FlyBase Allele Report: Dmel\Nlg2[CL2]

General Information

Symbol

Dmel\Nlg2^CL2

Species

D. melanogaster

Name

FlyBase ID

FBal0285172

Feature type

allele

Associated gene

Dmel\Nlg2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chen et al., 2012)

Mutagen

FLPase

(Chen et al., 2012)

Progenitor genotype

P{XP}d02251

(Chen et al., 2012)

PBac{WH}Polr1F^f04579

(Chen et al., 2012)

Caused by aberration

Df(2L)Nlg2^CL2

Cytology

Description

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them. This 32.6kb deletion removes Nlg2 and CG13773.

(Chen et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:6,874,419..6,907,036 [+]

Comment:

32.6 kb deletion of Nlg2 and Polr1F resulting from FLP-catalyzed recombination using the FRT-containing transposons P{XP}d02251 and PBac{WH}Polr1F^f04579.

(Chen et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Chen et al., 2012)

abnormal neurophysiology | third instar larval stage

(Chen et al., 2012)

viable

(Chen et al., 2012)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage

(Chen et al., 2012)

NMJ bouton | third instar larval stage

(Chen et al., 2012)

postsynaptic density

(Chen et al., 2012)

presynaptic active zone | increased number

(Chen et al., 2012)

subsynaptic reticulum

(Chen et al., 2012)

Detailed Description

Statement

Reference

The number of boutons at the neuromuscular junction is significantly reduced compared to wild type in mutant third instar larvae. The boutons are less defined than in wild type and are not well separated.

Several ultrastructural defects are seen in the mutant boutons compared to controls. The number of active zones per bouton is increased. The postsynaptic area (the space between the postsynaptic density and the subsynaptic reticulum (SSR)) is increased. The normalised width of the SSR is severely reduced. The total postsynaptic density length per unit perimeter is increased.

Homozygous third instar larval show a reduction in EJP amplitude at the neuromuscular junction compared to controls. mEJP amplitude is not significantly altered. Quantal content is significantly reduced.

(Chen et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Nlg2^CL2/+ ; Nrx-1²⁷³/+ double heterozygous third instar larvae have normal neuromuscular junction morphology.

Nlg2^CL2/Nlg2^CL2 ; Nrx-1²⁷³/Df(3R)5C1 double mutant third instar larvae have a significantly reduced number of boutons at the neuromuscular junction, with the reduction being indistinguishable from that seen for each single mutant combination. EJP amplitude is reduced in the double mutants to a similar degree as that seen in each single mutant.

(Chen et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nlg2^CL2 is rescued by Nlg2^{+tCH322-173I20}

(Chen et al., 2012)

Nlg2^CL2 is rescued by Scer\GAL4^αTub84B.PL/Nlg2^UAS.cSa

(Chen et al., 2012)

Not rescued by

Nlg2^CL2 is not rescued by Nlg2^UAS.cSa/Scer\GAL4^C57

(Chen et al., 2012)

Nlg2^CL2 is not rescued by Scer\GAL4^how-24B/Nlg2^UAS.cSa

(Chen et al., 2012)

Nlg2^CL2 is not rescued by Scer\GAL4^elav.PU/Nlg2^UAS.cSa

(Chen et al., 2012)

Comments

Nlg2^{+tCH322-173I20} rescues the reduction in number of boutons at the neuromuscular junction which is seen in Nlg2^CL2 third instar larvae. The ultrastructural defects of the boutons are also rescued.

Expression of Nlg2^Scer\UAS.cSa postsynaptically, under the control of either Scer\GAL4^C57 or Scer\GAL4^how-24B, does not rescue the reduction in bouton number seen at the neuromuscular junction in Nlg2^CL2 third instar larvae. Expression of Nlg2^Scer\UAS.cSa presynaptically, under the control of Scer\GAL4^elav.PU, also fails to rescue this defect. However, expression of Nlg2^Scer\UAS.cSa in both neurons and muscles, under the control of Scer\GAL4^αTub84B.PL, restores bouton number at the neuromuscular junction in Nlg2^CL2 third instar larvae to wild-type levels.

Expression of Nlg2^Scer\UAS.cSa under the control of either Scer\GAL4^how-24B or Scer\GAL4^elav.PU does not rescue the reduced EJP amplitude seen at the neuromuscular junction in Nlg2^CL2 third instar larvae. Expression under the control of Scer\GAL4^αTub84B.PL rescues the EJP amplitude to wild type.

(Chen et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nlg2^CL2

dnlg2^CL2

(Chen et al., 2012)

dnlg2^Δ

(Chen et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (1)

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