FlyBase Allele Report: Dmel\Nrx-1[UAS.cZa]

General Information

Symbol

Dmel\Nrx-1^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Zeng

FlyBase ID

FBal0321720

Feature type

allele

Associated gene

Dmel\Nrx-1

Associated Insertion(s)

Carried in Construct

P{UAS-Nrx-1.fl}

Also Known As

UAS-Neurexin, UAS-DNrx

Key Links

Transgenic product class

cDNA

(Zeng et al., 2007)

wild_type

(Zeng et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zeng et al., 2007)

wild_type

(Zeng et al., 2007)

Mutagen

in vitro construct

(Tian et al., 2013, Sun et al., 2009, Zeng et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Nrx-1.fl}

Cytology

Description

UASt regulatory sequences drive expression of full-length Nrx-1 coding sequences (derived from the LP14275 cDNA clone).

(Zeng et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Nrx-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav.PU

(Zweier et al., 2009)

Phenotype Manifest In

larval brain | larval stage, with Scer\GAL4^elav.PU

(Zweier et al., 2009)

NMJ bouton | larval stage, with Scer\GAL4^elav.PU

(Zweier et al., 2009)

presynaptic active zone | larval stage, with Scer\GAL4^elav.PU

(Zweier et al., 2009)

Detailed Description

Statement

Reference

Larvae with expression of Nrx-1^Scer\UAS.cZa driven by Scer\GAL4^elav.PU show significantly increased staining intensity of the presynaptic protein brp; this is dosage dependent, with two copies of Nrx-1^Scer\UAS.cZa resulting in further increases in intensity. At the neuromuscular junction, Scer\GAL4^elav.PU>Nrx-1^Scer\UAS.cZa larvae show a significant increase in bouton number (and boutons are smaller), increase in active zone number (per synaptic area) and increase in branches (per synaptic terminal), compared to controls.

(Zweier et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^GMR31C06/Nrx-1^UAS.cZa rescues Nrx-1^Δ83

(Liu et al., 2017)

Scer\GAL4^RapGAP1-OK6/Nrx-1^UAS.cZa rescues Nrx-1²⁷³/Nrx-1^Δ83

(Li et al., 2015)

Nrx-1^UAS.cZa/Scer\GAL4^GMR.PU rescues Nrx-1^Δ83

(Tian et al., 2013)

Scer\GAL4^elav-C155/Nrx-1^UAS.cZa rescues Nrx-1^Δ83

(Zeng et al., 2007)

Fails to rescue

Nrx-1^UAS.cZa/Scer\GAL4^c739 fails to rescue Nrx-1^Δ83

(Liu et al., 2017)

Scer\GAL4^Tab2-201Y/Nrx-1^UAS.cZa fails to rescue Nrx-1^Δ83

(Zeng et al., 2007)

Comments

The defective columnar restriction of L4 lamina neuron axons (which frequently invade adjacent medulla columns) observed in Nrx-1^Δ83 mutant adults is fully rescued by expression of Nrx-1^Scer\UAS.cZa driven by Scer\GAL4^GMR31C06 (but not Scer\GAL4^c739). The impaired columnar restriction is also largely restored by Scer\GAL4^GMR31C06-controlled expression that is restricted to 48-60 hr after puparium formation (APF) time window using tub-Gal80[ts], while expression induced between 80-92 hr APF fails to rescue the defect.

(Liu et al., 2017)

Expression of Nrx-1^Scer\UAS.cZa under the control of Scer\GAL4^RapGAP1-OK6 rescues the rapid synaptic depression, the reduced mean EJCs, and the reduced quantal content at the steady state seen in third instar Nrx-1²⁷³/Nrx-1^Δ83 mutant NMJs under 1.8mM Ca[2+] conditions.

Expression of Nrx-1^Scer\UAS.cZa under the control of Scer\GAL4^RapGAP1-OK6 restores the decreased initial EJC amplitude and the reduced mean EJCs at the steady state observed in third instar Nrx-1²⁷³/Nrx-1^Δ83 mutant NMJs under 0.5mM Ca[2+] conditions.

(Li et al., 2015)

The reduced light sensitivity of Nrx-1^Δ83 photoreceptors is rescued by expression of Nrx-1^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PU.

(Tian et al., 2013)

Synapse number is rescued in Scer\GAL4^elav-C155/+; Nrx-1^Scer\UAS.cZa/+; Nrx-1^Δ83 flies.

Longevity is rescued in Scer\GAL4^elav-C155/+; Nrx-1^Scer\UAS.cZa/+; Nrx-1^Δ83 flies.

Associative learning is rescued inScer\GAL4^elav-C155/+; Nrx-1^Scer\UAS.cZa/+; Nrx-1^Δ83 flies.

(Zeng et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

NRX^FL

(Li et al., 2015)

Nrx-1^Scer\UAS.cSa

Nrx-1^Scer\UAS.cTa

Nrx-1^Scer\UAS.cZa

Nrx-1^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sun

Saccharomyces cerevisiae UAS construct of Tian

Saccharomyces cerevisiae UAS construct of Zeng

Secondary FlyBase IDs

FBal0258009
FBal0282666
FBal0287943

References (10)

Report Sections

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