FlyBase Allele Report: Scer\GAL4[GMR27G01]

General Information

Symbol

Scer\GAL4^GMR27G01

Species

S. cerevisiae

Name

FlyBase ID

FBal0251146

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GMR27G01-GAL4}

Also Known As

R27G01, R27G01-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2011.2.9)

Progenitor genotype

Carried in construct

P{GMR27G01-GAL4}

Cytology

Description

A 2091 base pair fragment from the flanking non-coding or intronic region of Lmpt is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR27G01

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body output neuron k1

(Eichler et al., 2017)

larval mushroom body dopaminergic neuron g1

(Eichler et al., 2017)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Sun et al., 2018)

abnormal memory | adult stage, with VGlut1^KK107498

(Shyu et al., 2017)

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Bouzaiane et al., 2015)

abnormal memory | adult stage | heat sensitive, with shi^{ts1.IVS.p10.20xUAS}

(Shyu et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using shi^1.UAS at 32[o]C) of Scer\GAL4^GMR27G01 cells in adults does not affect startle-induced climbing behavior, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^GMR27G01 cells in adults significantly reduces startle-induced climbing behavior, compared to controls.

(Sun et al., 2018)

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 32[o]C) of Scer\GAL4^GMR27G01 cells in adults during retrieval alone, but not acquisition and consolidation, significantly reduces 24-hour associative olfactory water-reward memory, compared to controls, with no effect on 3-hour memory; inhibition has no effect on odor acuity or water preference, compared to controls.

(Shyu et al., 2017)

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^GMR27G01 cells in adults significantly reduces short term (5 min) aversive olfactory memory, but not its anaesthesia-resistant component, compared to controls; inhibition during testing significantly reduces medium term (3 h) aversive olfactory memory, including its anaesthesia-resistant component, and long term (24 h after massed, but not spaced, training) aversive olfactory memory, compared to controls; inhibition during and after testing (excluding the memory test) does not affect medium term (3 h) aversive olfactory memory, compared to controls; inhibition does not affect olfactory acuity, compared to controls.

(Bouzaiane et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions