FB2021_01, released Feb 18, 2021

Allele: Dmel\mir-bftΔ263a-G4

Open Close
General Information
Symbol
Dmel\mir-bftΔ263a-G4
Species
D. melanogaster
Name
FlyBase ID
FBal0257040
Feature type
allele
Associated gene
Dmel\mir-bft
Associated Insertion(s)
TI{GAL4}mir-bftΔ263a-G4
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
recombination
(Hilgers et al., 2010)
gene targeting by homologous recombination
(Hilgers et al., 2010)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      Sequence encoding the mir-263a miRNA hairpin (which is encoded by bft) has been replaced by sequence encoding a GAL4 driver and a mini-w marker. This allows for expression of GAL4 under the control of the endogenous bft promoter.

      (Hilgers et al., 2010)
      Insertion components
      TI{GAL4}mir-bftΔ263a-G4
      Product class / Tool use(s)
      Encoded product / tool
      GAL4
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cystic fibrosis
      CEA with mir-bftΔ263a
      (Kim et al., 2017)
      CEA with mir-bft24
      (Kim et al., 2017)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      bftΔ263a/bftΔ263a-G4 transheterozygotes exhibit an overall increase in the gut pH and a significant increase in the number of mitotic cells in the adult posterior midgut at 14 days post eclosion, as compared to controls; these adult posterior midguts also exhibit a significant decrease in the peritrophic matrix thickness, which becomes significantly thicker upon the additional expression of bftScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence, as compared to controls.

      (Kim et al., 2017)

      bftΔ263a-G4/bft24 and bftΔ263a/bftΔ263a-G4 flies show loss of interommatidial bristles.

      (Hilgers et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of either NachJF02566 or NachGD2039 suppresses the increased number of mitotic cells in the adult posterior midgut exhibited by bftΔ263a/bftΔ263a-G4 transheterozygotes (expression under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence) or bftΔ263a homozygotes (expression under the control of Scer\GAL4Myo31DF-NP0001); the expression of NachJF02566 under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence, also suppresses the overall increase in the gut pH and the decreased thickness of the adult posterior midgut peritrophic matrix of bftΔ263a/bftΔ263a-G4 heterozygotes, even leading to its significant increase in thickness as compared to wild-type controls.

      (Kim et al., 2017)

      Expression of thScer\UAS.cHa under the control of Scer\GAL4bft-Δ263a-G4 suppresses the loss of interommatidial bristles which is seen in bftΔ263a-G4/bftunspecified flies.

      (Hilgers et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4bft-Δ263a-G4 suppresses the loss of interommatidial bristles which is seen in bftΔ263a-G4/bftunspecified flies.

      (Hilgers et al., 2010)
      Complementation and Rescue Data
      Rescued by

      mir-bft24/mir-bftΔ263a-G4 is rescued by Scer\GAL4mir-bft-Δ263a-G4/mir-bftUAS.DsRed2

      (Hilgers et al., 2010)
      Partially rescued by

      mir-bftΔ263a/mir-bftΔ263a-G4 is partially rescued by Scer\GAL4mir-bft-Δ263a-G4/mir-bftUAS.pLUC

      (Kim et al., 2017)
      Comments

      The expression of bftScer\UAS.T:Ppyr\LUC driven by Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence, suppresses the overall increase in the gut pH, and suppresses both the increased number of mitotic cells and the decreased thickness of the peritrophic matrix in the posterior midgut of bftΔ263a/bftΔ263a-G4 transheterozygous mutant adults, even leading to a significant increase in the peritrophic matrix thickness as compared to wild-type controls.

      (Kim et al., 2017)

      Expression of bftScer\UAS.T:Disc\RFP-DsRed2 under the control of Scer\GAL4bft-Δ263a-G4 rescues the loss of interommatidial bristles which is seen in bftΔ263a-G4/bft24 flies.

      (Hilgers et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      G4KO
      (Aw et al., 2017)
      bftΔ263a-G4
      miR-263aΔ-Gal4
      (Kim et al., 2017)
      mir-263aGal4KO
      (Aw et al., 2017)
      mir-bftΔ263a-G4
      Δ263a-G4
      (Hilgers et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)