FlyBase Allele Report: Dmel\Nlg2[UAS.cSa]

General Information

Symbol

Dmel\Nlg2^UAS.cSa

Species

D. melanogaster

Name

FlyBase ID

FBal0258112

Feature type

allele

Associated gene

Dmel\Nlg2

Associated Insertion(s)

Carried in Construct

P{UAS-Nlg2.S}

Key Links

Transgenic product class

cDNA

(Sun et al., 2011)

wild_type

(Sun et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sun et al., 2011)

wild_type

(Sun et al., 2011)

Mutagen

in vitro construct

(Sun et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Nlg2.S}

Cytology

Description

UASt regulatory sequences drive expression of full-length Nlg2 cDNA sequences.

(Sun et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Nlg2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^C57

(Chen et al., 2012)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^how-24B

(Chen et al., 2012)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^αTub84B.PL

(Chen et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Nlg2^Scer\UAS.cSa postsynaptically, under the control of either Scer\GAL4^C57 or Scer\GAL4^how-24B, results in a reduction in bouton number at the neuromuscular junction in third instar larvae.

Expression of Nlg2^Scer\UAS.cSa presynaptically, under the control of Scer\GAL4^elav.PU, has no effect on bouton number at the third larval instar neuromuscular junction.

Expression of Nlg2^Scer\UAS.cSa in both neurons and muscles, under the control of Scer\GAL4^αTub84B.PL, results in an increase in bouton number at the neuromuscular junction in third instar larvae.

(Chen et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^αTub84B.PL/Nlg2^UAS.cSa rescues Nlg2^CL2

(Chen et al., 2012)

Scer\GAL4^αTub84B.PL/Nlg2^UAS.cSa rescues Nlg2^KO70

(Chen et al., 2012)

Scer\GAL4^how-24B/Nlg2^UAS.cSa rescues Nlg2^GD962

(Sun et al., 2011)

Nlg2^UAS.cSa/Scer\GAL4^C57 rescues Nlg2^GD962

(Sun et al., 2011)

Fails to rescue

Nlg2^UAS.cSa/Scer\GAL4^C57 fails to rescue Nlg2^CL2

(Chen et al., 2012)

Scer\GAL4^how-24B/Nlg2^UAS.cSa fails to rescue Nlg2^CL2

(Chen et al., 2012)

Scer\GAL4^elav.PU/Nlg2^UAS.cSa fails to rescue Nlg2^CL2

(Chen et al., 2012)

Nlg2^UAS.cSa/Scer\GAL4^C57 fails to rescue Nlg2^KO70

(Chen et al., 2012)

Scer\GAL4^how-24B/Nlg2^UAS.cSa fails to rescue Nlg2^KO70

(Chen et al., 2012)

Comments

Expression of Nlg2^Scer\UAS.cSa postsynaptically, under the control of either Scer\GAL4^C57 or Scer\GAL4^how-24B, does not rescue the reduction in bouton number seen at the neuromuscular junction in Nlg2^CL2 third instar larvae. Expression of Nlg2^Scer\UAS.cSa presynaptically, under the control of Scer\GAL4^elav.PU, also fails to rescue this defect. However, expression of Nlg2^Scer\UAS.cSa in both neurons and muscles, under the control of Scer\GAL4^αTub84B.PL, restores bouton number at the neuromuscular junction in Nlg2^CL2 third instar larvae to wild-type levels.

Expression of Nlg2^Scer\UAS.cSa postsynaptically, under the control of either Scer\GAL4^C57 or Scer\GAL4^how-24B, does not rescue the reduction in bouton number seen at the neuromuscular junction in Nlg2^KO70 third instar larvae. However, expression of Nlg2^Scer\UAS.cSa in both neurons and muscles, under the control of Scer\GAL4^αTub84B.PL, restores bouton number at the neuromuscular junction in Nlg2^KO70 third instar larvae to wild-type levels.

Expression of Nlg2^Scer\UAS.cSa under the control of either Scer\GAL4^how-24B or Scer\GAL4^elav.PU does not rescue the reduced EJP amplitude seen at the neuromuscular junction in Nlg2^CL2 and Nlg2^KO70 third instar larvae. Expression under the control of Scer\GAL4^αTub84B.PL rescues the EJP amplitude to wild type.

(Chen et al., 2012)

Co-expression of neuroligin^Scer\UAS.cSa by either Scer\GAL4^how-24B or Scer\GAL4^C57 rescues the bouton number and synaptic arborization deficits found upon expression of neuroligin^GD962.

(Sun et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nlg2^Scer\UAS.cSa

Nlg2^UAS.cSa

neuroligin^Scer\UAS.cSa

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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