FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\mir-bftΔ263a
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General Information
Symbol
Dmel\mir-bftΔ263a
Species
D. melanogaster
Name
FlyBase ID
FBal0257039
Feature type
allele
Associated gene
Dmel\mir-bft
Associated Insertion(s)
TI{TI}mir-bftΔ263a
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)
Mutagen
recombination
(Hilgers et al., 2010)
ends-out gene targeting
(Hilgers et al., 2010)
Progenitor genotype
Associated Insertion(s)
TI{TI}mir-bftΔ263a
Cytology
Description

350bp deletion that removes the mir-263a miRNA hairpin (which is encoded by bft). The miRNA hairpin has been replaced by a mini-w marker.

(Hilgers et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      bftΔ263a homozygous adults present an impaired immune response, as shown by the increased load of internal commensal bacteria and by the decreased survival upon bacterial infection with Pseudomonas aeruginosa, as compared to controls; the adult posterior midgut of these animals are significantly shorter and wider, frequently exhibit a multi-layered epithelium and evidence of anoikis of enterocytes, as shown by delamination and the presence of large lysosomes, and exhibit significant increases in the number of intestinal stem cells, which undergo symmetric cell divisions, in the number of mitotic cells at 14 days (but not at 7 days) post eclosion, in the transverse cell area (but not in the transverse nuclear area) and cell volume of enterocytes, as compared to controls.

      bftΔ263a homozygous mutant clones within a mosaic adult midgut present significant increases in the numbers of cells and intestinal stem cells, and also induce a cell non-autonomous increase in the numbers of mitotic cells and intestinal stem cells, as compared to control clones.

      The adult posterior midguts of bftΔ263a/bft24 transheterozygotes display a significant increase in the number of mitotic cells at 14 days post eclosion, as compared to controls.

      bftΔ263a/bftΔ263a-G4 transheterozygotes exhibit an overall increase in the gut pH and a significant increase in the number of mitotic cells in the adult posterior midgut at 14 days post eclosion, as compared to controls; these adult posterior midguts also exhibit a significant decrease in the peritrophic matrix thickness, which becomes significantly thicker upon the additional expression of bftScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 allele, where it replaces the bft hairpin sequence, as compared to controls.

      (Kim et al., 2017)

      Homozygous and bftΔ263a/Df(2L)BSC323 females show a significant decrease in median lifespan compared to controls.

      (Chen et al., 2014)

      Homozygous and bftΔ263a/bft24 flies show loss of approximately 80% of interommatidial bristles.

      bftΔ263a/bftΔ263a-G4 flies show loss of interommatidial bristles.

      The mutant retina appears normal in homozygous flies at 24 hours after puparium formation (APF). However, the majority of bristle shaft progenitor cells are missing in the mutant retina at 40 hours APF. Apoptotic nuclei corresponding to the bristle shaft cells are seen in the mutant retina at 35 hours APF.

      (Hilgers et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      mir-bftΔ263a/mir-bftΔ263a-G4 has adult midgut phenotype, suppressible by Scer\GAL4mir-bft-Δ263a-G4/NachGD2039

      (Kim et al., 2017)

      mir-bftΔ263a/mir-bftΔ263a-G4 has adult posterior midgut epithelium phenotype, suppressible by Scer\GAL4mir-bft-Δ263a-G4/NachGD2039

      (Kim et al., 2017)

      mir-bftΔ263a has adult midgut phenotype, suppressible | partially by NachJF02566/Scer\GAL4NP0001

      (Kim et al., 2017)

      mir-bftΔ263a has adult midgut phenotype, suppressible by Scer\GAL4NP0001/NachGD2039

      (Kim et al., 2017)

      mir-bftΔ263a has adult posterior midgut epithelium phenotype, suppressible | partially by NachJF02566/Scer\GAL4NP0001

      (Kim et al., 2017)

      mir-bftΔ263a has adult posterior midgut epithelium phenotype, suppressible by Scer\GAL4NP0001/NachGD2039

      (Kim et al., 2017)

      mir-bftΔ263a has adult posterior midgut epithelium phenotype, suppressible by Scer\GAL4NP0001/ppk6JF01919

      (Kim et al., 2017)

      mir-bftΔ263a has adult midgut phenotype, suppressible by Scer\GAL4NP0001/ppk16JF01931

      (Kim et al., 2017)

      mir-bftΔ263a has adult midgut phenotype, suppressible by Scer\GAL4NP0001/ppk28JF02153

      (Kim et al., 2017)

      mir-bftΔ263a has adult posterior midgut epithelium phenotype, suppressible by Scer\GAL4NP0001/ppk16JF01931

      (Kim et al., 2017)

      mir-bftΔ263a has adult posterior midgut epithelium phenotype, suppressible by Scer\GAL4NP0001/ppk28JF02153

