A 2587 base pair fragment from the flanking non-coding or intronic region of Lsp1gamma is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.
abnormal phototaxis | larval stage, with Ctet\tetXTNT-LC.UAS
abnormal phototaxis | larval stage, with Ctet\tetXTNT-LC.UAS, Scer\GAL80ChAT.PU
abnormal phototaxis | larval stage, with RdlHMC03643
hyperactive | larval stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus
larval head | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus
Inhibition (using Ctet\TeTxLCTNT.UAS) of Scer\GAL4GMR82B10 cells significantly reduces larval avoidance of white light, compared to controls; this phenotype is still seen when activation is restricted to a smaller set of cells using Scer\GAL80ChAT.PU.
Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 620 nm (red) light) of Scer\GAL4GMR82B10 cells significantly increases larval head casts, compared to controls.