FlyBase Allele Report: Dmel\fh[UAS.cLa]

General Information

Symbol

Dmel\fh^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Llorens

FlyBase ID

FBal0260623

Feature type

allele

Associated gene

Dmel\fh

Associated Insertion(s)

Carried in Construct

P{UAS-fh.L}

Also Known As

UAS-fh

Key Links

Transgenic product class

wild_type

(Llorens et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Llorens et al., 2007)

Mutagen

in vitro construct

(Llorens et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-fh.L}

Cytology

Description

UASt regulatory sequences drive expression of the fh coding sequence.

(Llorens et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^Appl.G1a

(Navarro et al., 2011)

abnormal locomotor behavior, with Scer\GAL4^{neur-GAL4-A101}

(Navarro et al., 2011)

abnormal locomotor behavior, with Scer\GAL4^repo

(Navarro et al., 2011)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Edenharter et al., 2017)

abnormal locomotor behavior | adult stage, with Scer\GAL4^{neur-GAL4-A101}

(Llorens et al., 2007)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Toll-6-D42

(Llorens et al., 2007)

decreased cell number | adult stage | progressive, with Scer\GAL4^ple.PF

(Edenharter et al., 2017)

increased mortality, with Scer\GAL4^Act5C.PI

(Edenharter et al., 2017)

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

(Llorens et al., 2007)

lethal - all die before end of pupal stage, with Scer\GAL4^Act.PU

(Llorens et al., 2007)

lethal - all die before end of pupal stage, with Scer\GAL4^how-24B

(Llorens et al., 2007)

lethal - all die before end of pupal stage, with Scer\GAL4^Ugt36A1.PK

(Llorens et al., 2007)

short lived, with Scer\GAL4^Appl.G1a

(Navarro et al., 2011)

short lived, with Scer\GAL4^elav-C155

(Edenharter et al., 2017)

short lived, with Scer\GAL4^{neur-GAL4-A101}

(Navarro et al., 2011, Llorens et al., 2007)

short lived, with Scer\GAL4^repo

(Navarro et al., 2011)

short lived, with Scer\GAL4^Toll-6-D42

(Llorens et al., 2007)

some die during pupal stage, with Scer\GAL4^Act.PU

(Llorens et al., 2007)

some die during pupal stage, with Scer\GAL4^how-24B

(Llorens et al., 2007)

some die during pupal stage, with Scer\GAL4^Ugt36A1.PK

(Llorens et al., 2007)

some die during third instar larval stage, with Scer\GAL4^da.G32

(Llorens et al., 2007)

viable, with Scer\GAL4^c698a

(Llorens et al., 2007)

viable, with Scer\GAL4^Ddc.PL

(Llorens et al., 2007)

viable, with Scer\GAL4^{neur-GAL4-A101}

(Llorens et al., 2007)

viable, with Scer\GAL4^Toll-6-D42

(Llorens et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of fh^UAS.cLa under the control of Scer\GAL4^elav-C155, but not Scer\GAL4^ple.PF, leads to a significant decrease in the climbing ability of adults compared to controls. The expression under the control of Scer\GAL4^elav-C155 also leads to a significant increase in mitochondrial activity in adults but does not induce brain vacuolization in 35 days old adults, as compared to controls.

Expression under the control of Scer\GAL4^ple.PF induces a strong clustering of mitochondria in adult dopaminergic neurons (assessed by mito-GFP fluorescence intensity) and leads to a significant decrease in the number of PPL1 neurons in 60 days old adults, but not in 30 and 6 days old adults, as compared to controls; PPM1-2 or PPM3 neurons are not significantly affected, as compared to controls.

Expression under the control of Scer\GAL4^Act5C.PI leads to a decrease in the proportion of surviving third instar larvae, pupae and adults in standard food compared to controls.

(Edenharter et al., 2017)

Expression of fh^Scer\UAS.cLa in neural tissues via any one of Scer\GAL4^Appl.G1a, Scer\GAL4^{neur-GAL4-A101} or Scer\GAL4^repo results in statistically significant decline in the lifespan of flies.

Flies expressing fh^Scer\UAS.cLa with any one of Scer\GAL4^Appl.G1a, Scer\GAL4^{neur-GAL4-A101} or Scer\GAL4^repo exhibit reduced climbing ability in an age dependent manner compared with wild-type.

(Navarro et al., 2011)

Expression of fh^Scer\UAS.cLa under the control of either Scer\GAL4^{neur-GAL4-A101} or Scer\GAL4^D42 results in a reduction in the mean life span of 64% and 49% respectively, and a 85% and 60% decline in climbing ability respectively.

Stage 16 embryos expressing fh^Scer\UAS.cLa under the control of Scer\GAL4^da.G32 show defects in the somatic muscles; in almost all embryos, muscles dorsal acute 3, dorsal oblique 3 and dorsal oblique 4 are partially formed. 70% of the mutant embryos show defects in the pathfinding of motor axons (assayed by Fas2 staining). 10% of the mutant embryos show an increase in the number of sensory ventral neurons and axonal pathfinding defects in the sensory nerves.

Stage 17 embryos expressing fh^Scer\UAS.cLa under the control of Scer\GAL4^Dot.PK show loss of pericardial cells.

(Llorens et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, fh^UAS.cLa has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Cat^{Tag:Mito(Oat)}, Scer\GAL4^elav-C155

(Edenharter et al., 2017)

Scer\GAL4^elav-C155, fh^UAS.cLa has abnormal locomotor behavior | adult stage phenotype, suppressible by mfrn^GD336/Scer\GAL4^elav-C155

(Edenharter et al., 2017)

Scer\GAL4^{neur-GAL4-A101}, fh^UAS.cLa has short lived phenotype, suppressible by Cat^{Tag:Mito(Oat)}

(Navarro et al., 2011)

Scer\GAL4^repo, fh^UAS.cLa has abnormal locomotor behavior phenotype, suppressible by Cat^{Tag:Mito(Oat)}

(Navarro et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, fh^UAS.cLa has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Drp1^UAS.cDa, Scer\GAL4^elav-C155

(Edenharter et al., 2017)

Scer\GAL4^repo, fh^UAS.cLa has short lived phenotype, non-suppressible by Cat^{Tag:Mito(Oat)}

(Navarro et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Constitutive expression of Cat^T:Mito-oat significantly extends the lifespan of flies expressing fh^Scer\UAS.cLa under the control of Scer\GAL4^{neur-GAL4-A101}.

Constitutive expression of Cat^T:Mito-oat fails to extend the lifespan of flies expressing fh^Scer\UAS.cLa under the control of Scer\GAL4^repo.

Constitutive expression of Cat^T:Mito-oat ameliorates the climbing deficiency that is induced by expression of fh^Scer\UAS.cLa in glial cells under the control of Scer\GAL4^repo.

(Navarro et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

fh^UAS.cLa/Scer\GAL4^repo.PU rescues fh^RNAi.UAS.cAa

(Navarro et al., 2015)

Comments

Expression of fh^Scer\UAS.cLa rescues the motor defects observed in flies expressing fh^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^repo.PU.

(Navarro et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

fh^Scer\UAS.cLa

fh^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Llorens

Secondary FlyBase IDs

References (6)

Report Sections

Open Close