FlyBase Allele Report: Dmel\mim[null]

General Information

Symbol

Dmel\mim^null

Species

D. melanogaster

Name

FlyBase ID

FBal0248904

Feature type

allele

Associated gene

Dmel\mim

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Quinones et al., 2010)

Mutagen

recombination

(Quinones et al., 2010)

FLPase

(Quinones et al., 2010)

I-SceI

(Quinones et al., 2010)

ends-out gene targeting

(Quinones et al., 2010)

Progenitor genotype

Cytology

Description

Deletion of exons 3-10.

(Quinones et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:6,933,655..6,940,244 [-]

Comment:

Deletion made by modified "ends-out" homologous recombination that is reported to remove exons 3-10 (endpoints derived from primer sequences).

(Quinones et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage

(Quinones et al., 2010)

female semi-sterile

(Quinones et al., 2010)

Phenotype Manifest In

border follicle cell

(Quinones et al., 2010)

germline cell

(Quinones et al., 2010)

Detailed Description

Statement

Reference

Mutant adults appear normal but display defects in locomotor activity, and mutant females show partial sterility.

Approximately 10% of the primordial germ cells arrest outside the embryo after germ band extension in mutant animals. Of those that can enter the embryo, all are capable of transmigration, but only 20% appropriately migrate towards the gonadal mesoderm. The remaining primordial germ cells are scattered throughout the posterior embryos.

Border follicle cell clusters are normal in number and size in mutant egg chambers, but are significantly delayed in the initial phase of migration compared to control clusters, such that at stage 10, nearly half of the mutant border cells are still delayed, whereas more than 90% of wild-type clusters have traversed the egg chamber to the oocyte. Analysis of migration in the mutant egg chambers shows that the directionality index of the mutant border cell clusters is reduced compared to wild-type clusters and that the speed of migration independent of direction is also reduced in the mutant border cell clusters compared to wild type.

Mutant border cells show an increased rate of uptake of the lipophilic dye FM4-64 compared to wild-type controls.

(Quinones et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

mim^null has border follicle cell phenotype, suppressible by Cortactin^M7

(Quinones et al., 2010)

mim^null has border follicle cell phenotype, suppressible by cindr^RNAi.UAS/Scer\GAL4³⁰⁶

(Quinones et al., 2010)

mim^null has border follicle cell phenotype, suppressible by Scer\GAL4³⁰⁶/cindr^{RNAi.PC.PD.UAS}

(Quinones et al., 2010)

Suppressor of

Statement

Reference

mim^null is a suppressor of border follicle cell phenotype of Scer\GAL4³⁰⁶, cindr^{RNAi.PC.PD.UAS}

(Quinones et al., 2010)

mim^null is a suppressor of border follicle cell phenotype of Scer\GAL4³⁰⁶, cindr^RNAi.UAS

(Quinones et al., 2010)

mim^null is a suppressor of border follicle cell phenotype of Cortactin^M7

(Quinones et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

mim^null Cortactin^M7 double mutants have a wild-type border cell migration phenotype in the egg chamber.

Expression of cindr^{dsRNA.Scer\UAS} under the control of Scer\GAL4³⁰⁶ in a mim^null background restores the ability of border cells in the egg chamber to migrate.

Expression of cindr^{dsRNA.PC.PD.Scer\UAS} under the control of Scer\GAL4³⁰⁶ in a mim^null background restores the ability of border cells in the egg chamber to migrate.

(Quinones et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mim^null is rescued by mim^{UAS.Tag:MYC,Tag:polyHis}/Scer\GAL4³⁰⁶

(Quinones et al., 2010)

Comments

Expression of mim^{Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} under the control of Scer\GAL4³⁰⁶ rescues the border cell migration defects seen in mim^null egg chambers.

(Quinones et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

MIM^null

(Biton et al., 2023)

dmim-null

(Quinones et al., 2010)

mim^null

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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