The addition of wgl-17 to dallygem/dally06464 flies, leads to enhancement of the posterior supraalar bristle (pSA) phenotype seen in dallygem/dally06464 flies alone: 30% of flies have the phenotype, rather than 1%. The addition of put10460 to dallygem/dally06464 flies, leads to enhancement of the anterior dorsocentral bristle (aDC) phenotype seen in dallygem/dally06464 flies alone: 90% of flies have the phenotype, rather than 24%. The pSA bristle phenotype is also enhanced by the addition of put10460. All flies have the phenotype, rather than 86%. The addition of dallygem/+ enhances the pSA phenotype of Tp(3;3)iro-1 or Df(3L)iro-DFM3/+ flies.
Heterozygosity for wgl-12 increases the penetrance of cuticle defects in dallyΔP-188/dallygem embryos from 16% to 41%. Embryos heterozygous for dallygem derived from mothers heterozygous for sglN71 show cuticle defects characteristic of a reduction in wg signalling. The abnormal genitalia phenotype of dallyΔP-188/dallygem flies is dominantly suppressed (down to less than 5% penetrance) by wgl-12. The abnormal genitalia phenotype of dally06464/dallygem flies is dominantly suppressed (down to less than 10% penetrance) by wgl-17.
Expression of dallyΔGAG.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4A9 restores the L5 wing vein in 35% of dallygem homozygotes and 76% of dallygem/dallyΔP-527 transheterozygotes. It performs a slight rescue of the number of chemosensory bristles in dallygem/dallyΔP-527 flies but does not restore posterior supraalar bristles.
Expression of dallyΔGAG.Scer\UAS under the control of Scer\GAL4A9 partially rescues the phenotypes of dallygem, dallygem/Df(3L)Scf-R6, and dallygem/dallyΔP-527 mutants. The transgene substantially restores chemosensory bristle numbers in the wing margin, almost fully rescues the formation of wing vein L5, almost fully restores the formation of the anterior dorsocentral bristles on the notum without inducing the formation of extra ones for all mutants. However, it fails to restore formation of the posterior supraalar bristles.