FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\zip4
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General Information
Symbol
Dmel\zip4
Species
D. melanogaster
Name
FlyBase ID
FBal0263484
Feature type
allele
Associated gene
Dmel\zip
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Okumura et al., 2010)
Progenitor genotype
Cytology
Description

Amino acid replacement: R1447term.

(Okumura et al., 2010)

The mutation results in a truncated protein that lacks part of the coiled coil domain.

(Okumura et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:24,993,627..24,993,627 [-]
Nucleotide change:

C24993627T

Amino acid change:

R1447term | zip-PA; R1402term | zip-PB; R1362term | zip-PC; R1407term | zip-PD; R1362term | zip-PE; R1370term | zip-PF; R1362term | zip-PG; R1370term | zip-PH; R1415term | zip-PI

Reported amino acid change:

R1447term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Okumura et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal axis specification | embryonic stage 15 (with Df(2R)gsb)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 15 (with zip1)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 15 (with zip2)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 15 (with zip3)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 15 | recessive

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 (with Df(2R)gsb)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 (with zip1)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 (with zip2)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 (with zip3)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 | recessive

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 (with Df(2R)gsb)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 (with zip1)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 (with zip2)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 (with zip3)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 | recessive

      (Okumura et al., 2010)

      lethal - all die before end of larval stage

      (Okumura et al., 2010)

      lethal - all die before end of larval stage (with Df(2R)gsb)

      (Okumura et al., 2010)

      lethal - all die before end of larval stage (with zip1)

      (Okumura et al., 2010)

      lethal - all die before end of larval stage (with zip2)

      (Okumura et al., 2010)

      lethal - all die before end of larval stage (with zip3)

      (Okumura et al., 2010)

      some die during larval stage

      (Okumura et al., 2010)

      some die during larval stage (with Df(2R)gsb)

      (Okumura et al., 2010)

      some die during larval stage (with zip1)

      (Okumura et al., 2010)

      some die during larval stage (with zip2)

      (Okumura et al., 2010)

      some die during larval stage (with zip3)

      (Okumura et al., 2010)
      Phenotype Manifest In

      embryonic/larval anterior midgut | embryonic stage 15

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 15 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 15 (with zip1)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 15 (with zip2)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 15 (with zip3)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 16

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 16 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 16 (with zip1)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 16 (with zip2)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 16 (with zip3)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 17

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 17 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 17 (with zip1)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 17 (with zip2)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 17 (with zip3)

      (Okumura et al., 2010)

      embryonic esophagus | embryonic stage 17

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 15

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 15 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 15 (with zip1)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 15 (with zip2)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 15 (with zip3)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 16

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 16 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 16 (with zip1)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 16 (with zip2)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 16 (with zip3)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 17

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 17 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 17 (with zip1)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 17 (with zip2)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 17 (with zip3)

      (Okumura et al., 2010)
      Detailed Description
      Statement
      Reference

      zip4 homozygous mutants show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      zip4 heterozygotes appear wild-type.

      zip3/zip4 embryos show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      zip4/zip1 embryos show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      zip4/zip2 embryos show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      zip4/Df(2R)gsb embryos show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      Approximately 20% of zip4 stage 17 embryos show left-right inversion in the looping of the esophagus.

      zip4 mutants exhibit dorsal closure defects.

      (Okumura et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      zip4 is rescued by zipUAS.mGFP6/Scer\GAL4wg.PM

      (Okumura et al., 2010)
      Comments

      Expression of zipScer\UAS.T:Avic\GFP driven by Scer\GAL4wg.PM suppresses the dorsal closure defects found in zip4 homozygotes although the no-rotation phenotype is not suppressed.

      (Okumura et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      zip4
      (Okumura et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)