FlyBase Allele Report: Dmel\CanA-14F[KO]

General Information

Symbol

Dmel\CanA-14F^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0263887

Feature type

allele

Associated gene

Dmel\CanA-14F

Associated Insertion(s)

TI{TI}CanA-14F^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Nakai et al., 2011)

Mutagen

ends-out gene targeting

(Nakai et al., 2011)

I-SceI

(Nakai et al., 2011)

FLPase

(Nakai et al., 2011)

Progenitor genotype

Associated Insertion(s)

TI{TI}CanA-14F^KO

Cytology

Description

Precise replacement of the CanA-14F coding sequence by a loxP cassette containing a w^+* marker. An attP site is present downstream of the 3' loxP site to allow future integration of constructs into the knock-out site.

(Nakai et al., 2011)

Allele components

Component

Use(s)

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior

(Nakai et al., 2011)

abnormal sleep

(Nakai et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

CanA-14F^KO mutants exhibit a three-fold increase in total daily activity and a two-fold decrease in total sleep, both in LD and DD conditions.

(Nakai et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

CanA-14F^KO has abnormal sleep phenotype, enhanceable by sra^GS3080/sra^KO

(Nakai et al., 2011)

Suppressed by

Statement

Reference

CanA-14F^KO, Pp2B-14D^KO has abnormal meiotic cell cycle | germline clone phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pp2B-14D^UASp.cTa

(Takeo et al., 2012)

NOT suppressed by

Statement

Reference

CanA-14F^KO, Pp2B-14D^KO has abnormal meiotic cell cycle | germline clone phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/Pp2B-14D^DN.UASp

(Takeo et al., 2012)

Suppressor of

Statement

Reference

CanA-14F^KO is a suppressor | partially of abnormal sleep phenotype of sra^GS3080/sra^KO

(Nakai et al., 2011)

Other

Statement

Reference

CanA-14F^KO, Pp2B-14D^KO has lethal | larval stage phenotype

(Takeo et al., 2012)

CanA-14F^KO, Pp2B-14D^KO has sterile | germline clone phenotype

(Takeo et al., 2012)

CanA-14F^KO, Pp2B-14D^KO has abnormal meiotic cell cycle | germline clone phenotype

(Takeo et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Pp2B-14D^KO CanA-14F^KO double mutant flies are lethal at the larval stage.

Mature oocytes generated from Pp2B-14D^KO CanA-14F^KO double mutant germline clones have a normal metaphase I chromosome configuration. Mothers bearing Pp2B-14D^KO CanA-14F^KO mutant germline clones are sterile and the eggs exhibit a meiotic arrested phenotype at anaphase I.

Expression of Pp2B-14D^{Scer\UAS.P\T.cTa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the meiotic defects in 97.3% of Pp2B-14D^KO CanA-14F^KO double mutant eggs.

Expression of Pp2B-14D^{DN.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the meiotic defects in 97.3% of Pp2B-14D^KO CanA-14F^KO double mutant eggs.

(Takeo et al., 2012)

A CanA-14F^KO background partially suppresses the reduction in total sleep found in sra^GS3080/sra^KO mutants.

(Nakai et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CanA-14F-KO

(Agrawal and Hardin, 2016)

CanA-14F^KO

(Nakai et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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