa has eye phenotype, non-suppressible by Df(2L)6875/+

(Guo et al., 2003)

Enhancer of

Statement

Reference

Mnr^EP555, Scer\GAL4^GMR.APP is an enhancer of eye phenotype of Scer\GAL4^GMR.APP, grim^UAS.cYa

(Nagel et al., 2021)

Suppressor of

Statement

Reference

Mnr^GD9327, Scer\GAL4^GMR.APP is a suppressor of eye phenotype of Scer\GAL4^GMR.APP, grim^UAS.cYa

(Nagel et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

52016

P{GMR-APP-GAL4}2, P{UAS-grim.Y}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^GMR.APP

Scer\GAL4^{GMR.T:Hsap\APP}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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