FlyBase Allele Report: Dmel\magu[UAS.Tag:V5]

General Information

Symbol

Dmel\magu^UAS.Tag:V5

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Vuilleumier

FlyBase ID

FBal0245302

Feature type

allele

Associated gene

Dmel\magu

Associated Insertion(s)

Carried in Construct

P{UAS-magu.V5}

Key Links

Transgenic product class

wild_type

(Vuilleumier et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Vuilleumier et al., 2010)

Mutagen

in vitro construct

(Vuilleumier et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-magu.V5}

Cytology

Description

UAS regulatory sequences drive expression of magu. A Tag:V5 tag has been inserted into the coding sequence, in between the signal peptide and the Kazal-domain.

(Vuilleumier et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

magu

Tagged with

Tag:V5

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death | larval stage, with Scer\GAL4^C-765

(Merino et al., 2022)

Phenotype Manifest In

wing blade, with Scer\GAL4^dpp.blk1

(Vuilleumier et al., 2010)

wing disc | larval stage, with Scer\GAL4^C-765

(Merino et al., 2022)

Detailed Description

Statement

Reference

Expression of magu^Scer\UAS.cVa driven by Scer\GAL4^dpp.blk1 results in marked enlargement of the lateral regions of the wing blade at the expense of medial domains.

(Vuilleumier et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^C-765/magu^UAS.Tag:V5 is a non-enhancer of increased cell death | larval stage | recessive phenotype of fwe^ΔExtra.EGFP

(Merino et al., 2022)

Scer\GAL4^C-765/magu^UAS.Tag:V5 is a non-enhancer of increased cell death | larval stage | dominant phenotype of dally^gem

(Merino et al., 2022)

NOT Suppressor of

Statement

Reference

Scer\GAL4^C-765/magu^UAS.Tag:V5 is a non-suppressor of increased cell death | recessive | larval stage phenotype of fwe^ΔExtra.EGFP

(Merino et al., 2022)

Scer\GAL4^C-765/magu^UAS.Tag:V5 is a non-suppressor of increased cell death | dominant | larval stage phenotype of dally^gem

(Merino et al., 2022)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^C-765/magu^UAS.Tag:V5 is a non-enhancer of wing disc | larval stage phenotype of fwe^ΔExtra.EGFP

(Merino et al., 2022)

Scer\GAL4^C-765/magu^UAS.Tag:V5 is a non-enhancer of wing disc | larval stage phenotype of dally^gem

(Merino et al., 2022)

NOT Suppressor of

Statement

Reference

Scer\GAL4^C-765/magu^UAS.Tag:V5 is a non-suppressor of wing disc | larval stage phenotype of fwe^ΔExtra.EGFP

(Merino et al., 2022)

Scer\GAL4^C-765/magu^UAS.Tag:V5 is a non-suppressor of wing disc | larval stage phenotype of dally^gem

(Merino et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

magu^UAS.Tag:V5/Scer\GAL4^upd1.PU rescues magu^e00439/magu^f02256

(Zheng et al., 2011)

Partially rescues

Scer\GAL4^unspecified/magu^UAS.Tag:V5 partially rescues magu²

(Vuilleumier et al., 2010)

Comments

Expression of magu^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^upd in magu^e00439/magu^f02256 flies aged at 29[o]C partially rescues the number of germline stem cells. The fraction of testes with germline stem cells increases to more than 50% and sometimes approaches or equals 100%. Partial rescue occurs in both flies aged 3 days and 12 days. A similar rescue is displayed for the wing vein phenotype.

(Zheng et al., 2011)

The wing patterning phenotype of magu² mutants is restored when magu^Scer\UAS.cVa expression is restricted to any compartment or region of the wing disc. However, 'misplacing' the source of magu^Scer\UAS.cVa results in wings with abnormal sizes and altered proportions.

(Vuilleumier et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

magu^{Scer\UAS.T:SV5\V5}

magu^Scer\UAS.cVa

magu^UAS.Tag:V5

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Vuilleumier

Secondary FlyBase IDs

References (4)

Report Sections

Open Close