FlyBase Allele Report: Dmel\dsh[JF01253]

General Information

Symbol

Dmel\dsh^JF01253

Species

D. melanogaster

Name

FlyBase ID

FBal0245545

Feature type

allele

Associated gene

Dmel\dsh

Associated Insertion(s)

Carried in Construct

P{TRiP.JF01253}

Also Known As

UAS-dsh RNAi, dshRNAi, dsh RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

(Ni et al., 2008)

Progenitor genotype

Carried in construct

P{TRiP.JF01253}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Ni et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF01253

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage | conditional, with Scer\GAL4^elav-C155

(Dominicci-Cotto et al., 2024)

decreased cell number | oogenesis, with Scer\GAL4^C587

(Tu et al., 2020)

increased cell number | adult stage, with Scer\GAL4^NP0001

(Wang et al., 2016)

increased cell number | oogenesis, with Scer\GAL4^C587

(Tu et al., 2020)

Phenotype Manifest In

adult midgut, with Scer\GAL4^NP0001

(Wang et al., 2016)

egg chamber, with Scer\GAL4^c306

(Dai et al., 2017)

embryonic/larval neuromuscular junction | larval stage | conditional, with Scer\GAL4^elav-C155

(Dominicci-Cotto et al., 2024)

enteroblast | adult stage, with Scer\GAL4^NP0001

(Wang et al., 2016)

escort cell | decreased number, with Scer\GAL4^C587

(Tu et al., 2020)

female germline cell | increased number, with Scer\GAL4^C587

(Tu et al., 2020)

germarium, with Scer\GAL4^C587

(Tu et al., 2020)

ovariole, with Scer\GAL4^c306

(Dai et al., 2017)

Detailed Description

Statement

Reference

The expression of dsh^JF01253 under the control of Scer\GAL4^c306 leads to a severe increase in the proportion of ovarioles showing fused egg chambers, as compared to controls.

(Dai et al., 2017)

Expression of dsh^JF01253 under the control of Scer\GAL4^{Myo31DF-NP0001}, but not of Scer\GAL4^esg-NP5130, in combination with gal80[ts] to restrict expression to adulthood, leads to a significant increase in the number of progenitor cells in the midgut, as compared to controls.

(Wang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^C587, dsh^JF01253 has decreased cell number | oogenesis phenotype, suppressible | partially by dlp^HMS00875/Scer\GAL4^C587

(Tu et al., 2020)

Scer\GAL4^C587, dsh^JF01253 has increased cell number | oogenesis phenotype, suppressible | partially by dlp^HMS00875/Scer\GAL4^C587

(Tu et al., 2020)

Scer\GAL4^C587, dsh^JF01253 has increased cell number | oogenesis phenotype, suppressible | partially by dlp^MI04217

(Tu et al., 2020)

Scer\GAL4^C587, dsh^JF01253 has increased cell number | oogenesis phenotype, suppressible | partially by dlp^MI09937

(Tu et al., 2020)

Scer\GAL4^C587, dsh^JF01253 has increased cell number | oogenesis phenotype, suppressible | partially by dlp^MI10046

(Tu et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^c306, dsh^JF01253 has egg chamber phenotype, enhanceable by Scer\GAL4^c306/smo^HMJ30064

(Dai et al., 2017)

Scer\GAL4^c306, dsh^JF01253 has ovariole phenotype, enhanceable by Scer\GAL4^c306/smo^HMJ30064

(Dai et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^C587, dsh^JF01253 has germarium phenotype, suppressible | partially by dlp^HMS00875/Scer\GAL4^C587

(Tu et al., 2020)

Scer\GAL4^C587, dsh^JF01253 has female germline cell | increased number phenotype, suppressible | partially by dlp^HMS00875/Scer\GAL4^C587

(Tu et al., 2020)

Scer\GAL4^C587, dsh^JF01253 has female germline cell | increased number phenotype, suppressible | partially by dlp^MI04217

(Tu et al., 2020)

Scer\GAL4^C587, dsh^JF01253 has female germline cell | increased number phenotype, suppressible | partially by dlp^MI10046

(Tu et al., 2020)

Scer\GAL4^C587, dsh^JF01253 has female germline cell | increased number phenotype, suppressible | partially by dlp^MI09937

(Tu et al., 2020)

Scer\GAL4^C587, dsh^JF01253 has escort cell | decreased number phenotype, suppressible | partially by dlp^HMS00875/Scer\GAL4^C587

(Tu et al., 2020)

Enhancer of

Statement

Reference

dsh^JF01253/Scer\GAL4^c306 is an enhancer of egg chamber phenotype of Scer\GAL4^c306, smo^HMJ30064

(Dai et al., 2017)

dsh^JF01253/Scer\GAL4^c306 is an enhancer of ovariole phenotype of Scer\GAL4^c306, smo^HMJ30064

(Dai et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of smo^HMJ30064 and dsh^JF01253 under the control of Scer\GAL4^c306 leads to a more severe egg-fusion phenotype than the corresponding single expression conditions.

(Dai et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

31306

y¹ v¹; P{TRiP.JF01253}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dsh^JF01253

dsh^KD

(Dai et al., 2017)

dsh^dsRNA

(Luo et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

Open Close