FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4GMR76B09
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General Information
Symbol
Scer\GAL4GMR76B09
Species
S. cerevisiae
Name
FlyBase ID
FBal0254818
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{GMR76B09-GAL4}
Also Known As
R76B09-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2011.2.9)
Progenitor genotype
Carried in construct
P{GMR76B09-GAL4}
Cytology
Description

A 961 base pair fragment from the flanking non-coding or intronic region of GABA-B-R3 is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR76B09
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult brain | restricted
      (Shuai et al., 2015)
      mushroom body extrinsic neuron
      (Shuai et al., 2015)
      mushroom body gamma lobe slice 1
      (Shuai et al., 2015)
      mushroom body gamma lobe slice 2
      (Shuai et al., 2015)
      mushroom body gamma lobe slice 4
      (Shuai et al., 2015)
      mushroom body beta lobe slice 1
      (Shuai et al., 2015)
      mushroom body alpha lobe slice 1
      (Shuai et al., 2015)
      mushroom body alpha lobe slice 2
      (Shuai et al., 2015)
      mushroom body alpha lobe slice 3
      (Shuai et al., 2015)
      mushroom body output neuron 5
      (Shuai et al., 2015)
      mushroom body output neuron 6 | faint
      (Shuai et al., 2015)
      Additional Information
      Statement
      Reference

      ScerGAL4GMR76B09 drives expression in mushroom body extrinsic neurons, including mushroom body output neuron 5 and mushroom body medial-vertical lobe arborizing neuron 2, that innervate mushroom body gamma lobe slices 1, 2 and 4, mushroom body beta lobe slice 1, and mushroom body alpha lobe slices 1, 2 and 3. Expression overlaps with that driven by EcollexAGMR24E12 in mushroom body output neuron 5.

      (Shuai et al., 2015)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4GMR76B09 neurons, in combination with a Gal80[ts] transgene to restrict expression to 1 day prior to training and testing, significantly improves long term (1 hour, 3 hour and 24 hour), but not immediate short term, aversive olfactory memory, compared to controls; 3 hour memory following a 2 min cold shock is similar to that of controls; there is no effect on reversal learning, shock reactivity or olfactory acuity, compared to controls.

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of the combined set of Scer\GAL4GMR76B09 neurons and Scer\GAL4NP2397 neurons, in combination with a Gal80[ts] transgene to restrict expression to 1 day prior to training and testing, significantly improves long term aversive olfactory memory across multiple time points, to a greater extent than either group alone (additive), compared to controls; there is no effect on reversal learning, compared to controls.

      Thermogenetic activation (using TrpA1UAS.cUa at 32[o]C) of Scer\GAL4GMR76B09 neurons for the 20 min immediately after training, but not the 20 min immediately before training, significantly reduces 3 hour aversive olfactory memory, compared to controls.

      (Shuai et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4GMR76B09
      Name Synonyms
      Secondary FlyBase IDs
        References (4)