FlyBase Allele Report: Dmel\Nrx-1[Δ83]

General Information

Symbol

Dmel\Nrx-1^Δ83

Species

D. melanogaster

Name

FlyBase ID

FBal0258010

Feature type

allele

Associated gene

Dmel\Nrx-1

Associated Insertion(s)

Carried in Construct

Also Known As

dnrx⁸³, dnrx^Δ83, nrx^Δ83

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Zeng et al., 2007)

Progenitor genotype

Nrx-1^d08766

(Zeng et al., 2007)

Cytology

Description

Mobilisation of P{XP}Nrx-1^d08766 by Δ2-3 transposase results in a 4066bp deletion.

(Zeng et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:22,470,310..22,474,366 [+]

Comment:

Reported as a 4066 bp deletion resulting from the imprecise excsion of P{XP}Nrx-1^d08766 extending from tgccgagtaacaccaacac to tatctcccatttctcatgac.

(Zeng et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autism spectrum disorder

CEA

(Guangming et al., 2023)

CEA with Nlg3^KO127

(Guangming et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of autism spectrum disorder

is exacerbated by Nlg4^KO10

(Guangming et al., 2023)

is ameliorated by Nrx-1^Δ174

(Guangming et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning

(Zeng et al., 2007)

abnormal locomotor behavior | late third instar larval stage

(Sun et al., 2009)

abnormal neuroanatomy | adult stage

(Liu et al., 2017)

abnormal neuroanatomy | adult stage (with Nrx-1²⁷³)

(Liu et al., 2017)

abnormal neurophysiology | adult stage

(Tian et al., 2013)

abnormal neurophysiology | late third instar larval stage

(Sun et al., 2009)

abnormal neurophysiology | third instar larval stage (with Nrx-1²⁷³)

(Li et al., 2015)

abnormal neurophysiology | third instar larval stage | conditional (with Nrx-1²⁷³)

(Li et al., 2015)

abnormal phototaxis | adult stage

(Tian et al., 2013)

fertile

(Sun et al., 2011, Zeng et al., 2007)

fertile (with Df(3R)Exel6191)

(Zeng et al., 2007)

short lived

(Zeng et al., 2007)

viable

(Sun et al., 2011, Zeng et al., 2007)

viable (with Df(3R)Exel6191)

(Zeng et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

Unlike in wild-type, the axon of L4 lamina neuron in Nrx-1^Δ83 homozygous as well as Nrx-1^Δ83/Nrx-1²⁷³ transheterozygous adults is not restricted to a single medulla column but often extends to neighboring columns, although the axons still terminate appropriately in the M4 layers.

(Liu et al., 2017)

Third instar neuromuscular junctions of Nrx-1²⁷³/Nrx-1^Δ83 mutants exhibit a significant Ca[2+]-concentration-dependent reduction in excitatory junction current (EJC) amplitude, and a small but significant reduction in steady-state quantal content, in response to a 1-Hz train of stimulation, as compared to wild type. In response to 40 Hz tetanic nerve stimulation, NMJs in these mutants show a marked activity-dependent reduction in the amplitude of EJCs under 0.5mM Ca[2+] conditions, show rapid synaptic depression with amplitude of evoked currents showing a steeper decline after 8 stimuli under 1.8mM Ca[2+] conditions, and show a significant reduction in steady state EJC and quantal content, but no significant difference in steady state miniature EJC (mEJC), as compared to wild type.

(Li et al., 2015)

Mutant flies show a reduced phototactic response to light compared to wild type. Mutant photoreceptors show approximately 10,000-fold light sensitivity compared to wild type. Mutant flies do not show a blue light-induced prolonged depolarization afterpotential.

Mutant photoreceptors have a normal endoplasmic reticulum structure at 65% of pupal development. However, at 79% of pupal development, the endoplasmic reticulum appears expanded.

(Tian et al., 2013)

neuroanatomy defective, enhanceable { neuroligin[KO70]/+ }

(Sun et al., 2011)

Nrx-1^Δ83 larvae display a severe defect in locomotion and a 49% decrease in bouton number at neuromuscular junctions.

Nrx-1^Δ83 neuromuscular junctions show a severe reduction in synaptic vesicle trafficking.

Nrx-1^Δ83/+ neuromuscular junctions show a small but significant reduction in synaptic vesicle trafficking.

Induced excitatory potential (EJP) amplitude at Nrx-1^Δ83 neuromuscular junctions is significantly decreased compared to wild type.