      (Kim et al., 2017)

      mir-bftΔ263a/mir-bftΔ263a-G4 has adult midgut phenotype, suppressible by Scer\GAL4mir-bft-Δ263a-G4/NachJF02566

      (Kim et al., 2017)

      mir-bftΔ263a/mir-bftΔ263a-G4 has adult posterior midgut epithelium phenotype, suppressible by Scer\GAL4mir-bft-Δ263a-G4/NachJF02566

      (Kim et al., 2017)

      mir-bft24/mir-bftΔ263a has interommatidial bristle phenotype, suppressible by Scer\GAL4mir-263b-Δ-G4/mir-263bUAS.DsRed2

      (Hilgers et al., 2010)

      mir-bftunspecified/mir-bftΔ263a has interommatidial bristle phenotype, suppressible | partially by hid05014/W[+]

      (Hilgers et al., 2010)

      mir-bftunspecified/mir-bftΔ263a has interommatidial bristle phenotype, suppressible | partially by W[+]/hid1

      (Hilgers et al., 2010)

      mir-bftunspecified/mir-bftΔ263a has interommatidial bristle phenotype, suppressible by hidGD1673/Scer\GAL4mir-263b-Δ-G4

      (Hilgers et al., 2010)
      Enhancer of
      Statement
      Reference

      mir-bftΔ263a is an enhancer of eye phenotype of hidAla5.GMR

      (Hilgers et al., 2010)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of either NachJF02566 or NachGD2039 suppresses the increased number of mitotic cells in the adult posterior midgut exhibited by bftΔ263a/bftΔ263a-G4 transheterozygotes (expression under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence) or bftΔ263a homozygotes (expression under the control of Scer\GAL4Myo31DF-NP0001); the expression of NachJF02566 under the control of Scer\GAL4bft-Δ263a-G4 also suppresses the overall increase in the gut pH and the decreased thickness of the adult posterior midgut peritrophic matrix of bftΔ263a/bftΔ263a-G4 heterozygotes, even leading to its significant increase in thickness as compared to wild-type controls.

      The increased number of mitotic cells observed in the adult posterior midgut of bftΔ263a homozygotes is suppressed by the expression of either ppk6JF01919, ppk16JF01931 or ppk28JF02153, under the control of Scer\GAL4Myo31DF-NP0001.

      (Kim et al., 2017)

      The loss of interommatidial bristles seen in bftΔ263a/bft24 flies is significantly enhanced if the flies are also carrying mir-263bΔ/Df(3L)X-21.2.

      Expression of mir-263bScer\UAS.T:Disc\RFP-DsRed2 under the control of Scer\GAL4mir-263b-Δ-G4 rescues the loss of interommatidial bristles which is seen in bftΔ263a/bft24 flies.

      W05014/+ and W1/+ each partially suppress the loss of interommatidial bristles which is seen in bftΔ263a/bftunspecified flies.

      Expression of WGD1673 under the control of Scer\GAL4mir-263b-Δ-G4 strongly suppresses the loss of interommatidial bristles which is seen in bftΔ263a/bftunspecified flies.

      (Hilgers et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      mir-bftΔ263a/mir-bftΔ263a-G4 is partially rescued by Scer\GAL4mir-bft-Δ263a-G4/mir-bftUAS.pLUC

      (Kim et al., 2017)

      mir-bft24/mir-bftΔ263a is partially rescued by mir-bftUAS.DsRed2

      (Hilgers et al., 2010)
      Not rescued by

      mir-bftΔ263a is not rescued by Scer\GAL4NP0001/mir-bftUAS.pLUC

      (Kim et al., 2017)
      Comments

      The expression of bftScer\UAS.T:Ppyr\LUC driven by Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 allele, where it replaces the bft hairpin sequence, suppresses the overall increase in the gut pH, and suppresses both the increased number of mitotic cells and the decreased thickness of the peritrophic matrix in the posterior midgut of bftΔ263a/bftΔ263a-G4 transheterozygous mutant adults, even leading to a significant increase in the peritrophic matrix thickness as compared to wild-type controls.

      The expression of bftScer\UAS.T:Ppyr\LUC driven by Scer\GAL4Myo31DF-NP0001 does not significantly affect (it does not rescue or enhance) the increased number of mitotic cells observed in the adult posterior midgut of bftΔ263a homozygotes.

      (Kim et al., 2017)

      Leaky expression of bftScer\UAS.T:Disc\RFP-DsRed2 in the absence of a Scer\GAL4 driver partially rescues the loss of interommatidial bristles which is seen in bftΔ263a/bft24 flies.

      (Hilgers et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      bftΔ263a
      miR-263aΔ
      (Kim et al., 2017)
      mir-263aKO
      (Aw et al., 2017)
      mir-bftΔ263a
      Δ263a
      (Hilgers et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)