FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\P{GawB}NP5288
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General Information
Symbol
Dmel\P{GawB}NP5288
Species
D. melanogaster
Name
FlyBase ID
FBti0036503
Feature type
transposable_element_insertion_site
Also Known As
NP5288, NP5288-GAL4
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
L, Scer\GAL4
Viability / fertility
Associated allele(s)
L5Db1, L5Db2, L5Db3, L5Db4, L5Db5, Scer\GAL4L-5Db5, Scer\GAL4NP5288
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2R ( 51A4 )
Sequence Location
2R:14,458,195..14,458,453 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:14,458,195..14,458,453 [+]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
51A4 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG217381
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
L
(Son and Choi, 2020)
Scer\GAL4
(Pitman et al., 2011, Pauls et al., 2010, Tanaka et al., 2008)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult antennal lobe projection neuron
(Tanaka et al., 2008)
adult mushroom body anterior paired lateral cell
(Flybrain Neuron Database et al., 2018.6.28, Pitman et al., 2011, Tanaka et al., 2008)
adult mushroom body alpha-lobe
(Tanaka et al., 2008)
adult mushroom body beta-lobe
(Tanaka et al., 2008)
pedunculus of adult mushroom body
(Tanaka et al., 2008)
calyx of adult mushroom body
(Tanaka et al., 2008)
adult mushroom body dorsal accessory calyx
(Tanaka et al., 2008)
adult medial antennal lobe tract
(Tanaka et al., 2008)
projection neuron | subset
(Pitman et al., 2011)
adult brain | restricted
(Pitman et al., 2011)
adult mushroom body gamma-lobe
(Tanaka et al., 2008)
adult mushroom body beta'-lobe
(Tanaka et al., 2008)
adult mushroom body alpha'-lobe
(Tanaka et al., 2008)
unilateral antennal lobe projection neuron | subset
(Tanaka et al., 2012)
adult antennal lobe projection neuron D adPN
(Israel et al., 2022, Tanaka et al., 2012)
adult antennal lobe projection neuron DA2 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DL1 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DL4 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DL5 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DM1 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DM2 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DM3 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DM4 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DM5 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DM6 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VA2 adPN
(Israel et al., 2022, Tanaka et al., 2012)
adult antennal lobe projection neuron VA3 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VA4 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VA6 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VM2 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VM3 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VM5d adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VM5v adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VA7l adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VC1 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VC2 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VM7v adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DA1 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DA3 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DA4l adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DC3 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DL3 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VA1d adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VA1v adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DC2 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VA7m lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VL2a adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VL2p lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VM1 lPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron VM4 adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DP1m adPN
(Tanaka et al., 2012)
adult antennal lobe projection neuron DL2v adPN
(Tanaka et al., 2012)
medial antennal lobe tract projection neuron 4 | subset
(Tanaka et al., 2012)
adult neuron | subset of adult ventral nerve cord
(Israel et al., 2022)
uniglomerular antennal lobe projection neuron | subset
(Israel et al., 2022)
adult antennal lobe projection neuron VA5 lPN
(Israel et al., 2022)
adult neuron | subset of adult subesophageal zone
(Israel et al., 2022)
Additional Information
Statement
Reference

ScerGAL4NP5288 drives expression in around 65 uniglomerular and 3 multiglomerular antennal lobe projection neurons. The cell bodies of around 36 of these neurons are positioned in the anterior-dorsal part of the antennal lobe. Around 35 cell bodies are found in the lateral part and 7 to 8 are positioned ventrally. The uniglomerular neurons project to a large number of antennal lobe glomeruli including VM7d, DA4m, VP1 and VP4 while he multiglomerular neurons project to all glomeruli.

(Tanaka et al., 2012)

ScerGAL4NP5288 is expressed in a subset of neurons in the adult brain, including projection neurons and the mushroom body anterior paired lateral cell. Use of GFP-reconstitution across synaptic partners (GRASP) reveals that there are close contacts between the terminals of the mushroom body dorsal and anterior paired medial cells in the mushroom body alpha'/beta' lobes and in the pedunculus.

(Pitman et al., 2011)
 
Marker for
adult antennal lobe projection neuron
(Tanaka et al., 2008)
Reflects expression of
Reporter construct used in assay
Mmus\Cd8aUAS.cLa.EGFP
(Tanaka et al., 2012, Pitman et al., 2011, Tanaka et al., 2008)
Avic\GFPS65T.UAS
(Tanaka et al., 2008)
Avic\GFPspGFP1-10.UAS.Tag:M(hCD4)
(Pitman et al., 2011)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2R:9973056-9973316 determined by FlyBase alignment of dbGSS accession AG217381 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (7)
References (14)