FlyBase Allele Report: Dmel\trr[HMS01019]

General Information

Symbol

Dmel\trr^HMS01019

Species

D. melanogaster

Name

FlyBase ID

FBal0266448

Feature type

allele

Associated gene

Dmel\trr

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01019}

Also Known As

UAS-Trr-RNAi, UAS-RNAi-trr

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01019}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01019

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Kleefstra syndrome 2

CEC

(Koemans et al., 2017)

model of congenital heart disease

CEA

(Huang et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: "KLEEFSTRA SYNDROME 2" is associated with human ortholog KMT2.

(Koemans et al., 2017)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

male pronucleus | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}

(Prudêncio et al., 2018)

polar body nucleus, with Scer\GAL4^{VP16.nanos.UTR}

(Prudêncio et al., 2018)

wing, with Scer\GAL4^dpp.PU

(Qing et al., 2014)

Detailed Description

Statement

Reference

The expression of trr^HMS01019 under the control of Scer\GAL4^VP16.nos.UTR leads to female sterility as eggs do not enter embryogenesis because they do not initiate embryo mitotic divisions. The eggs do not support male pronucleus formation (and Protamine B is not removed). Meiosis is invariably completed but seems unsuccessful (shown by abnormal polar body rosettes). Ovary morphology, ovarian follicle development, mature egg size, prophase I oocyte chromatin architecture and meiotic chromosome retraction into the metaphase I plate are indistinguishable from controls.

Expression under the control of Scer\GAL4^bam.PU does not affect male fertility,

(Prudêncio et al., 2018)

Individuals expressing trr^HMS01019 under the control of Scer\GAL4^GMR14H06 do not show the expected reduction in courtship in rejected flies compared to naive flies normally observed in controls and, as a result, show a significantly lower learning index. These individuals do not show gross morphological defects in the mushroom body. Unlike in controls, there is .

(Koemans et al., 2017)

Expression of trr^HMS01019 under the control of Scer\GAL4^dpp.PU results in a significant decrease in the width of the dpp expression domain in adult wings (the region between veins L3 and L4).

(Qing et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, trr^HMS01019 has female sterile phenotype, non-suppressible by Idgf4^d00895, Scer\GAL4^{VP16.nanos.UTR}

(Prudêncio et al., 2018)

Scer\GAL4^{VP16.nanos.UTR}, trr^HMS01019 has abnormal meiotic cell cycle | female phenotype, non-suppressible by Idgf4^d00895, Scer\GAL4^{VP16.nanos.UTR}

(Prudêncio et al., 2018)

Enhancer of

Statement

Reference

trr^HMS01019/Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, sd^UAS.cSa

(Qing et al., 2014)

Suppressor of

Statement

Reference

trr^HMS01019/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Scer\GAL4^GMR.PU, yki^UAS.cHa

(Qing et al., 2014)

NOT Suppressor of

Statement

Reference

trr^HMS01019/Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PU, wts^KK101055

(Qing et al., 2014)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, trr^HMS01019 has male pronucleus | maternal effect phenotype, non-suppressible by Idgf4^d00895, Scer\GAL4^{VP16.nanos.UTR}

(Prudêncio et al., 2018)

Scer\GAL4^{VP16.nanos.UTR}, trr^HMS01019 has polar body nucleus phenotype, non-suppressible by Idgf4^d00895, Scer\GAL4^{VP16.nanos.UTR}

(Prudêncio et al., 2018)

Enhancer of

Statement

Reference

trr^HMS01019/Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, sd^UAS.cSa

(Qing et al., 2014)

Suppressor of

Statement

Reference

trr^HMS01019/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, yki^UAS.cHa

(Qing et al., 2014)

trr^HMS01019/Scer\GAL4^Tub.PU is a suppressor of wing disc | somatic clone phenotype of hpo^42-48

(Qing et al., 2014)

NOT Suppressor of

Statement

Reference

trr^HMS01019/Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, wts^KK101055

(Qing et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of trr^HMS01019 suppresses the increase in eye size seen when yki^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PU.

Expression of trr^HMS01019 enhances the reduction in eye size seen when sd^Scer\UAS.cSa is expressed under the control of Scer\GAL4^GMR.PU.

Expression of trr^HMS01019 does not visibly suppress the increase in eye size seen when wts^KK101055 is expressed under the control of Scer\GAL4^GMR.PU.

Expression of trr^HMS01019 under the control of Scer\GAL4^tub.PU suppresses the overgrowth seen in hpo^42-48 mutant wing disc clones.

(Qing et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36916

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01019}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

trr^HMS01019

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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