FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\mir-bftUAS.pLUC
Open Close
General Information
Symbol
Dmel\mir-bftUAS.pLUC
Species
D. melanogaster
Name
FlyBase ID
FBal0277351
Feature type
allele
Associated gene
Dmel\mir-bft
Associated Insertion(s)
Carried in Construct
P{UAS-LUC-mir-bft.T}
Key Links
Transgenic product class
wild_type
(Bejarano et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bejarano et al., 2012)
Mutagen
in vitro construct
(Bejarano et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-LUC-mir-bft.T}
Cytology
Description

UAS regulatory sequences drive expression of Ppyr\LUC followed by a genomic fragment containing the bft pre-miRNA hairpin.

(Bejarano et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mir-bft
Tagged with
pLUC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  cystic fibrosis
      modeled by mir-bftΔ263a, mir-bftΔ263a-G4
      (Kim et al., 2017)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of bftScer\UAS.T:Ppyr\LUC driven by Scer\GAL4Myo31DF-NP0001 leads to a significant increase in the number of mitotic cells in the adult posterior midgut, as compared to controls.

      bftΔ263a/bftΔ263a-G4 transheterozygotes expressing bftScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence, present a significant increase in the thickness of the peritrophic matrix, as compared to controls.

      (Kim et al., 2017)

      Expression of bftScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4sd-SG29.1 results in the wing consisting of a large blister. Hairs on the wing margin are irregular.

      (Bejarano et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4mir-bft-Δ263a-G4/mir-bftUAS.pLUC partially rescues mir-bftΔ263a/mir-bftΔ263a-G4

      (Kim et al., 2017)
      Fails to rescue

      Scer\GAL4NP0001/mir-bftUAS.pLUC fails to rescue mir-bftΔ263a

      (Kim et al., 2017)
      Comments

      The expression of bftScer\UAS.T:Ppyr\LUC driven by Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence, suppresses the overall increase in the gut pH, and suppresses both the increased number of mitotic cells and the decreased thickness of the peritrophic matrix in the posterior midgut of bftΔ263a/bftΔ263a-G4 transheterozygous mutant adults, even leading to a significant increase in the peritrophic matrix thickness as compared to wild-type controls.

      The expression of bftScer\UAS.T:Ppyr\LUC driven by Scer\GAL4Myo31DF-NP0001 leads to an increase in the number of mitotic cells in the adult posterior midgut as compared to controls, but it does not significantly affect (it does not rescue or enhance) the increased number of mitotic cells observed in the adult posterior midgut of bftΔ263a homozygotes.

      (Kim et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      bftScer\UAS.T:Ppyr\LUC
      bftUAS.pLUC
      mir-bftUAS.pLUC
      Name Synonyms
      Secondary FlyBase IDs
        References (3)