FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rab11S25N.UAS
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General Information
Symbol
Dmel\Rab11S25N.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0248957
Feature type
allele
Associated gene
Dmel\Rab11
Associated Insertion(s)
Carried in Construct
P{UAS-Rab11.S25N}
Key Links
Transgenic product class
missense_variant
(Assaker et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Assaker et al., 2010)
Mutagen
in vitro construct
(Assaker et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-Rab11.S25N}
Cytology
Description

UAS regulatory sequences drive expression of a Ser[25] to Asn mutant form of Rab11.

(Assaker et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Rab11
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Rab11S25N.Scer\UAS in border cells under the control of Scer\GAL4c306 leads to a strong migration block.

      Expression of Rab11S25N.Scer\UAS under the control of Scer\GAL4slbo.2.6 slightly reduces the volume distribution of sec15-positive structures at the leading edge of border cells.

      (Laflamme et al., 2012)

      Embryos expressing Rab11S25N.Scer\UAS under the control of Scer\GAL4da.G32 show a loss of epidermal integrity reflected in the crumbled cuticle laid by these embryos.

      Expression of Rab11S25N.Scer\UAS under the control of Scer\GAL4c381 does not impair epidermal cell elongation during dorsal closure stages, although zippering is defective. Dorsal closure is completed in these embryos.

      Expression of Rab11S25N.Scer\UAS under the control of Scer\GAL4en-e16E impairs epidermal elongation during dorsal closure stages and results in a delay in the advancement of the leading edge towards the dorsal midline, which eventually stalls. This results in embryos with deep ectopic segmental grooves and a large dorsal hole in the cuticle.

      (Mateus et al., 2011)

      Expression of Rab11S25N.Scer\UAS in the eye disc under the control of Scer\GAL4GMR.PF causes lamina cartridge neuron differentiation defects in third instar larvae. R8 photoreceptor differentiation is unaffected. The resulting eye are small and rough.

      Expression of Rab11S25N.Scer\UAS in R8 photoreceptors under the control of Scer\GAL4sca-537.4 results in missing photoreceptors and misrotated ommatidia but lamina cartridge neuron differentiation is unaffected.

      (Yogev et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Rab11S25N.UAS has eye phenotype, suppressible | partially by Scer\GAL4GMR.PF/rhoUAS.EGFP

      (Yogev et al., 2010)

      Rab11S25N.UAS has eye phenotype, suppressible | partially by ruUAS.EGFP/Scer\GAL4GMR.PF

      (Yogev et al., 2010)
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      shgUbi-p63E.T:Avic\GFP-rs does not significantly change the impaired epidermal cell elongation phenotype seen during dorsal closure stages in embryos expressing Rab11S25N.Scer\UAS under the control of Scer\GAL4en-e16E.

      Co-expression of shgScer\UAS.T:Avic\GFP-rs does not significantly change the impaired epidermal cell elongation phenotype seen during dorsal closure stages in embryos expressing Rab11S25N.Scer\UAS under the control of Scer\GAL4en-e16E.

      Epidermal cells expressing Rab11S25N.Scer\UAS under the control of Scer\GAL4en-e16E in a shgR64a background do not elongate during dorsal closure stages and detach from the amnioserosa. The embryos have very large dorsal holes and have lateral holes in the cuticle.

      (Mateus et al., 2011)

      Expression of rhoScer\UAS.T:Avic\GFP-EGFP partially rescues the small, rough eyes seen when Rab11S25N.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

      Expression of ruScer\UAS.T:Avic\GFP-EGFP partially rescues the small, rough eyes seen when Rab11S25N.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

      Expression of Rab11S25N.Scer\UAS suppresses the enlarged lamina seen in third instar larval eye discs when Ras85DV12.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

      (Yogev et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rab11S25N.Scer\UAS
      Rab11S25N.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (5)