FlyBase Allele Report: Dmel\Vps4[3B1]

General Information

Symbol

Dmel\Vps4^3B1

Species

D. melanogaster

Name

FlyBase ID

FBal0267322

Feature type

allele

Associated gene

Dmel\Vps4

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Legent et al., 2012)

Progenitor genotype

Cytology

Description

Amino acid replacement: E209K.

(Legent et al., 2015)

Nucleotide substitution: G1610A.

(Legent et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:17,909,296..17,909,296 [+]

Nucleotide change:

G17909296A

Reported nucleotide change:

G1610A

Amino acid change:

E209K | Vps4-PA; E209K | Vps4-PB

Reported amino acid change:

E209K

(Legent et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | somatic clone | third instar larval stage

(Legent et al., 2015)

visible | somatic clone

(Legent et al., 2012)

Phenotype Manifest In

eye | somatic clone

(Legent et al., 2012)

eye disc | somatic clone | third instar larval stage

(Legent et al., 2015)

ommatidium | somatic clone

(Legent et al., 2012)

photoreceptor cell R1 | somatic clone | third instar larval stage

(Legent et al., 2015)

photoreceptor cell R2 | somatic clone | third instar larval stage

(Legent et al., 2015)

photoreceptor cell R3 | somatic clone | third instar larval stage

(Legent et al., 2015)

photoreceptor cell R4 | somatic clone | third instar larval stage

(Legent et al., 2015)

photoreceptor cell R5 | somatic clone | third instar larval stage

(Legent et al., 2015)

photoreceptor cell R6 | somatic clone | third instar larval stage

(Legent et al., 2015)

photoreceptor cell R7 | somatic clone | third instar larval stage

(Legent et al., 2015)

photoreceptor cell R8 | somatic clone | third instar larval stage

(Legent et al., 2015)

wing disc | somatic clone | third instar larval stage

(Legent et al., 2015)

Detailed Description

Statement

Reference

Vps4^3B1 mutant clones in third instar larval eye discs undergo massive apoptosis. There is a reduction in the number of R8 photoreceptors compared to controls and R1-7 photoreceptor differentiation is disrupted.

Vps4^3B1 mutant clones in the wing disc survive poorly.

tub-GAL4

Vps4^3B1 mutant follicle cell clones are large in size, indicating normal survival of the mutant cells.

(Legent et al., 2015)

Ommatidia fail to differentiate in homozygous clones in the eye.

(Legent et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Vps4^3B1 has increased cell death | somatic clone | third instar larval stage phenotype, suppressible by Dronc^I29

(Legent et al., 2015)

Vps4^3B1 has increased cell death | somatic clone | third instar larval stage phenotype, suppressible by Ras85D^V12.UAS/Scer\GAL4^Tub.PU

(Legent et al., 2015)

NOT suppressed by

Statement

Reference

Vps4^3B1 has increased cell death | somatic clone | third instar larval stage phenotype, non-suppressible by hep^r75

(Legent et al., 2015)

Vps4^3B1 has increased cell death | somatic clone | third instar larval stage phenotype, non-suppressible by puc^UAS.cMa/Scer\GAL4^Tub.PU

(Legent et al., 2015)

Vps4^3B1 has increased cell death | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/btl::Egfr^λ.UAS

(Legent et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Vps4^3B1 has eye disc | somatic clone | third instar larval stage phenotype, suppressible by Dronc^I29

(Legent et al., 2015)

Vps4^3B1 has photoreceptor cell R8 | somatic clone | third instar larval stage phenotype, suppressible by Dronc^I29

(Legent et al., 2015)

Vps4^3B1 has eye disc | somatic clone | third instar larval stage phenotype, suppressible by Ras85D^V12.UAS/Scer\GAL4^Tub.PU

(Legent et al., 2015)

Vps4^3B1 has photoreceptor | somatic clone | third instar larval stage phenotype, suppressible by Ras85D^V12.UAS/Scer\GAL4^Tub.PU

(Legent et al., 2015)

NOT suppressed by

Statement

Reference

Vps4^3B1 has eye disc | somatic clone | third instar larval stage phenotype, non-suppressible by hep^r75

(Legent et al., 2015)

Vps4^3B1 has eye disc | somatic clone | third instar larval stage phenotype, non-suppressible by puc^UAS.cMa/Scer\GAL4^Tub.PU

(Legent et al., 2015)

Vps4^3B1 has photoreceptor | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/shi^1.UAS

(Legent et al., 2015)

Vps4^3B1 has eye disc | somatic clone | third instar larval stage phenotype, non-suppressible by shi^FL54/Dronc^I29