Nrx-1^Δ83/+ neuromuscular junctions show a small but significant reduction in EJP amplitude.

Both the spontaneous miniature excitatory potential (mEJP) amplitude and frequency at Nrx-1^Δ83 neuromuscular junctions are significantly increased compared to wild type.

(Sun et al., 2009)

Nrx-1^Δ83 and Nrx-1^Δ83/Df(3R)Exel6191 mutants are viable and fertile, with no obvious developmental defects.

Nrx-1^Δ83 mutants are short-lived. At 37 days, only 32.5% of Nrx-1^Δ83 mutants are alive, compared with 82.5% of wild-type and 79% of rescue flies (Scer\GAL4^elav-C155/+; Nrx-1^Scer\UAS.cZa/+; Nrx-1^Δ83).

Nrx-1^Δ83 and Nrx-1^Δ83/Df(3R)Exel6191 mutants exhibit fewer synapses than controls (approximately half).

Associative learning is reduced in Nrx-1^Δ83 mutants (testing for their ability to associate odors with a fructose reward in an individual-animal assay).

(Zeng et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nrx-1^Δ83 has abnormal neurophysiology | late third instar larval stage phenotype, enhanceable by CASK^X-307/CASK[+]

(Sun et al., 2009)

NOT Enhanced by

Statement

Reference

Nrx-1^Δ83 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Nlg4^KO10/Nlg4^KO10

(Liu et al., 2017)

Enhancer of

Statement

Reference

Nrx-1^Δ83 is an enhancer of abnormal neuroanatomy phenotype of Nlg2^KO70

(Sun et al., 2011)

Nrx-1[+]/Nrx-1^Δ83 is an enhancer of abnormal neuroanatomy phenotype of Nlg2^KO70

(Sun et al., 2011)

Nrx-1[+]/Nrx-1^Δ83 is an enhancer of abnormal neurophysiology | late third instar larval stage phenotype of CASK^X-307

(Sun et al., 2009)

NOT Enhancer of

Statement

Reference

Nrx-1^Δ83/Nrx-1^Δ83 is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Nlg4^KO10

(Liu et al., 2017)

Nrx-1²⁷³/Nrx-1^Δ83 is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Ephrin^HMS01289, Scer\GAL4^GMR31C06

(Liu et al., 2017)

Other

Statement

Reference

Nlg2^KO70, Nrx-1^Δ83 has lethal phenotype

(Sun et al., 2011)

CASK^X-307/CASK[+], Nrx-1^Δ83 has abnormal locomotor behavior | late third instar larval stage phenotype

(Sun et al., 2009)

CASK^X-307/CASK[+], Nrx-1^Δ83 has abnormal neurophysiology | late third instar larval stage phenotype

(Sun et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nrx-1^Δ83 has bouton | second instar larval stage phenotype, enhanceable by neuroligin[+]/Nlg2^KO70

(Sun et al., 2011)

Nrx-1^Δ83 has synapse | second instar larval stage phenotype, enhanceable by neuroligin[+]/Nlg2^KO70

(Sun et al., 2011)

Nrx-1^Δ83 has synaptic vesicle phenotype, enhanceable by CASK^X-307/CASK[+]

(Sun et al., 2009)

NOT Enhanced by

Statement

Reference

Nrx-1^Δ83 has lamina monopolar neuron L4 | adult stage phenotype, non-enhanceable by Nlg4^KO10/Nlg4^KO10

(Liu et al., 2017)

Nrx-1^Δ83 has axon | adult stage phenotype, non-enhanceable by Nlg4^KO10/Nlg4^KO10

(Liu et al., 2017)

Nrx-1^Δ83 has medulla | adult stage phenotype, non-enhanceable by Nlg4^KO10/Nlg4^KO10

(Liu et al., 2017)

Enhancer of

Statement

Reference

Nrx-1^Δ83 is an enhancer of bouton | second instar larval stage phenotype of Nlg2^KO70

(Sun et al., 2011)

Nrx-1^Δ83 is an enhancer of synapse | second instar larval stage phenotype of Nlg2^KO70

(Sun et al., 2011)

Nrx-1[+]/Nrx-1^Δ83 is an enhancer of bouton | second instar larval stage phenotype of Nlg2^KO70

(Sun et al., 2011)

Nrx-1[+]/Nrx-1^Δ83 is an enhancer of synapse | second instar larval stage phenotype of Nlg2^KO70

(Sun et al., 2011)

