FlyBase Allele Report: Dmel\dlp[ΔGAG]

General Information

Symbol

Dmel\dlp^ΔGAG

Species

D. melanogaster

Name

FlyBase ID

FBal0399139

Feature type

allele

Associated gene

Dmel\dlp

Associated Insertion(s)

TI{TI}dlp^ΔGAG

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

loss of function allele

(Nakato et al., 2024)

Mutagen

CRISPR/Cas9

(Nakato et al., 2024)

Progenitor genotype

Associated Insertion(s)

TI{TI}dlp^ΔGAG

Cytology

Description

Five glycosaminoglycan (GAG)-attachment serine residues (at positions 625, 629, 631, 643, and 686) have been substituted for alanines in endogenous dlp.

(Nakato et al., 2024)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

MNV

3L:14,670,193..14,670,194 [+]

Nucleotide change:

AG14670193GC

Amino acid change:

S625A | dlp-PA; S624A | dlp-PC

Reported amino acid change:

S625A

Comment:

First of five glycosaminoglycan (GAG)-attachment serine residues in dlp that have been replaced with Ala. Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Nakato et al., 2024)

MNV

3L:14,670,376..14,670,377 [+]

Nucleotide change:

AG14670376GC

Amino acid change:

S686A | dlp-PA; S685A | dlp-PC

Reported amino acid change:

S686A

Comment:

Fifth of five glycosaminoglycan (GAG)-attachment serine residues in dlp that have been replaced with Ala. Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Nakato et al., 2024)

point_mutation

3L:14,670,205..14,670,205 [+]

Nucleotide change:

T14670205G

Amino acid change:

S629A | dlp-PA; S628A | dlp-PC

Reported amino acid change:

S629A

Comment:

Second of five glycosaminoglycan (GAG)-attachment serine residues in dlp that have been replaced with Ala. Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Nakato et al., 2024)

point_mutation

3L:14,670,211..14,670,211 [+]

Nucleotide change:

T14670211G

Amino acid change:

S631A | dlp-PA; S630A | dlp-PC

Reported amino acid change:

S631A

Comment:

Third of five glycosaminoglycan (GAG)-attachment serine residues in dlp that have been replaced with Ala. Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Nakato et al., 2024)

point_mutation

3L:14,670,247..14,670,247 [+]

Nucleotide change:

T14670247G

Amino acid change:

S643A | dlp-PA; S642A | dlp-PC

Reported amino acid change:

S643A

Comment:

Fourth of five glycosaminoglycan (GAG)-attachment serine residues in dlp that have been replaced with Ala. Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Nakato et al., 2024)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Nakato et al., 2024)

viable

(Nakato et al., 2024)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

dlp^ΔGAG is an enhancer of increased mortality during development phenotype of dally^ΔGAG

(Nakato et al., 2024)

dlp^ΔGAG is an enhancer of increased mortality during development phenotype of dally^gem

(Nakato et al., 2024)

Suppressor of

Statement

Reference

dlp^ΔGAG is a suppressor of visible | adult stage phenotype of dally^ΔGAG

(Nakato et al., 2024)

Other

Statement

Reference

dally^gem, dlp^ΔGAG has lethal phenotype

(Nakato et al., 2024)

dally^ΔGAG, dlp^ΔGAG has partially lethal phenotype

(Nakato et al., 2024)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

dlp^ΔGAG/dally^ΔGAG is a non-enhancer of wing disc dorsal compartment phenotype of Scer\GAL4^ap.PU, Sulf1^{Golgi.UAS.Tag:Golgi(hGALNT3)}

(Nakato et al., 2024)

Suppressor of

Statement

Reference

dlp^ΔGAG is a suppressor of wing vein | ectopic phenotype of dally^ΔGAG

(Nakato et al., 2024)

NOT Suppressor of

Statement

Reference

dlp^ΔGAG/dally^ΔGAG is a non-suppressor of wing disc dorsal compartment phenotype of Scer\GAL4^ap.PU, Sulf1^{Golgi.UAS.Tag:Golgi(hGALNT3)}

(Nakato et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dlp^ΔGAG

(Nakato et al., 2024)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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