(Legent et al., 2015)

Vps4^3B1 has photoreceptor | somatic clone | third instar larval stage phenotype, non-suppressible by shi^FL54/Dronc^I29

(Legent et al., 2015)

Vps4^3B1 has photoreceptor | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/spi^s.UAS

(Legent et al., 2015)

Vps4^3B1 has eye disc | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/btl::Egfr^λ.UAS

(Legent et al., 2015)

Vps4^3B1 has photoreceptor | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/btl::Egfr^λ.UAS

(Legent et al., 2015)

Vps4^3B1 has eye disc | somatic clone | third instar larval stage phenotype, non-suppressible by shi^7C7

(Legent et al., 2015)

Vps4^3B1 has photoreceptor | somatic clone | third instar larval stage phenotype, non-suppressible by shi^7C7

(Legent et al., 2015)

Vps4^3B1 has eye disc | somatic clone | third instar larval stage phenotype, non-suppressible by shi^7C7/Dronc^I29

(Legent et al., 2015)

Vps4^3B1 has photoreceptor | somatic clone | third instar larval stage phenotype, non-suppressible by shi^7C7/Dronc^I29

(Legent et al., 2015)

Vps4^3B1 has eye disc | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/shi^1.UAS

(Legent et al., 2015)

Suppressor of

Statement

Reference

Vps4^3B1 is a suppressor of photoreceptor | increased number | somatic clone | third instar larval stage phenotype of shi^7C7

(Legent et al., 2015)

Other

Statement

Reference

Scer\GAL4^Tub.PU, Vps4^3B1, spi^s.UAS has photoreceptor | increased number | somatic clone | cell non-autonomous | third instar larval stage phenotype

(Legent et al., 2015)

Scer\GAL4^Tub.PU, Vps4^3B1, btl::Egfr^λ.UAS has photoreceptor | somatic clone | cell non-autonomous | third instar larval stage phenotype

(Legent et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Dronc^I29 largely suppresses the increase in apoptosis seen in Vps4^3B1 mutant eye disc clones. The reduction in R8 photoreceptor number is also suppressed.

hep^r75 does not suppress the photoreceptor differentiation defects or increased cell death seen in Vps4^3B1 mutant eye disc clones.

Expression of puc^Scer\UAS.cMa under the control of Scer\GAL4^tub.PU does not suppress the photoreceptor differentiation defects or increased cell death seen in Vps4^3B1 mutant eye disc clones.

Expression of spi^s.Scer\UAS does not suppress the photoreceptor differentiation defects seen in Vps4^3B1 mutant clones, but induces ectopic photoreceptor differentiation in the wild type cells surrounding the clones.

Expression of btl::Egfr^{Scer\UAS.T:λ\cI-DD} does not suppress the photoreceptor differentiation defects seen in Vps4^3B1 mutant clones, but induces premature photoreceptor differentiation in the wild type cells surrounding the clones. The increase in cell death seen in Vps4^3B1 mutant clones is not suppressed by btl::Egfr^{Scer\UAS.T:λ\cI-DD}.

Expression of Ras85D^V12.Scer\UAS suppresses the photoreceptor differentiation defects seen in Vps4^3B1 mutant clones. The increase in cell death seen in Vps4^3B1 mutant clones is also suppressed by Ras85D^V12.Scer\UAS.

Vps4^3B1 suppresses the increase in photoreceptor number seen in shi^7C7 mutant eye disc clones, with the double mutants showing the photoreceptor loss seen in Vps4^3B1 mutants alone. The same is seen in a Dronc^I29 mutant background.

Expression of shi^1.Scer\UAS under the control of Scer\GAL4^tub.PU does not suppress the photoreceptor loss seen in Vps4^3B1 mutant eye disc clones.

shi^FL54 does not suppress the photoreceptor loss seen in Vps4^3B1 mutant eye disc clones (in which cell death has been prevented using Dronc^I29).

(Legent et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Vps4^3B1 is rescued by Scer\GAL4^Tub.PU/Vps4^UAS.Tag:HA

(Legent et al., 2015)

Comments

Expression of Vps4^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tub.PU fully rescues the photoreceptor differentiation defects seen in Vps4^3B1 mutant clones.

98% of hemizygous Vps4^3B1 mutant males are rescued to viability by Vps4^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tub.PU.

(Legent et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63798

y¹ w^* Vps4^3B1 P{neoFRT}19A/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

3B1

(Legent et al., 2012)

3B1^3B1

Vps4^3B1

(Legent et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close