FlyBase Allele Report: Dmel\tsr[HMS00534]

General Information

Symbol

Dmel\tsr^HMS00534

Species

D. melanogaster

Name

FlyBase ID

FBal0257222

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00534}

Also Known As

UAS-tsr-RNAi, UAS-tsr^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00534}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00534

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of nemaline myopathy 7

CEC

(Christophers et al., 2024)

CEA

(Balakrishnan et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of nemaline myopathy 7

is ameliorated by PI31^UAS.Tag:HA

(Balakrishnan et al., 2020)

is exacerbated by Prosβ6^1.B.UAS

(Balakrishnan et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

I band | larval stage, with Scer\GAL4^Mhc.PW

(Balakrishnan et al., 2020)

larval s-LNv neuron, with Scer\GAL4^P2.4.Pdf

(Liu et al., 2024)

larval somatic muscle cell | larval stage, with Scer\GAL4^Mhc.PW

(Balakrishnan et al., 2020)

larval somatic muscle cell | second instar larval stage, with Scer\GAL4^Mef2.PU

(Balakrishnan et al., 2020)

myotube | larval stage, with Scer\GAL4^Mhc.PW

(Balakrishnan et al., 2020)

myotube | second instar larval stage, with Scer\GAL4^Mef2.PU

(Balakrishnan et al., 2020)

neuron projection | larval stage, with Scer\GAL4^P2.4.Pdf

(Liu et al., 2024)

sarcomere | decreased number | second instar larval stage, with Scer\GAL4^Mhc.PW

(Balakrishnan et al., 2020)

sarcomere | larval stage, with Scer\GAL4^Mhc.PW

(Balakrishnan et al., 2020)

sarcomere | second instar larval stage, with Scer\GAL4^Mef2.PU

(Balakrishnan et al., 2020)

Detailed Description

Statement

Reference

Expressing tsr^HMS00534 under the control of Scer\GAL4^Mef2.PU leads to larvae showing disruption of muscle fiber structure and sarcomere organization.

Expressing tsr^HMS00534 under the control of Scer\GAL4^Mhc.PW disrupts the structure of larval muscles (starting at the second instar stage, which increased throughout the third instar stage), including disruption of the sarcomeric structure, decrease in sarcomere number with a concomitant increase in sarcomere length, and fewer sarcomeric F-actin bands; at the poles of defective muscles, the Z discs appear to be crosslinked by long actin filaments that are different sizes and disarrayed, instead of actin filaments of a specific length. These muscle defects correlate well with a reduction in larval crawling ability (starting at the second instar stage).

(Balakrishnan et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^Mhc.PW, tsr^HMS00534 has abnormal locomotor behavior | larval stage | progressive phenotype, non-enhanceable by Prosβ6^1.B.UAS, Scer\GAL4^Mhc.PW

(Balakrishnan et al., 2020)

Suppressed by