Nrx-1[+]/Nrx-1^Δ83 is an enhancer of synaptic vesicle phenotype of CASK^X-307

(Sun et al., 2009)

NOT Enhancer of

Statement

Reference

Nrx-1²⁷³/Nrx-1^Δ83 is a non-enhancer of axon | adult stage phenotype of Ephrin^HMS01289, Scer\GAL4^GMR31C06

(Liu et al., 2017)

Nrx-1²⁷³/Nrx-1^Δ83 is a non-enhancer of medulla | adult stage phenotype of Ephrin^HMS01289, Scer\GAL4^GMR31C06

(Liu et al., 2017)

Nrx-1^Δ83/Nrx-1^Δ83 is a non-enhancer of lamina monopolar neuron L4 | adult stage phenotype of Nlg4^KO10

(Liu et al., 2017)

Nrx-1^Δ83/Nrx-1^Δ83 is a non-enhancer of axon | adult stage phenotype of Nlg4^KO10

(Liu et al., 2017)

Nrx-1^Δ83/Nrx-1^Δ83 is a non-enhancer of medulla | adult stage phenotype of Nlg4^KO10

(Liu et al., 2017)

Nrx-1²⁷³/Nrx-1^Δ83 is a non-enhancer of lamina monopolar neuron L4 | adult stage phenotype of Ephrin^HMS01289, Scer\GAL4^GMR31C06

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The impaired columnar restriction of L4 lamina neuron axons (which frequently invade adjacent medulla columns) observed in adult flies expressing Ephrin^HMS01289 under the control of Scer\GAL4^GMR31C06 is not worsened further by combination with Nrx-1^Δ83/Nrx-1²⁷³ (which on its own also causes defects in columnar restriction of the L4 axons).

The frequency of L4 lamina neuron axons aberrantly invading adjacent medulla columns in adult Nlg4^KO10,Nrx-1^Δ83 double mutants is not significantly different from each of the single mutants.

(Liu et al., 2017)

Homozygous neuroligin^KO70;Nrx-1^Δ83 double mutants die during the second instar larval stage.

In early second-instar larvae, neuroligin^KO70;Nrx-1^Δ83 double mutants show a significant reduction in bouton numbers compared with wild-type as in neuroligin^KO70 single mutants. However, the double mutants appear to demonstrate more severe synaptic morphology defects than in the single mutants. Consistent with this, neuroligin^KO70;Nrx-1^Δ83 double mutants show more severe defects in locomotion than either single mutant.

Flies homozygous for neuroligin^KO70 and heterozygous for Nrx-1^Δ83 are viable and show more severe defects in neuromuscular morphology at both muscles 6/7 and muscle 4 than neuroligin^KO70 single mutants.

Nrx-1^Δ83 homozygous mutants in a neuroligin^KO70 heterozygous background exhibit more severe defects in neuromuscular morphology at both muscles 6/7 and muscle 4 than Nrx-1^Δ83 single mutants.

(Sun et al., 2011)

In contrast to either heterozygote alone, Nrx-1^Δ83/+, CASK^X-307/+ larvae display a marked defect in locomotion.

Similar to either heterozygote alone, Nrx-1^Δ83/+, CASK^X-307/+ larvae display no significant differences in bouton number at neuromuscular junctions compared to wild type.

Nrx-1^Δ83/+, CASK^X-307/+ neuromuscular junctions show a much more severe reduction in synaptic vesicle trafficking compared to either single heterozygote.

Nrx-1^Δ83/+, CASK^X-307/+ neuromuscular junctions show a more severe reduction in induced excitatory potential (EJP) amplitude compared to either single heterozygote.

In contrast to either heterozygote alone, Nrx-1^Δ83/+, CASK^X-307/+ neuromuscular junctions show a significant increase in the spontaneous miniature excitatory potential (mEJP) amplitude and frequency compared to wild type.

(Sun et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nrx-1^Δ83 is rescued by Scer\GAL4^GMR31C06/Nrx-1^UAS.cZa

(Liu et al., 2017)

Nrx-1²⁷³/Nrx-1^Δ83 is rescued by Scer\GAL4^RapGAP1-OK6/Nrx-1^UAS.cZa

(Li et al., 2015)

Nrx-1^Δ83 is rescued by Nrx-1^UAS.cZa/Scer\GAL4^GMR.PU

(Tian et al., 2013)

Nrx-1^Δ83 is rescued by Scer\GAL4^elav-C155/Nrx-1^UAS.cZa

(Zeng et al., 2007)

Partially rescued by

Nrx-1²⁷³/Nrx-1^Δ83 is partially rescued by Scer\GAL4^RapGAP1-OK6/Nrx-1^ΔC.UAS

(Li et al., 2015)

Nrx-1²⁷³/Nrx-1^Δ83 is partially rescued by Scer\GAL4^RapGAP1-OK6/Nrx-1^ΔPBD.UAS

(Li et al., 2015)

Not rescued by

Nrx-1^Δ83 is not rescued by Nrx-1^UAS.cZa/Scer\GAL4^c739

(Liu et al., 2017)

Nrx-1^Δ83 is not rescued by Scer\GAL4^GMR31C06/Nrx-1^{Δ1760-1813.UAS}

(Liu et al., 2017)

Nrx-1^Δ83 is not rescued by Scer\GAL4^Tab2-201Y/Nrx-1^UAS.cZa

(Zeng et al., 2007)

Comments

The defective columnar restriction of L4 lamina neuron axons (which frequently invade adjacent medulla columns) observed in Nrx-1^Δ83 mutant adults is fully rescued by expression of Nrx-1^Scer\UAS.cZa driven by Scer\GAL4^GMR31C06 (but not Scer\GAL4^c739). The impaired columnar restriction is also largely restored by Scer\GAL4^GMR31C06-controlled expression that is restricted to 48-60 hr after puparium formation (APF) time window using tub-Gal80[ts], while expression induced between 80-92 hr APF fails to rescue the defect.

Expression of Nrx-1^{Δ1760-1813.Scer\UAS} driven by Scer\GAL4^GMR31C06 however cannot rescue the L4 axonal targeting defects of Nrx-1^Δ83 mutants.

(Liu et al., 2017)

Expression of Nrx-1^Scer\UAS.cZa (but not Nrx-1^{ΔPBD.Scer\UAS} or Nrx-1^ΔC.Scer\UAS) under the control of Scer\GAL4^RapGAP1-OK6 rescues the rapid synaptic depression seen in third instar Nrx-1²⁷³/Nrx-1^Δ83 mutant NMJs under 1.8mM Ca[2+] conditions, and expression of Nrx-1^Scer\UAS.cZa or Nrx-1^{ΔPBD.Scer\UAS} (but not Nrx-1^ΔC.Scer\UAS) rescues the reduced mean EJCs, and the reduced quantal content at the steady state seen in third instar Nrx-1²⁷³/Nrx-1^Δ83 mutant NMJs under 1.8mM Ca[2+] conditions.

Expression of Nrx-1^{ΔPBD.Scer\UAS} or Nrx-1^Scer\UAS.cZa under the control of Scer\GAL4^RapGAP1-OK6 restores the decreased initial EJC amplitude and the reduced mean EJCs at the steady state observed in third instar Nrx-1²⁷³/Nrx-1^Δ83 mutant NMJs under 0.5mM Ca[2+] conditions.

Expression of Nrx-1^{ΔPBD.Scer\UAS} under the control of Scer\GAL4^RapGAP1-OK6 restores the decreased initial EJC amplitude but not the the reduced mean EJCs at the steady state observed in third instar Nrx-1²⁷³/Nrx-1^Δ83 mutant NMJs under 0.5mM Ca[2+] conditions.

(Li et al., 2015)

The reduced light sensitivity of Nrx-1^Δ83 photoreceptors is rescued by expression of Nrx-1^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PU.

(Tian et al., 2013)

Synapse number is rescued in Scer\GAL4^elav-C155/+; Nrx-1^Scer\UAS.cZa/+; Nrx-1^Δ83 flies.

Longevity is rescued in Scer\GAL4^elav-C155/+; Nrx-1^Scer\UAS.cZa/+; Nrx-1^Δ83 flies.

Associative learning is rescued inScer\GAL4^elav-C155/+; Nrx-1^Scer\UAS.cZa/+; Nrx-1^Δ83 flies.

(Zeng et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Dnrx^Δ83

(Han et al., 2020)

Nrx-1^Δ83

dnrx⁸³

(Zhao et al., 2024, Guangming et al., 2023, Wu et al., 2018, Xing et al., 2018, Rui et al., 2017)

dnrx^Δ83

(Liu et al., 2017, Sun et al., 2016, Sun et al., 2009)

nrx-1^Δ83

(Koper et al., 2012)

nrx^Δ83

(Tong et al., 2016, Li et al., 2015, Tian et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (15)

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