FlyBase Allele Report: Dmel\Mad[8-2]

General Information

Symbol

Dmel\Mad^8-2

Species

D. melanogaster

Name

FlyBase ID

FBal0052176

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

P{lacW}Mad^8-2

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Wiersdorff et al., 1996)

Mutagen

P-element activity

(Wiersdorff et al., 1996)

Progenitor genotype

Mad^B1

(Wiersdorff et al., 1996)

Associated Insertion(s)

P{lacW}Mad^8-2

Cytology

Description

Full-length P{lacW} insertion is inserted in the first intron of Mad.

(Chen et al., 2022)

Imprecise excision: P{lacW} sequences are retained at the site of the original insertion.

(Wiersdorff et al., 1996)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Wiersdorff et al., 1996)

Phenotype Manifest In

eye-antennal disc

(Wiersdorff et al., 1996)

Detailed Description

Statement

Reference

Third instar larvae heterozygous for Mad^8-2 show no significant differences in the number or size of neuromuscular junction boutons compared to wild-type controls.

(Zhang et al., 2017)

Class III allele. Eye disc size is intermediate between very small and wild type. Larval survival rate of homozygotes is 68% of heterozygotes with wild type.

(Wiersdorff et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Mad^8-2/Mad[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Nlg4^UAS.cZa, Scer\GAL4^elav.PU

(Zhang et al., 2017)

Mad^8-2/Mad[+] is a suppressor of abnormal neuroanatomy phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Mad^8-2/Mad[+] is a suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Other

Statement

Reference

Mad^8-2/Mad[+], Nlg4^KO10 has abnormal neuroanatomy | third instar larval stage phenotype

(Zhang et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Mad^8-2/Mad[+] is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Nlg4^UAS.cZa, Scer\GAL4^RapGAP1-OK6

(Zhang et al., 2017)

Mad^8-2/Mad[+] is a suppressor of eye disc phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Mad^8-2/Mad[+] is a suppressor of glial cell phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Mad^8-2/Mad[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Other

Statement

Reference

Mad^8-2/Mad[+], Nlg4^KO10 has NMJ bouton | third instar larval stage phenotype

(Zhang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased bouton and satellite bouton number characteristic for third instar larvae expressing two copies of Nlg4^Scer\UAS.cZa under the control of Scer\GAL4^RapGAP1-OK6 is suppressed by combination with a single copy of Mad^8-2.

Although none of the larvae heterozygous for either Nlg4^KO10 or Mad^8-2 display any significant difference in the number or size of boutons on neuromuscular junctions compared to controls, Mad^8-2/+;Nlg4^KO10/+ double heterozygotes show reduced bouton number and increased bouton size.

(Zhang et al., 2017)

The glial overmigration phenotype seen in spin¹⁰⁴⁰³ eye discs is significantly suppressed if the animals are also heterozygous for Mad^8-2.

(Yuva-Aydemir et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7324

w^*; Mad^8-2 P{neoFRT}40A/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mad^8-2

(Chen et al., 2022, Li et al., 2020, La Marca et al., 2019, Huang et al., 2011, Christensen and Cook, 2007.3.22, Christensen and Cook, 2006.8.29, Christensen and Cook, 2006.8.29, Christensen and Cook, 2006.8.29, Christensen and Cook, 2006.12.5, Oishi et al., 2006)

mad8-2

(Zhang et al., 2017)

mad^8-2

(Davies et al., 2013, Yuva-Aydemir et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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