FlyBase Allele Report: Dmel\solo[Z2-0198]

General Information

Symbol

Dmel\solo^Z2-0198

Species

D. melanogaster

Name

FlyBase ID

FBal0246479

Feature type

allele

Associated gene

Dmel\solo

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Yan et al., 2010)

Mutagen

ethyl methanesulfonate

(Yan et al., 2010)

Progenitor genotype

Cytology

Description

Amino acid replacement: W387term.

(Yan et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:15,069,781..15,069,781 [+]

Nucleotide change:

G15069781A

Amino acid change:

W387term | solo-PA; W387term | solo-PB

Reported amino acid change:

W387term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Yan et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | female (with Df(2L)A267)

(Yan and McKee, 2013)

abnormal meiotic cell cycle | female (with solo⁷)

(Yan and McKee, 2013)

abnormal meiotic cell cycle | male (with Df(2L)A267)

(Yan et al., 2010)

abnormal meiotic cell cycle | male (with solo^Z2-3534)

(Yan et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

solo^Z2-0198/Df(2L)A267 females show 55.1% X chromosome nondisjunction. Crossing over on the X chromosome is substantially and uniformly reduced in three euchromatic intervals and more moderately reduced in the interval encompassing the X centromere. Sister chromatid exchange on the X chromosome is increased compared to controls.

solo⁷/solo^Z2-0198 females show chromosome 2 nondisjunction.

(Yan and McKee, 2013)

solo^Z2-0198/Df(2L)A267 males show a high frequency (56.4%) of sex chromosome nondisjunction in meiosis. Mutant spermatocytes show a more complex meiosis I chromatid segregation pattern than in wild type. Meiosis II is visibly abnormal in the mutant spermatocytes; more than 90% of spermatocytes in meiosis II have DNA masses that are both smaller and more numerous than in wild-type males (indicative of precocious sister chromatid separation), chromosome segregation at anaphase II is disorganised (approximately 38% of anaphase II nuclei have lagging chromosomes) and meiosis II poles show clearly unequal amounts of DNA in 88% of nuclei (indicating high levels of meiosis II nondisjunction).

solo^Z2-3534/solo^Z2-0198 males show a high frequency (54.1%) of sex chromosome nondisjunction in meiosis.

(Yan et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Crossover frequency in the X euchromatin (pn-f interval) is reduced 65-fold in solo^Z2-3534 ord^Z5736/solo^Z2-0198 ord⁵ females compared to doubly heterozygous sibling controls. This reduction is intermediate in value between those of ord or solo single mutants.

(Yan and McKee, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

(Yan et al., 2010)

(Yan et al., 2010)

(Yan et al., 2010)

(Yan et al., 2010)

(Yan et al., 2010)

(Yan et al., 2010)

Fails to complement

vas^6356-001

(Yan et al., 2010)

vas^RJ36

(Yan et al., 2010)

Rescued by

solo^Z2-0198 is rescued by Scer\GAL4^{VP16.nanos.UTR}/solo^N.UASp.Venus

(Yan and McKee, 2013)

solo^Z2-0198 is rescued by Scer\GAL4^{VP16.nanos.UTR}/solo^C.UASp.Venus

(Yan and McKee, 2013)

solo^Z2-0198 is rescued by solo^{UASp.Tag:FLAG,Tag:HA}/Scer\GAL4^{VP16.nanos.UTR}

(Yan and McKee, 2013)

Partially rescued by

solo^Z2-0198 is partially rescued by solo^Venus

(Yan and McKee, 2013)

Comments

The chromosome nondisjunction phenotype seen in solo^Z2-0198/Df(2L)A267 females is fully rescued by expression of either solo^{N.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus}, solo^{C.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} or solo^{Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

The chromosome nondisjunction phenotype seen in solo^Z2-0198/Df(2L)A267 females is partially rescued by solo^{T:Avic\GFP-YFP.Venus}.

Expression of vas^T:Avic\GFP in solo^Z2-0198/Df(2L)A267 females substantially improves the number of germ cell cysts and nearly doubles fertility (number of progeny/female), indicating that these phenotypes are not due to loss of solo function, but are instead due to an as yet unexplained inhibitory effect of the solo^Z2-0198 allele on the expression of vas.

(Yan and McKee, 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

606957

solo^Z2-0198 cn¹ bw¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

solo^Z2-0198

(Blattner et al., 2016, Krishnan et al., 2014, Yan and McKee, 2013, Yan et al., 2010)

vas^solo-Z2-0198

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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