FlyBase Allele Report: Scer\GAL4[mir-bft-Δ263a-G4]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Scer\GAL4^{mir-bft-Δ263a-G4}

General Information

Symbol

Scer\GAL4^{mir-bft-Δ263a-G4}

Species

S. cerevisiae

Name

FlyBase ID

FBal0257046

Feature type

allele

Associated gene

Associated Insertion(s)

TI{GAL4}mir-bft^Δ263a-G4

Carried in Construct

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

(Hilgers et al., 2010)

gene targeting by homologous recombination

(Hilgers et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}mir-bft^Δ263a-G4

Cytology

Description

Sequence encoding the mir-263a miRNA hairpin (which is encoded by bft) has been replaced by sequence encoding a GAL4 driver and a mini-w marker. This allows for expression of GAL4 under the control of the endogenous bft promoter.

(Hilgers et al., 2010)

Allele components

Component

Use(s)

Inserted element

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

interommatidial bristle

(Hilgers et al., 2010)

adult CNS glial cell

(Aw et al., 2017)

neuron | subset of adult brain

(Aw et al., 2017)

Additional Information

Statement

Reference

ScerGAL4^{bft-Δ263a-G4} drives expression in mostly in repo positive adult CNS glial cells, less than 2% of expression is seen in elav positive neurons in the brain.

(Aw et al., 2017)

Reporter expression is detected in the shaft cells of interommatidial bristles.

(Hilgers et al., 2010)

Marker for

Reflects expression of

(Hilgers et al., 2010)

(Aw et al., 2017)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

peritrophic membrane | adult stage, with mir-bft^Δ263a/mir-bft^Δ263a-G4, mir-bft^UAS.pLUC

(Kim et al., 2017)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^{mir-bft-Δ263a-G4}/Nach^GD2039 is a suppressor of increased occurrence of cell division | adult stage phenotype of mir-bft^Δ263a/mir-bft^Δ263a-G4

(Kim et al., 2017)

Scer\GAL4^{mir-bft-Δ263a-G4}/Nach^JF02566 is a suppressor of increased occurrence of cell division | adult stage phenotype of mir-bft^Δ263a/mir-bft^Δ263a-G4

(Kim et al., 2017)

Scer\GAL4^{mir-bft-Δ263a-G4}/Diap1^UAS.cHa is a suppressor of visible phenotype of mir-bft^unspecified/mir-bft^Δ263a-G4

(Hilgers et al., 2010)

Scer\GAL4^{mir-bft-Δ263a-G4}/BacA\p35^UAS.cHa is a suppressor of visible phenotype of mir-bft^unspecified/mir-bft^Δ263a-G4

(Hilgers et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^{mir-bft-Δ263a-G4}/Nach^JF02566 is a suppressor of adult midgut phenotype of mir-bft^Δ263a/mir-bft^Δ263a-G4

(Kim et al., 2017)

Scer\GAL4^{mir-bft-Δ263a-G4}/Nach^JF02566 is a suppressor of adult posterior midgut epithelium phenotype of mir-bft^Δ263a/mir-bft^Δ263a-G4

(Kim et al., 2017)

Scer\GAL4^{mir-bft-Δ263a-G4}/Nach^GD2039 is a suppressor of adult midgut phenotype of mir-bft^Δ263a/mir-bft^Δ263a-G4

(Kim et al., 2017)

Scer\GAL4^{mir-bft-Δ263a-G4}/Nach^GD2039 is a suppressor of adult posterior midgut epithelium phenotype of mir-bft^Δ263a/mir-bft^Δ263a-G4

(Kim et al., 2017)

Scer\GAL4^{mir-bft-Δ263a-G4}/Diap1^UAS.cHa is a suppressor of interommatidial bristle phenotype of mir-bft^unspecified/mir-bft^Δ263a-G4

(Hilgers et al., 2010)

Scer\GAL4^{mir-bft-Δ263a-G4}/BacA\p35^UAS.cHa is a suppressor of interommatidial bristle phenotype of mir-bft^unspecified/mir-bft^Δ263a-G4

(Hilgers et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{mir-bft-Δ263a-G4}/mir-bft^UAS.DsRed2 rescues mir-bft²⁴/mir-bft^Δ263a-G4

(Hilgers et al., 2010)

Partially rescues

Scer\GAL4^{mir-bft-Δ263a-G4}/mir-bft^UAS.pLUC partially rescues mir-bft^Δ263a/mir-bft^Δ263a-G4

(Kim et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^{bft-Δ263a-G4}

Scer\GAL4^{mir-bft-Δ263a-G4}

miR-263a-Gal4

(Hilgers et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections