FlyBase Allele Report: Dmel\Nlg4[KO10]

General Information

Symbol

Dmel\Nlg4^KO10

Species

D. melanogaster

Name

FlyBase ID

FBal0325865

Feature type

allele

Associated gene

Dmel\Nlg4

Associated Insertion(s)

TI{TI}Nlg4^KO10

Carried in Construct

Also Known As

dnlg4^KO10

Key Links

Allele class

amorphic allele - molecular evidence

loss of function allele

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Zhang et al., 2017)

loss of function allele

(Liu et al., 2017)

Mutagen

ends-out gene targeting

(Liu et al., 2017, Zhang et al., 2017)

Progenitor genotype

Associated Insertion(s)

TI{TI}Nlg4^KO10

Cytology

Description

Described as a null mutant generated by ends-out technology.

(Liu et al., 2017)

A 3.8kb fragment of the Nlg4 gene (includes the first 5 coding exons) has been deleted and replaced with a w^+mW.hs marker that is flanked by loxP sites.

(Zhang et al., 2017)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autism spectrum disorder

modeled by Nrx-1^Δ83

(Guangming et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Nlg4^KO10 mutant adults do not show adult locomotor activity defects.

(Wu et al., 2018)

Unlike in wild-type, the axon of L4 lamina neuron in Nlg4^KO10 homozygous adults is not restricted to a single medulla column but often extends to neighboring columns.

(Liu et al., 2017)

Although the overall morphology of neuromuscular junctions (NMJs) in Nlg4^KO10 as well as Nlg4^KO10/Df(3R)ED6027 mutant third instar larvae appears unperturbed, the axon branches are significantly shortened and the number of boutons reduced. No defects in the number of presynaptic vesicles or T-bar morphology in Nlg4^KO10 NMJ boutons, but the bouton area per active zone is increased as is the single (but not overall) postsynaptic density length. Nlg4^KO10 mutants however show active zone structure defects: the presynaptic membrane is detached from the opposite postsynaptic membrane at several points and display invaginations. The area of subsynaptic reticulum per bouton is decreased.

Both Nlg4^KO10 homozygous and Nlg4^KO10/Df(3R)ED6027 larvae display small but significant decrease in the quantal content as well as the amplitude of evoked excitatory junction potentials (EJPs), but not the miniature EJPs (mEJPs). The frequency of mEJPs is dramatically increased in the mutants as compared with wild-type controls. Nlg4^KO10 and Nlg4^KO10/Df(3R)ED6027 mutant larvae also show reduced locomotion (quantified as distance crawled within 3-min time limit)

(Zhang et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Nlg4^KO10 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Nrx-1^Δ83/Nrx-1^Δ83

(Liu et al., 2017)

NOT Enhancer of

Statement

Reference

Nlg4^KO10/Nlg4^KO10 is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Nrx-1^Δ83

(Liu et al., 2017)

Nlg4^KO10/Nlg4^KO10 is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Ephrin^HMS01289, Scer\GAL4^GMR31C06

(Liu et al., 2017)

Suppressor of

Statement

Reference

Nlg4^KO10/Nlg4^KO10 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Dad^j1E4

(Zhang et al., 2017)

Other

Statement

Reference

Nlg4^KO10, tkv⁷/tkv[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Zhang et al., 2017)

Nlg4^KO10, wit^A12/wit[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Zhang et al., 2017)

Mad^8-2/Mad[+], Nlg4^KO10 has abnormal neuroanatomy | third instar larval stage phenotype

(Zhang et al., 2017)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Nlg4^KO10 has lamina monopolar neuron L4 | adult stage phenotype, non-enhanceable by Nrx-1^Δ83/Nrx-1^Δ83

(Liu et al., 2017)

Nlg4^KO10 has axon | adult stage phenotype, non-enhanceable by Nrx-1^Δ83/Nrx-1^Δ83

(Liu et al., 2017)

Nlg4^KO10 has medulla | adult stage phenotype, non-enhanceable by Nrx-1^Δ83/Nrx-1^Δ83

(Liu et al., 2017)

NOT Enhancer of

Statement

Reference

Nlg4^KO10/Nlg4^KO10 is a non-enhancer of lamina monopolar neuron L4 | adult stage phenotype of Nrx-1^Δ83

(Liu et al., 2017)

Nlg4^KO10/Nlg4^KO10 is a non-enhancer of axon | adult stage phenotype of Ephrin^HMS01289, Scer\GAL4^GMR31C06

(Liu et al., 2017)

Nlg4^KO10/Nlg4^KO10 is a non-enhancer of medulla | adult stage phenotype of Ephrin^HMS01289, Scer\GAL4^GMR31C06

(Liu et al., 2017)

Nlg4^KO10/Nlg4^KO10 is a non-enhancer of axon | adult stage phenotype of Nrx-1^Δ83

(Liu et al., 2017)

Nlg4^KO10/Nlg4^KO10 is a non-enhancer of medulla | adult stage phenotype of Nrx-1^Δ83

(Liu et al., 2017)

Nlg4^KO10/Nlg4^KO10 is a non-enhancer of lamina monopolar neuron L4 | adult stage phenotype of Ephrin^HMS01289, Scer\GAL4^GMR31C06

(Liu et al., 2017)

Suppressor of

Statement

Reference

Nlg4^KO10/Nlg4^KO10 is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Dad^j1E4

(Zhang et al., 2017)

Other

Statement

Reference

Nlg4^KO10, tkv⁷/tkv[+] has NMJ bouton | third instar larval stage phenotype

(Zhang et al., 2017)

Nlg4^KO10, wit^A12/wit[+] has NMJ bouton | third instar larval stage phenotype

(Zhang et al., 2017)

Mad^8-2/Mad[+], Nlg4^KO10 has NMJ bouton | third instar larval stage phenotype

(Zhang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of L4 lamina neuron axons aberrantly invading adjacent medulla columns in adult Nlg4^KO10,Nrx-1^Δ83 double mutants is not significantly different from each of the single mutants.

The impaired columnar restriction of L4 lamina neuron axons (which frequently invade adjacent medulla columns) observed in adult flies expressing Ephrin^HMS01289 under the control of Scer\GAL4^GMR31C06 is not worsened further by combination with Nlg4^KO10 in homozygous state - either of which on their own also causes defects in columnar restriction of the L4 axons.

(Liu et al., 2017)

Although none of the larvae heterozygous for any of the following alleles: Nlg4^KO10, tkv⁷, wit^A12 or Mad^8-2 display any significant difference in the number or size of boutons on neuromuscular junctions compared to controls, double heterozygotes for Nlg4^KO10 and tkv⁷ or wit^A12 or Mad^8-2 all show reduced bouton number and increased bouton size.

The synaptic overgrowth phenotype (increased number of boutons at neuromuscular junctions) characteristic for third instar larvae homozygous for Dad^j1E4 is completely suppressed by Nlg4^KO10 homozygosity and the resulting number of boutons per NMJ is comparable to Nlg4^KO10 single mutants and significantly lower than in wild-type controls.

(Zhang et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nlg4^KO10 is rescued by Scer\GAL4^oc.1.6/Nlg4^UAS.cLa

(Liu et al., 2017)

Nlg4^KO10 is rescued by Scer\GAL4^elav.PU/Nlg4^UAS.cZa

(Zhang et al., 2017)

Nlg4^KO10 is rescued by Scer\GAL4^RapGAP1-OK6/Nlg4^UAS.cZa

(Zhang et al., 2017)

Not rescued by

Nlg4^KO10 is not rescued by Scer\GAL4^C57/Nlg4^UAS.cZa

(Zhang et al., 2017)

Comments

The impaired columnar restriction of L4 lamina neuron axons (which frequently invade adjacent medulla columns) observed in Nlg4^KO10 mutant adult flies is rescued by expression of Nlg4^Scer\UAS.cLa under the control of Scer\GAL4^oc.1.6 in the mutant background.

(Liu et al., 2017)

The decreased number but increased size of boutons on neuromuscular junctions in Nlg4^KO10 mutant third instar larvae is fully rescued by expression of Nlg4^Scer\UAS.cZa if driven by either Scer\GAL4^elav.PU or Scer\GAL4^RapGAP1-OK6 but not Scer\GAL4^C57. Similarly, the functional neurotransmission defects (reduced quantal content, decreased amplitude of evoked excitatory junction potentials) along with the larval locomotor deficit are rescued by Scer\GAL4^elav.PU-driven expression of Nlg4^Scer\UAS.cZa but not if it is controlled by Scer\GAL4^C57.

(Zhang et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Nlg4^KO10

dnlg4^KO10

(Guangming et al., 2023, Wu et al., 2018, Liu et al., 2017, Zhang et al., 2017)

dnlg4^Δ10

(Liu et al., 2017)

nlg4^Δ10

(Guangming et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (4)

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