A 6kb genomic fragment of dco carrying a L39Q amino acid change.
Amino acid replacement: L39Q.
wing pouch, with dco3
The presence of dcot.L39Q in a dcole88/Df(3R)A177der20 background results in an extended larval development (10 days compared to 5 days for wild-type larvae) associated with a striking overgrowth phenotype in larval imaginal discs. Whereas the wild-type disc stop growing when they reach the appropriate size, the mutant discs continue growing during the extended larval period and before pupariation they reach about three times the normal final size. Although the mutant larvae eventually pupate they do not proceed through metamorphosis, and the pupae die without any signs of adult development. The overall morphology of these mutants is abnormal, with extensive rippling and loss of normal morphological features including the wing pouch. These mutant discs retain their monolayered epithelial structure showing that they exhibit a hyperplastic overgrowth phenotype.
The presence of dcot.L39Q and dcot.S101R in a dcole88/Df(3R)A177der20 background results in an extended larval development period. The discs do not grow larger than normal, but the tissue seems to disintegrate in places, indicating a loss of tissue integrity.
The presence of dcot.L39Q and dcot.S101R in a dcole88/Df(3R)A177der20 background results in a very mild dominant effect on a crossvein in the adult wing, with less than 15% penetrance.
Df(3R)A177der20/dcole88, dcoL39Q has crossvein phenotype, enhanceable by wtsP2
Df(3R)A177der20/dcole88, dcoL39Q has wing phenotype, enhanceable by wtsP2
A wtsP2 heterozygous background significantly enhances the mild dominant wing crossvein defect seen in dcole88/Df(3R)A177der20 flies carrying dcot.L39Q. The extra vein fragment is present in more than 90% of the transheterozygotes. The fragment is usually enlarged, and most of the wings show additional extra vein material not present in either heterozygous combination. Wing size is also significantly increased in these flies.
