FlyBase Allele Report: Dmel\mir-263b[Δ]

General Information

Symbol

Dmel\mir-263b^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0257044

Feature type

allele

Associated gene

Dmel\mir-263b

Associated Insertion(s)

TI{TI}mir-263b^Δ

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)

Mutagen

recombination

(Hilgers et al., 2010)

ends-out gene targeting

(Hilgers et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{TI}mir-263b^Δ

Cytology

Description

Knock-out of the mir-263b gene. The expression of the CG32150 host gene appears unaffected (assayed by quantitative PCR).

(Chen et al., 2014)

Knock-out of mir-263b. A single loxP site is present in place of the deleted sequence.

(Cohen, 2014.12.11)

Deletion that removes mir-263b. A w[+] marker that that was originally inserted as part of the mutagenic event has been excised.

(Hilgers et al., 2010)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon collateral | adult stage

(Nian et al., 2019)

interommatidial bristle (with Df(3L)X-21.2)

(Hilgers et al., 2010)

s-LNv Pdf neuron

(Nian et al., 2019)

Detailed Description

Statement

Reference

mir-263b^Δ/mir-263b^Δ and mir-263b^Δ/mir-263b^Δ-G4 adults show decreased circadian rhythmicity and circadian power under constant dark, as compared to wild-type and mir-263b^Δ-G4 heterozygous controls. mir-263b^Δ/mir-263b^Δ and mir-263b^Δ/mir-263b^Δ-G4 adults also exhibit a lack of morning anticipation activity, as compared to wild-type controls.

mir-263b^Δ/mir-263b^Δ adults show disrupted circadian plasticity of sLNv dorsal projections: while controls exhibit more axon terminal branches at ZT2 than at ZT14 under light-dark cycles or dark conditions, mutants present similar number of axon terminal branches at (presumptive) ZT2 and ZT14, which are similar to ZT14 in controls.

(Nian et al., 2019)

The posterior midgut of 14 days old mir-263b^Δ homozygotes displays a similar number of mitotic cells, as compared to controls.

(Kim et al., 2017)

mir-263b^Δ/Df(3L)X-21.2 flies show a modest reduction in the number of interommatidial bristles.

(Hilgers et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Df(3L)X-21.2/mir-263b^Δ is an enhancer of visible phenotype of mir-bft²⁴/mir-bft^Δ263a

(Hilgers et al., 2010)

NOT Enhancer of

Statement

Reference

mir-263b^Δ/mir-263b^Δ is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^Pdf.PU, TrpA1^UAS.(B).cKa

(Nian et al., 2019)

NOT Suppressor of

Statement

Reference

mir-263b^Δ/mir-263b^Δ is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^Pdf.PU, TrpA1^UAS.(B).cKa

(Nian et al., 2019)

Phenotype Manifest In

Enhancer of

Statement

Reference

Df(3L)X-21.2/mir-263b^Δ is an enhancer of interommatidial bristle phenotype of mir-bft²⁴/mir-bft^Δ263a

(Hilgers et al., 2010)

NOT Enhancer of

Statement

Reference

mir-263b^Δ/mir-263b^Δ is a non-enhancer of s-LNv Pdf neuron phenotype of Scer\GAL4^Pdf.PU, TrpA1^UAS.(B).cKa

(Nian et al., 2019)

mir-263b^Δ/mir-263b^Δ is a non-enhancer of axon collateral | adult stage phenotype of Scer\GAL4^Pdf.PU, TrpA1^UAS.(B).cKa

(Nian et al., 2019)

NOT Suppressor of

Statement

Reference

mir-263b^Δ/mir-263b^Δ is a non-suppressor of s-LNv Pdf neuron phenotype of Scer\GAL4^Pdf.PU, TrpA1^UAS.(B).cKa

(Nian et al., 2019)

mir-263b^Δ/mir-263b^Δ is a non-suppressor of axon collateral | adult stage phenotype of Scer\GAL4^Pdf.PU, TrpA1^UAS.(B).cKa

(Nian et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of interommatidial bristles seen in bft^Δ263a/bft²⁴ flies is significantly enhanced if the flies are also carrying mir-263b^Δ/Df(3L)X-21.2.

(Hilgers et al., 2010)

Xenogenetic Interactions

Statement

Reference

Acute activation of Pdf-positive neurons by expressing TrpA1^UAS.(B).cKa under the control of Scer\GAL4^Pdf.PU and temperature shift to 30[o]C at ZT12 leads to increased complexity of sLNv dorsal projections at Z14; the same is observed in a mir-263b^Δ/mir-263b^Δ background.

(Nian et al., 2019)

Complementation and Rescue Data

Partially rescued by

mir-263b^Δ-G4/mir-263b^Δ is partially rescued by mir-263b^UAS.cSa/Scer\GAL4^{mir-263b-Δ-G4}

(Nian et al., 2019)

mir-263b^Δ-G4/mir-263b^Δ is partially rescued by Scer\GAL4^Pdf.PU/mir-263b^UAS.cSa

(Nian et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58903

w^*; TI{TI}mir-263b^Δ

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

miR-263b KO

(Iftikhar et al., 2019)

miR-263b^KO

(Nian et al., 2019)

miR-263b^Δ

(Kim et al., 2017)

mir-263b^Δ

Δ263b

(Hilgers et al., 2010)

ΔmiR-263b

(Eyk et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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