FlyBase Allele Report: Dmel\Rpi[RNAi.Sym.UAS]

General Information

Symbol

Dmel\Rpi^RNAi.Sym.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0268312

Feature type

allele

Associated gene

Dmel\Rpi

Associated Insertion(s)

Carried in Construct

P{UAS-Rpi.RNAi.Sym}

Key Links

Transgenic product class

RNAi_reagent

(Wang et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Wang et al., 2012)

Mutagen

in vitro construct

(Wang et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Rpi.RNAi.Sym}

Cytology

Description

UAS regulatory sequences positioned at either end of a 338bp fragment corresponding to part of the Rpi coding sequence, resulting in the production of a dsRNA targeting Rpi.

(Wang et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rpi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal oxidative stress response, with Scer\GAL4^Appl.G1a

(Wang et al., 2012)

abnormal oxidative stress response, with Scer\GAL4^GMR.PF

(Wang et al., 2012)

long lived, with Scer\GAL4^Appl.G1a

(Wang et al., 2012)

long lived, with Scer\GAL4^GMR.PF

(Wang et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Rpi^{dsRNA.Sym.Scer\UAS} under the control of either Scer\GAL4^Appl.G1a or Scer\GAL4^GMR.PF results in increased resistance to oxidative stress, which expression under the control of Scer\GAL4^da.G32 has no effect on oxidative stress resistance.

Expression of Rpi^{dsRNA.Sym.Scer\UAS} under the control of either Scer\GAL4^Appl.G1a or Scer\GAL4^GMR.PF results in a significant increase in mean lifespan compared to controls.

Expression of Rpi^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^da.G32 results in a modest enhancement of longevity of only 8-14% compared to controls.

Expression of Rpi^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 does not result in an increase in lifespan.

(Wang et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^Q108.UAS

(Wang et al., 2012)

Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^Q108.UAS

(Wang et al., 2012)

Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012)

Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012)

Other

Statement

Reference

G6pd^GD1203, Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF, Zzzz\CAG^Q108.UAS has visible phenotype

(Wang et al., 2012)

G6pd^GD1203, Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF, Zzzz\CAG^Q108.UAS has abnormal eye color phenotype

(Wang et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^Q108.UAS

(Wang et al., 2012)

Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of retina phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^Q108.UAS

(Wang et al., 2012)

Rpi^RNAi.Sym.UAS, Scer\GAL4^ninaE.PT is a suppressor of eye photoreceptor cell | conditional phenotype of Scer\GAL4^ninaE.PT, Zzzz\CAG^Q108.UAS

(Wang et al., 2012)

Rpi^RNAi.Sym.UAS, Scer\GAL4^ninaE.PT is a suppressor of rhabdomere | conditional phenotype of Scer\GAL4^ninaE.PT, Zzzz\CAG^Q108.UAS

(Wang et al., 2012)

Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Wang et al., 2012)

Other

Statement

Reference

G6pd^GD1203, Rpi^RNAi.Sym.UAS, Scer\GAL4^GMR.PF, Zzzz\CAG^Q108.UAS has eye phenotype

(Wang et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Rpi^{dsRNA.Sym.Scer\UAS} suppresses the rough eye and loss of eye pigmentation phenotypes seen in adults expressing Zzzz\CAG^{Q108.Scer\UAS} under the control of Scer\GAL4^GMR.PF. At the ultrastructural level, the structural integrity of the retina is improved, although vacuoles are still present.

Co-expression of Rpi^{dsRNA.Sym.Scer\UAS} suppresses the rough eye and loss of eye pigmentation phenotypes seen in adults expressing Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF.

The addition of Zw^GD1203 abolishes the ability of Rpi^{dsRNA.Sym.Scer\UAS} to suppress the eye defects caused by expression of Zzzz\CAG^{Q108.Scer\UAS} under the control of Scer\GAL4^GMR.PF, so that the resulting flies have a rough eye which shows loss of pigmentation.

Co-expression of Rpi^{dsRNA.Sym.Scer\UAS} significantly improves rhabdomere structure and rescues photoreceptor neurons in 4 week old flies expressing Zzzz\CAG^{Q108.Scer\UAS} under the control of Scer\GAL4^ninaE.PT.

(Wang et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rpi^RNAi.Sym.UAS

Rpi^{dsRNA.Sym.Scer\UAS}

Rpi^{dsRNA.Sym.UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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