FlyBase Allele Report: Dmel\Top2[JF01300]

General Information

Symbol

Dmel\Top2^JF01300

Species

D. melanogaster

Name

FlyBase ID

FBal0245591

Feature type

allele

Associated gene

Dmel\Top2

Associated Insertion(s)

Carried in Construct

P{TRiP.JF01300}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

(Ni et al., 2008)

Progenitor genotype

Carried in construct

P{TRiP.JF01300}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Ni et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF01300

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Jiao et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased size | adult stage, with Scer\GAL4^en.PU

(Yang et al., 2021)

decreased size | pharate adult stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

increased cell death | third instar larval stage, with Scer\GAL4^en.PU

(Yang et al., 2021)

lethal - all die before end of P-stage | male limited, with Scer\GAL4^ey.PU

(Yang et al., 2021)

majority die during P-stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

partially lethal - majority die, with Scer\GAL4^ey.PU

(Yang et al., 2021)

viable, with Scer\GAL4^en.PU

(Yang et al., 2021)

viable | female limited, with Scer\GAL4^ey.PU

(Yang et al., 2021)

Phenotype Manifest In

eye | absent | adult stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

eye | adult stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

nucleus | third instar larval stage, with Scer\GAL4^en.PU

(Yang et al., 2021)

posterior crossvein, with Scer\GAL4^en.PU

(Yang et al., 2021)

wing, with Scer\GAL4^en.PU

(Yang et al., 2021)

wing, with Scer\GAL4^nub.PU

(Yang et al., 2021)

wing cell, with Scer\GAL4^en.PU

(Yang et al., 2021)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en.PU

(Yang et al., 2021)

wing vein, with Scer\GAL4^en.PU

(Yang et al., 2021)

wing vein L4, with Scer\GAL4^en.PU

(Yang et al., 2021)

Detailed Description

Statement

Reference

The expression of Top2^JF01300 under the control of Scer\GAL4^en.PU induces wing defects, including a small wing, and a reduction or loss of the crossvein L4 and/or the posterior crossvein; the third instar larval wing discs show increased cell death (Dcp1 staining) and basal mislocalization of many cell nuclei in the posterior domain.

All surviving adults expressing Top2^JF01300 under the control of Scer\GAL4^ey.PU exhibit small to absent eyes.

Adults expressing Top2^JF01300 under the control of Scer\GAL4^nub.PU exhibit a small wing, which can also be severely wrinkled.

(Yang et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Top2^JF01300/Scer\GAL4^GMR.long is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, Zzzz\GGGGCC^30.UAS.EGFP