FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4GMR48B04
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General Information
Symbol
Scer\GAL4GMR48B04
Species
S. cerevisiae
Name
FlyBase ID
FBal0252911
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GMR48B04-GAL4}
Also Known As
R48B04-Gal4, R48B04
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2011.2.9)
Progenitor genotype
Carried in construct
P{GMR48B04-GAL4}
Cytology
Description

A 2724 base pair fragment from the flanking non-coding or intronic region of Oamb is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR48B04
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      prow | faint | restricted
      (Jenett and Svirskas, 2012.9.24)
      adult brain | restricted
      (Shyu et al., 2017, Huetteroth et al., 2015, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)
      mushroom body extrinsic neuron
      (Shuai et al., 2015)
      mushroom body gamma lobe slice 3
      (Shuai et al., 2015, Yamagata et al., 2015)
      mushroom body gamma lobe slice 4
      (Shyu et al., 2017, Huetteroth et al., 2015, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)
      mushroom body gamma lobe slice 5
      (Shyu et al., 2017, Huetteroth et al., 2015, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)
      mushroom body beta' lobe slice 2
      (Shyu et al., 2017, Huetteroth et al., 2015, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)
      adult dopaminergic PAM neuron | subset
      (Shyu et al., 2017, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)
      mushroom body gamma lobe slice 1
      (Shyu et al., 2017, Huetteroth et al., 2015)
      mushroom body gamma lobe slice 2
      (Shyu et al., 2017, Huetteroth et al., 2015, Yamagata et al., 2015)
      dopaminergic PAM neuron 1
      (Shyu et al., 2017, Huetteroth et al., 2015, Lin et al., 2014)
      dopaminergic PAM neuron 2
      (Shyu et al., 2017, Huetteroth et al., 2015, Lin et al., 2014)
      dopaminergic PAM neuron 6
      (Shyu et al., 2017, Huetteroth et al., 2015, Lin et al., 2014)
      dopaminergic PAM neuron 5
      (Shyu et al., 2017, Huetteroth et al., 2015, Lin et al., 2014)
      TH negative neuron associated with PAM cluster
      (Huetteroth et al., 2015)
      dopaminergic neuron | subset
      (Huetteroth et al., 2015)
      dopaminergic PAM neuron 8
      (Shyu et al., 2017, Lin et al., 2014)
      mushroom body beta lobe slice 1
      (Yamagata et al., 2015)
      Additional Information
      Statement
      Reference

      ScerGAL4GMR48B04 drives expression in a subset of dopaminergic PAM neurons, including those that project to mushroom body gamma lobe slices 1, 2, 4, 5 and mushroom body beta' lobe slice 2.

      (Shyu et al., 2017)

      ScerGAL4GMR48B04 drives expression in mushroom body extrinsic neurons, including approximately 60 dopaminergic PAM neurons, that innervate mushroom body gamma lobe slices 3, 4 and 5, and mushroom body beta' lobe slice 2.

      (Shuai et al., 2015)

      ScerGAL4GMR48B04 labels a subset of dopaminergic neurons including ~55 dopaminergic PAM neurons and ~12 TH negative neuron associated with PAM clusters. Collectively, these neurons innervate mushroom body gamma lobe slices 1, 2, 4 & 5 and mushroom body beta' lobe slice 2.

      (Huetteroth et al., 2015)

      ScerGAL4GMR48B04 drives expression in a subset of PAM neurons that innervate mushroom body beta' lobe slice 2, beta lobe slice 1 and gamma lobe slices 1 to 5.

      (Yamagata et al., 2015)
       
      Marker for
      Reflects expression of
      Oamb
      (Huetteroth et al., 2015, Lin et al., 2014)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using shits1.IVS.p10.20xUAS at 32[o]C) of Scer\GAL4GMR48B04 cells in adults significantly reduces 3-min associative olfactory water-reward memory, compared to controls; inhibition during acquisition alone also significantly reduces 3-hour, but not 24-hour memory.

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 31[o]C) of Scer\GAL4GMR48B04 cells in thirsty adults, in combination with a paired odor, results in a significant increase in 3-min and 3-hour, but not 24-hour, associative attractive olfactory memory, compared to controls; increased 3-min and 3-hour memory is also observed in water-sated adults.

      (Shyu et al., 2017)

      Blocking the output (using shi1.UAS at 33[o]C) of Scer\GAL4GMR48B04 neurons shortly before and during training significantly impairs short-term, but not long-term, olfactory memory in starved flies trained with sucrose, compared to controls.

      Acute thermogenetic activation (using TrpA1UAS.(B).cKa at 33[o]C) of Scer\GAL4GMR48B04 neurons results in a significant short-term preference for a paired odor (artificial memory) in starved or fed flies, compared to controls; this preference is no longer seen after 24 hours.

      (Huetteroth et al., 2015)

      Inhibition (using Hsap\KCNJ2UAS.EGFP or Ctet\TeTxLCTNT.UAS) of Scer\GAL4GMR48B04 neurons, in combination with a Gal80[ts] transgene to restrict expression to 1 day prior to training and testing, does not affect 3 hour aversive olfactory memory, compared to controls.

      (Shuai et al., 2015)

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of Scer\GAL4GMR48B04 cells in adults, in combination with a paired odor, results in a significant increase in short-term, but not long-term, associative attractive olfactory memory, compared to controls; memory induction does not occur when activation is restricted to a smaller subset of cells using Scer\GAL80R58E02.

      Inhibition (using shits1.IVS.p10.20xUAS at 33[o]C) of Scer\GAL4GMR48B04 cells in adults during training significantly reduces short-term, but not long-term, associative appetitive (sugar) olfactory memory, compared to controls; memory reduction does not occur when activation is restricted to a smaller subset of cells using Scer\GAL80R58E02.

      (Yamagata et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4GMR48B04
      Name Synonyms
      Secondary FlyBase IDs
        References (15)