FlyBase Allele Report: Dmel\Nlg2[KO70]

General Information

Symbol

Dmel\Nlg2^KO70

Species

D. melanogaster

Name

FlyBase ID

FBal0258113

Feature type

allele

Associated gene

Dmel\Nlg2

Associated Insertion(s)

TI{TI}Nlg2^KO70

Carried in Construct

Also Known As

dnlg2^KO70

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Sun et al., 2011)

FLPase

(Sun et al., 2011)

I-SceI

(Sun et al., 2011)

Progenitor genotype

Associated Insertion(s)

TI{TI}Nlg2^KO70

Cytology

Description

A 2kb fragment of Nlg2 from the start codon to the middle of the first intron has been replaced by a w^+mW.hs marker.

(Sun et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autism spectrum disorder

CEA with Nlg3^KO127

(Guangming et al., 2023)

CEA

(Sun et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of autism spectrum disorder

is ameliorated by tsr^S3A.UAS.EGFP

(Sun et al., 2023)

is ameliorated by tsr^UAS.EGFP

(Sun et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Nlg2^KO70 mutant adults do not show adult locomotor activity defects.

(Wu et al., 2018)

Nlg2^KO70 homozygous mutant adults do not show any defects in the medulla columnar restriction of the axonal branches of L4 lamina neurons.

(Liu et al., 2017)

The neuromuscular junctions of Nlg2^KO70 third instar larvae exhibit a significant decrease in the number of synaptic boutons.

(Tu et al., 2017)

The number of boutons at the neuromuscular junction is significantly reduced compared to wild type in mutant third instar larvae.

Homozygous third instar larval show a reduction in EJP amplitude at the neuromuscular junction compared to controls. mEJP amplitude is not significantly altered. Quantal content is significantly reduced.

(Chen et al., 2012)

Homozygous neuroligin^KO70 mutants exhibit no detectable abnormalities in body size or morphology.

Neither the amplitude nor frequency of spontaneously occuring mEJPs are significantly different to wild-type in homozygous neuroligin^KO70 and heterozygous neuroligin^KO70/Df(2L)ED7007 mutants. In contrast, the amplitude of stimulus-evoked EJPs shows a small but significant increase in neuroligin^KO70 and neuroligin^KO70/Df(2L)ED7007 mutants. These effects are seen at both high (0.8mM) and low (0.2mM) calcium levels.

neuroligin^KO70 mutants exhibit a decrease in paired-pulse plasticity (where pairs of stimuli are given and the ratio between the amplitude generated from each pulse is calculated), indicating an increase in transmitter release probability.

Both the rise time and decay time of stimulus-evoked EJPs are significantly reduced in neuroligin^KO70 mutants. These reductions are observed in both 0.8mM and 0.2mM calcium backgrounds.

At a macromolecular level, synapses are formed with a wild-type pattern of innervations in neuroligin^KO70 mutants. However, the number of boutons is significantly reduced in these mutants compared to wild-type. The decreased number of boutons is associated with a significant decrease in the extent of synaptic arborization in neuroligin^KO70 mutants.

neuroligin^KO70 synapses exhibit smaller post-synaptic density zones and increased density of active zones. Individual T-bars appear to be normal in shape, but the total number of these structures per bouton is significantly increased.

neuroligin^KO70 mutants exhibit defects in post-synaptic organisation, specifically the reduction in length of post-synaptic density regions and a reduction in thickness of subsynaptic reticulum structures.

In early second-instar larvae, neuroligin^KO70 mutants show a significant reduction in bouton numbers compared with wild-type.

(Sun et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nlg2^KO70 has abnormal neuroanatomy phenotype, enhanceable by Nrx-1^Δ83

(Sun et al., 2011)

Nlg2^KO70 has abnormal neuroanatomy phenotype, enhanceable by Nrx-1[+]/Nrx-1^Δ83

(Sun et al., 2011)

Suppressed by

Statement

Reference

Nlg2^KO70 has abnormal neurophysiology | larval stage phenotype, suppressible by tsr^S3A.UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has abnormal neurophysiology | larval stage phenotype, suppressible | partially by tsr^S3E.UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has decreased rate of larval locomotory behavior phenotype, suppressible by tsr^S3A.UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has decreased rate of larval locomotory behavior phenotype, suppressible by tsr^UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has abnormal neurophysiology | larval stage phenotype, suppressible by tsr^UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by tsr^S3A.UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by tsr^UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

NOT suppressed by

Statement

Reference

Nlg2^KO70 has decreased rate of larval locomotory behavior phenotype, non-suppressible by tsr^S3E.UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Other

Statement

Reference

Nlg2^KO70, Nrx-1^Δ83 has lethal phenotype

(Sun et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nlg2^KO70 has bouton | second instar larval stage phenotype, enhanceable by Nrx-1^Δ83

(Sun et al., 2011)

Nlg2^KO70 has synapse | second instar larval stage phenotype, enhanceable by Nrx-1^Δ83

(Sun et al., 2011)

Nlg2^KO70 has bouton | second instar larval stage phenotype, enhanceable by Nrx-1[+]/Nrx-1^Δ83

(Sun et al., 2011)

