Scer\GAL4NP5288 drives expression in around 65 uniglomerular and 3 multiglomerular antennal lobe projection neurons. The cell bodies of around 36 of these neurons are positioned in the anterior-dorsal part of the antennal lobe. Around 35 cell bodies are found in the lateral part and 7 to 8 are positioned ventrally. The uniglomerular neurons project to a large number of antennal lobe glomeruli including VM7d, DA4m, VP1 and VP4 while he multiglomerular neurons project to all glomeruli.
Scer\GAL4NP5288 is expressed in a subset of neurons in the adult brain, including projection neurons and the mushroom body anterior paired lateral cell. Use of GFP-reconstitution across synaptic partners (GRASP) reveals that there are close contacts between the terminals of the mushroom body dorsal and anterior paired medial cells in the mushroom body alpha'/beta' lobes and in the pedunculus.