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General Information
Symbol
Dmel\mir-bftΔ263a-G4
Species
D. melanogaster
Name
FlyBase ID
FBal0257040
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Sequence encoding the mir-263a miRNA hairpin (which is encoded by bft) has been replaced by sequence encoding a GAL4 driver and a mini-w marker. This allows for expression of GAL4 under the control of the endogenous bft promoter.

Insertion components
TI{GAL4}mir-bftΔ263a-G4
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

bftΔ263a/bftΔ263a-G4 transheterozygotes exhibit an overall increase in the gut pH and a significant increase in the number of mitotic cells in the adult posterior midgut at 14 days post eclosion, as compared to controls; these adult posterior midguts also exhibit a significant decrease in the peritrophic matrix thickness, which becomes significantly thicker upon the additional expression of bftScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence, as compared to controls.

bftΔ263a-G4/bft24 and bftΔ263a/bftΔ263a-G4 flies show loss of interommatidial bristles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The expression of either NachJF02566 or NachGD2039 suppresses the increased number of mitotic cells in the adult posterior midgut exhibited by bftΔ263a/bftΔ263a-G4 transheterozygotes (expression under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence) or bftΔ263a homozygotes (expression under the control of Scer\GAL4Myo31DF-NP0001); the expression of NachJF02566 under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence, also suppresses the overall increase in the gut pH and the decreased thickness of the adult posterior midgut peritrophic matrix of bftΔ263a/bftΔ263a-G4 heterozygotes, even leading to its significant increase in thickness as compared to wild-type controls.

Expression of thScer\UAS.cHa under the control of Scer\GAL4bft-Δ263a-G4 suppresses the loss of interommatidial bristles which is seen in bftΔ263a-G4/bftunspecified flies.

Xenogenetic Interactions
Statement
Reference

Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4bft-Δ263a-G4 suppresses the loss of interommatidial bristles which is seen in bftΔ263a-G4/bftunspecified flies.

Complementation and Rescue Data
Comments

The expression of bftScer\UAS.T:Ppyr\LUC driven by Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence, suppresses the overall increase in the gut pH, and suppresses both the increased number of mitotic cells and the decreased thickness of the peritrophic matrix in the posterior midgut of bftΔ263a/bftΔ263a-G4 transheterozygous mutant adults, even leading to a significant increase in the peritrophic matrix thickness as compared to wild-type controls.

Expression of bftScer\UAS.T:Disc\RFP-DsRed2 under the control of Scer\GAL4bft-Δ263a-G4 rescues the loss of interommatidial bristles which is seen in bftΔ263a-G4/bft24 flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
bftΔ263a-G4
miR-263aΔ-Gal4
mir-263aGal4KO
mir-bftΔ263a-G4
Name Synonyms
Secondary FlyBase IDs
    References (4)