FlyBase Allele Report: Dmel\Cdk1[GL00262]

General Information

Symbol

Dmel\Cdk1^GL00262

Species

D. melanogaster

Name

FlyBase ID

FBal0262732

Feature type

allele

Associated gene

Dmel\Cdk1

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00262}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00262}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00262

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Sopko et al., 2014)

hypoplasia, with Scer\GAL4^mirr-DE

(Wang et al., 2015)

Phenotype Manifest In

eye disc | larval stage, with Scer\GAL4^mirr-DE

(Wang et al., 2015)

Detailed Description

Statement

Reference

Expression of Cdk1^GL00262 under the control of Scer\GAL4^mirr-DE attenuates larval eye disc growth.

(Wang et al., 2015)

Females heterozygous for Cdk1^GL00262 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to Cdk1^GL00262 males results in: no eggs; egg/embryonic phenotype: no vitellogenic egg chambers; oogenesis defect.

(Sopko et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Cdk1^GL00262, Scer\GAL4^e22c is a non-suppressor of abnormal cell shape | third instar larval stage phenotype of Scer\GAL4^e22c, stck^NIG.7954R

(Wang et al., 2015)

Cdk1^GL00262, Scer\GAL4^e22c is a non-suppressor of increased cell size | third instar larval stage phenotype of Scer\GAL4^e22c, stck^NIG.7954R

(Wang et al., 2015)

Cdk1^GL00262, Scer\GAL4^pnr-MD237 is a non-suppressor of abnormal cell shape | third instar larval stage phenotype of Scer\GAL4^pnr-MD237, hep^CA.UAS

(Wang et al., 2015)

Cdk1^GL00262, Scer\GAL4^pnr-MD237 is a non-suppressor of increased cell size | third instar larval stage phenotype of Scer\GAL4^pnr-MD237, hep^CA.UAS

(Wang et al., 2015)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Cdk1^GL00262, Scer\GAL4^e22c is a non-suppressor of embryonic/larval epidermis | third instar larval stage phenotype of Scer\GAL4^e22c, stck^NIG.7954R

(Wang et al., 2015)

Cdk1^GL00262, Scer\GAL4^pnr-MD237 is a non-suppressor of embryonic/larval epidermis | third instar larval stage phenotype of Scer\GAL4^pnr-MD237, hep^CA.UAS

(Wang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of abnormal large syncytia in larval epidermis characteristic for third instar larvae expressing stck^NIG.7954R under the control of Scer\GAL4^e22c is not suppressed by co-expression of any of Cdk1^GL00262.

The formation of giant syncytia in the patches of dorsal larval epidermis expressing hep^CA.Scer\UAS under the control of Scer\GAL4^pnr-MD237 (using tub-Gal80ts to limit the expression to 16 hr-long pulse during the third instar) is not suppressed by co-expression of Cdk1^GL00262, the number of nuclei within the syncytia is also not changed.

(Wang et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

35350