FlyBase Allele Report: Dmel\zip[Rod.UAS.GFP]

General Information

Symbol

Dmel\zip^Rod.UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0264296

Feature type

allele

Associated gene

Dmel\zip

Associated Insertion(s)

Carried in Construct

P{UAS-zip.GFP.Rod}

Key Links

Transgenic product class

inframe_deletion

(Franke et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Franke et al., 2010)

Mutagen

in vitro construct

(Franke et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-zip.GFP.Rod}

Cytology

Description

UASt regulatory sequences drive expression of amino acids 909-1972 of zip which are tagged at the N-terminal end with GFP.

(Franke et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

zip

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ap-md544

(Franke et al., 2010)

visible, with Scer\GAL4^en-e16E

(Franke et al., 2010)

visible, with Scer\GAL4^en-e16E, zip¹

(Franke et al., 2010)

Phenotype Manifest In

macrochaeta, with Scer\GAL4^ap-md544

(Franke et al., 2010)

microchaeta, with Scer\GAL4^ap-md544

(Franke et al., 2010)

trichome, with Scer\GAL4^ap-md544

(Franke et al., 2010)

trichome | increased number, with Scer\GAL4^ap-md544

(Franke et al., 2010)

wing, with Scer\GAL4^en-e16E, zip¹

(Franke et al., 2010)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Franke et al., 2010)

wing hair | increased number, with Scer\GAL4^en-e16E

(Franke et al., 2010)

Detailed Description

Statement

Reference

Expression of a single copy of zip^{Rod.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^en-e16E results in the posterior wing margin having a slightly wavy appearance in the plane of the wing and the density of wing hairs on the posterior wing surface is not consistently uniform. Cells in the posterior wing have a multiple wing hair phenotype. Expression of two copies of zip^{Rod.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^en-e16E results in a more severe phenotype; 85% of wings show loss of posterior compartment tissue.

Expression of a single copy of zip^{Rod.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^en-e16E in a zip¹/+ background results in a more severe phenotype than expression in a wild-type background.

Expression of zip^{Rod.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ap-md544 results in epidermal cells of the thorax having multiple or branched setae. Microchaetae and macrochaetae on the thorax may also show a branched phenotype, or multiple chaetae extending from a single socket cell may also be seen.

(Franke et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, zip^Rod.UAS.GFP has visible phenotype, enhanceable by Scer\GAL4^en-e16E/fz^UAS.cAa

(Franke et al., 2010)

Scer\GAL4^en-e16E, zip^Rod.UAS.GFP has visible phenotype, enhanceable by Scer\GAL4^en-e16E/ck^UAS.GFP

(Franke et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, zip^Rod.UAS.GFP has visible phenotype, suppressible by ck⁷/ck[+]

(Franke et al., 2010)

Scer\GAL4^en-e16E, zip^Rod.UAS.GFP has visible phenotype, suppressible by ck[+]/ck¹³

(Franke et al., 2010)

Other

Statement

Reference

Scer\GAL4^en-e16E, fz^UAS.cAa, zip^Rod.UAS.GFP has abnormal planar polarity phenotype

(Franke et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, zip^Rod.UAS.GFP has wing hair | increased number phenotype, enhanceable by Scer\GAL4^en-e16E/fz^UAS.cAa

(Franke et al., 2010)

Scer\GAL4^en-e16E, zip^Rod.UAS.GFP has wing hair | increased number phenotype, enhanceable by Scer\GAL4^en-e16E/ck^UAS.GFP

(Franke et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, zip^Rod.UAS.GFP has wing hair | increased number phenotype, suppressible by ck⁷/ck[+]

(Franke et al., 2010)

Scer\GAL4^en-e16E, zip^Rod.UAS.GFP has wing hair | increased number phenotype, suppressible by ck[+]/ck¹³

(Franke et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of fz^Scer\UAS.cAa enhances the penetrance of the multiple wing hair phenotype caused by expression of zip^{Rod.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^en-e16E. In addition, the double mutant wings also show polarity defects.

The multiple wing hair phenotype caused by expression of zip^{Rod.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^en-e16E is suppressed by ck⁷/+ and by ck¹³/+.

Co-expression of ck^{Scer\UAS.T:Avic\GFP} enhances the penetrance of the multiple wing hair phenotype caused by expression of zip^{Rod.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^en-e16E.

(Franke et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^sqh.PW/zip^Rod.UAS.GFP fails to rescue zip²/zip¹

(Franke et al., 2010)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

zip^{Rod.Scer\UAS.T:Avic\GFP}

zip^Rod.UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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