Nlg2^KO70 has synapse | second instar larval stage phenotype, enhanceable by Nrx-1[+]/Nrx-1^Δ83

(Sun et al., 2011)

Suppressed by

Statement

Reference

Nlg2^KO70 has NMJ bouton | larval stage | decreased number phenotype, suppressible | partially by tsr^UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has filamentous actin | larval stage phenotype, suppressible | partially by tsr^S3A.UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has NMJ bouton | larval stage | decreased number phenotype, suppressible by tsr^S3A.UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by tsr^S3A.UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by tsr^UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

NOT suppressed by

Statement

Reference

Nlg2^KO70 has filamentous actin | larval stage phenotype, non-suppressible by tsr^UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Nlg2^KO70 has NMJ bouton | larval stage | decreased number phenotype, non-suppressible by tsr^S3E.UAS.EGFP/Scer\GAL4^C57

(Sun et al., 2023)

Enhancer of

Statement

Reference

neuroligin[+]/Nlg2^KO70 is an enhancer of bouton | second instar larval stage phenotype of Nrx-1^Δ83

(Sun et al., 2011)

neuroligin[+]/Nlg2^KO70 is an enhancer of synapse | second instar larval stage phenotype of Nrx-1^Δ83

(Sun et al., 2011)

Other

Statement

Reference

Nlg2^KO70, Scer\GAL4^C57, tsr^S3E.UAS.EGFP has filamentous actin | larval stage phenotype

(Sun et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous neuroligin^KO70;Nrx-1^Δ83 double mutants die during the second instar larval stage.

In early second-instar larvae, neuroligin^KO70;Nrx-1^Δ83 double mutants show a significant reduction in bouton numbers compared with wild-type as in neuroligin^KO70 single mutants. However, the double mutants appear to demonstrate more severe synaptic morphology defects than in the single mutants. Consistent with this, neuroligin^KO70;Nrx-1^Δ83 double mutants show more severe defects in locomotion than either single mutant.

Flies homozygous for neuroligin^KO70 and heterozygous for Nrx-1^Δ83 are viable and show more severe defects in neuromuscular morphology at both muscles 6/7 and muscle 4 than neuroligin^KO70 single mutants.

Nrx-1^Δ83 homozygous mutants in a neuroligin^KO70 heterozygous background exhibit more severe defects in neuromuscular morphology at both muscles 6/7 and muscle 4 than Nrx-1^Δ83 single mutants.

(Sun et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nlg2^KO70 is rescued by Scer\GAL4^da.PU/Nlg2^WT.UAS

(Tu et al., 2017)

Nlg2^KO70 is rescued by Nlg2^CTF.UAS/Scer\GAL4^da.PU

(Tu et al., 2017)

Nlg2^KO70 is rescued by Scer\GAL4^αTub84B.PL/Nlg2^UAS.cSa

(Chen et al., 2012)

Not rescued by

Nlg2^KO70 is not rescued by Scer\GAL4^da.PU/Nlg2^R598C.UAS

(Tu et al., 2017)

Nlg2^KO70 is not rescued by Scer\GAL4^da.PU/Nlg2^SF.UAS

(Tu et al., 2017)

Nlg2^KO70 is not rescued by Nlg2^UAS.cSa/Scer\GAL4^C57

(Chen et al., 2012)

Nlg2^KO70 is not rescued by Scer\GAL4^how-24B/Nlg2^UAS.cSa

(Chen et al., 2012)

Comments

Expression of Nlg2^Scer\UAS.cSa postsynaptically, under the control of either Scer\GAL4^C57 or Scer\GAL4^how-24B, does not rescue the reduction in bouton number seen at the neuromuscular junction in Nlg2^KO70 third instar larvae. However, expression of Nlg2^Scer\UAS.cSa in both neurons and muscles, under the control of Scer\GAL4^αTub84B.PL, restores bouton number at the neuromuscular junction in Nlg2^KO70 third instar larvae to wild-type levels.

Expression of Nlg2^Scer\UAS.cSa under the control of either Scer\GAL4^how-24B or Scer\GAL4^elav.PU does not rescue the reduced EJP amplitude seen at the neuromuscular junction in Nlg2^KO70 third instar larvae. Expression under the control of Scer\GAL4^αTub84B.PL rescues the EJP amplitude to wild type.

(Chen et al., 2012)

The increase in the number of T-bars seen per bouton in neuroligin^KO70 mutants is rescued upon expression of neuroligin^Scer\UAS.cSa by Scer\GAL4^how-24B.

Defects in post-synaptic organisation, specifically in the length of post-synaptic density regions and the thickness of subsynaptic reticulum structures are rescued upon expression of neuroligin^Scer\UAS.cSa post-synaptically under the control of Scer\GAL4^C57.

(Sun et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Nlg2^KO70

dnlg2⁷⁰

(Chen et al., 2012)

dnlg2^KO70

(Guangming et al., 2023, Sun et al., 2023, Wu et al., 2018, Xing et al., 2018, Liu et al., 2017, Tu et al., 2017, Chen et al., 2012)

neuroligin^KO70

Name Synonyms

Secondary FlyBase IDs

References (8)

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