FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4GMR21D02
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General Information
Symbol
Scer\GAL4GMR21D02
Species
S. cerevisiae
Name
FlyBase ID
FBal0250553
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GMR21D02-GAL4}
Also Known As
R21D02, R21D02-GAL4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2011.2.9)
Progenitor genotype
Carried in construct
P{GMR21D02-GAL4}
Cytology
Description

A 3965 base pair fragment from the flanking non-coding or intronic region of nAcRalpha-30D is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR21D02
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval mushroom body output neuron b2
      (Saumweber et al., 2018, Eichler et al., 2017)
      larval mushroom body output neuron b1
      (Saumweber et al., 2018, Eichler et al., 2017)
      larval mushroom body dopaminergic neuron d1
      (Saumweber et al., 2018)
      larval mushroom body dopaminergic neuron f1
      (Saumweber et al., 2018)
      larval mushroom body dopaminergic neuron g1
      (Saumweber et al., 2018)
      larval mushroom body dopaminergic neuron i1
      (Saumweber et al., 2018)
      larval mushroom body output neuron i1
      (Saumweber et al., 2018)
      larval mushroom body dopaminergic neuron k1
      (Saumweber et al., 2018)
      larval mushroom body output neuron k1
      (Saumweber et al., 2018)
      larval mushroom body anterior paired lateral cell
      (Saumweber et al., 2018)
      adult stage
      adult lateral horn | faint | restricted
      (Jenett and Svirskas, 2012.9.24)
      mushroom body | faint | restricted
      (Jenett and Svirskas, 2012.9.24)
      mushroom body output neuron 2
      (Owald et al., 2015)
      mushroom body output neuron 3
      (Owald et al., 2015)
      mushroom body output neuron 1
      (Owald et al., 2015)
      adult brain | restricted
      (Shuai et al., 2015)
      mushroom body extrinsic neuron
      (Shuai et al., 2015)
      mushroom body gamma lobe slice 5
      (Shuai et al., 2015)
      mushroom body beta lobe slice 2
      (Shuai et al., 2015)
      mushroom body beta' lobe slice 2
      (Shuai et al., 2015)
      Additional Information
      Statement
      Reference

      ScerGAL4GMR21D02 drives expression in VGlut1 positive mushroom body output neuron 2, mushroom body medial lobe arborizing neuron 4 and mushroom body medial lobe arborizing neuron 6.

      (Owald et al., 2015)

      ScerGAL4GMR21D02 drives expression in mushroom body extrinsic neurons that innervate mushroom body gamma lobe slice 3, mushroom body beta lobe slice 2 and mushroom body beta' lobe slice 2.

      (Shuai et al., 2015)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Blocking synaptic transmission (using shi1.Scer\UAS at 32[o]C) from Scer\GAL4GMR21D02, Scer\GAL4VT001211, or Scer\GAL4GMR66C08 neurons significantly impairs memory performance compared to controls. With sucrose-reinforced appetitive conditioning or electric shock-reinforced aversive conditioning, immediate memory at 3 minutes post-conditioning is impaired (both training and testing at a restrictive 32[o]C temperature). Appetitive conditioning memory at 24 hours is also impaired (training at a permissive temperature of 23[o]C, testing at the restrictive temperature).

      Blocking synaptic transmission (using shi1.Scer\UAS at 32[o]C) from Scer\GAL4GMR21D02 or Scer\GAL4VT001211 neurons significantly abolishes or reverses (depending on the odorant and concentration used) naive odor avoidance behavior, compared to controls.

      Optogenetic activation (using Crei\ChR1[ReaChR.Scer\UAS. T:Crei \ChR2, T:Avic \GFP-mCitrine] with red light activation) of Scer\GAL4GMR21D02 neurons results in significant avoidance of the red-light illuminated arm in a T-maze.

      (Owald et al., 2015)

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4GMR21D02 neurons, in combination with a Gal80[ts] transgene to restrict expression to 1 day prior to training and testing, does not affect 3 hour aversive olfactory memory, compared to controls.

      (Shuai et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4GMR21D02
      Name Synonyms
      Secondary FlyBase IDs
        References (9)